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Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia

Jurnal Medik Veteriner · Vol. 8 No. 1 (2025) · pp. 153–166

DOI
10.20473/jmv.vol8.iss1.2025.153-166
ISSN
2581-012X, 2615-7497
Published
2025-04-01
Publisher
Universitas Airlangga
Language
eng
License
creativecommons.org/licenses/by-nc-sa/4.0

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.

Keywords

Collaboration network of the authors

40 authors · 215 co-authorship links · showing the 40 most connected

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  • Universitas Syiah Kuala
  • Institut Teknologi Medan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Muchammad YunusAffiliation unknown10
Aditya YudhanaAffiliation unknown9
Mustofa Helmi EffendiAffiliation unknown9
Sheila Marty YanestriaAffiliation unknown7
Setiawan KoesdartoAffiliation unknown7
Gunanti MahasriAffiliation unknown6
Siti Rani AyutiUniversitas Syiah Kuala6
Riza Zainuddin AhmadAffiliation unknown6
Jola RahmahaniAffiliation unknown6
Heni PuspitasariAffiliation unknown5
Nunuk Dyah Retno LastutiAffiliation unknown5
Ratih Novita PrajaAffiliation unknown4
Agus SunarsoAffiliation unknown4
Prima Ayu WibawatiAffiliation unknown4
Fedik Abdul RantamAffiliation unknown4
Sunaryo Hadi WarsitoAffiliation unknown3
Ira Sari YudaniayantiAffiliation unknown3
Viol Dhea KharismaAffiliation unknown2
Eko Agus SuyonoAffiliation unknown2
Arief BudimanAffiliation unknown2
Ulfah Juniarti SiregarAffiliation unknown2
Thomas Valentinus WidiyatnoAffiliation unknown2
Suwarno SuwarnoInstitut Teknologi Medan2
Sri HidanahAffiliation unknown2
Epy Muhammad LuqmanAffiliation unknown2
Imam MustofaAffiliation unknown2
Bambang Sektiari LukiswantoAffiliation unknown2
Ahmad AswinAffiliation unknown2

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://e-journal.unair.ac.id/JMV/oai, first harvested 9 Nov 2026, last seen 9 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia</dc:title>
	<dc:creator>Kurniawan, Muhammad 'Ahdi</dc:creator>
	<dc:creator>Suwanti, Lucia Tri</dc:creator>
	<dc:creator>Mufasirin, Mufasirin</dc:creator>
	<dc:creator>Suprihati, Endang</dc:creator>
	<dc:creator>Hastutiek, Poedji</dc:creator>
	<dc:creator>Kusnoto, Kusnoto</dc:creator>
	<dc:creator>Ansori, Arif Nur Muhammad</dc:creator>
	<dc:creator>Puspitasari, Yulianna</dc:creator>
	<dc:creator>Khairullah, Aswin Rafif</dc:creator>
	<dc:creator>Moses, Ikechukwu Benjamin</dc:creator>
	<dc:creator>Pratama, Bima Putra</dc:creator>
	<dc:creator>Riwu, Katty Hendriana Priscilia</dc:creator>
	<dc:subject xml:lang="en-US">disease</dc:subject>
	<dc:subject xml:lang="en-US">Eimeria bovis</dc:subject>
	<dc:subject xml:lang="en-US">Eimeria zuernii</dc:subject>
	<dc:subject xml:lang="en-US">molecular</dc:subject>
	<dc:subject xml:lang="en-US">morphometric</dc:subject>
	<dc:description xml:lang="en-US">Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Airlangga</dc:publisher>
	<dc:date>2025-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://e-journal.unair.ac.id/JMV/article/view/60314</dc:identifier>
	<dc:identifier>10.20473/jmv.vol8.iss1.2025.153-166</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Medik Veteriner; Vol. 8 No. 1 (2025): April; 153-166</dc:source>
	<dc:source>2581-012X</dc:source>
	<dc:source>2615-7497</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://e-journal.unair.ac.id/JMV/article/view/60314/32232</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Muhammad 'Ahdi Kurniawan, Lucia Tri Suwanti, Mufasirin Mufasirin, Endang Suprihati, Poedji Hastutiek, Kusnoto Kusnoto, Arif Nur Muhammad Ansori, Yulianna Puspitasari, Aswin Rafif Khairullah, Ikechukwu Benjamin Moses, Bima Putra Pratama, Katty Hendriana Priscilia Riwu</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</dc:rights>
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