Journal SINTA 1
Pengaruh Suhu Ekstraksi terhadap Aktivitas Antioksidan Ekstrak Metanolik Eucheuma spinosum: Effect of Extraction Temperature on Antioxidant Activity of Methanolic Extract of Eucheuma spinosum
Jurnal Pengolahan Hasil Perikanan Indonesia · Vol. 24 No. 1 (2021) · pp. 1–10
- ISSN
- 2354-886X, 2303-2111
- Published
- 2021-04-05
- Publisher
- Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
- Language
- ind
Abstract
Indonesian
Eucheuma spinosum merupakan alga merah yang banyak dibudidayakan dan termasuk salah satu komoditas unggulan karena memiliki senyawa fenolik dan flavonoid yang mampu berperan sebagai antioksidan. Senyawa fenolik dan flavonoid pada E. spinosum dapat diperoleh melalui proses maserasi menggunakan metanol yang pada umumnya dilakukan pada suhu ruang. Proses ekstraksi untuk mendapatkan hasil maksimum dapat dipercepat dengan cara meningkatkan suhu ekstraksi. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan E. spinosum yang diekstraksi menggunakan metanol dengan suhu 55, 65, dan 75 °C. Sebelum diekstraksi rumput laut dicuci bersih dan dikeringkan pada oven bersuhu 40 °C selama 24 jam. Ekstraksi dilakukan dengan metanol 80 % (1:10, b/v). Parameter yang diuji pada penelitian ini yaitu rendemen, uji fitokimia, total fenolik, total flavonoid, dan aktivitas antioksidan meliputi uji DPPH dan FRAP. Dari data penelitian mengindikasikan bahwa suhu ekstraksi memengaruhi total fenolik maupun aktivitas antioksidan baik DPPH maupun FRAP, namun tidak berpengaruh terhadap rendemen dan total flavonoid. Suhu ekstraksi 55 °C memiliki daya antioksidan paling tinggi (IC50 DPPH sebesar 362,52±7,10 ppm, dan nilai FRAP sebesar 70,30±10,01 μM ekuivalen Fe(II)/g).
English
Eucheuma spinosum is red algae that is widely cultivated and is also one of the superior commodities because it has phenolic and flavonoid compounds that can act as antioxidants. Phenolic content and flavonoids in E. spinosum can be obtained through a maceration process with methanol as a solvent which is generally carried out at room temperature. The extraction process to obtain maximum yield can be accelerated by increasing the extraction temperature. This research aims to determine the antioxidant activity of E. spinosum extracted using methanol at temperatures of 55, 65, and 75 °C. Before extraction, the seaweed was washed and dried in an oven at 40 °C for 24 hours. Extraction was carried out with 80 % methanol in a ratio of 1:10 (w/v). The parameters analyzed in this research were yield, phytochemical test, total phenolic content (TPC), total flavonoids, antioxidant activity including DPPH and FRAP. The data indicated that the extraction temperature affected total phenolic and antioxidant activity of both DPPH and FRAP, but did not affect the yield and total flavonoids. Extraction temperature of 55 °C had higher antioxidant activity with an IC50 DPPH value 362.52±7.10 ppm and FRAP value 70.30±10.01 μM equivalent to Fe (II)/g.
Keywords
Collaboration network of the authors
40 authors · 83 co-authorship links · 10 across different organisations · showing the 40 most connected
Hover or tap a name to highlight its co-authors. Double-click to open the author's page.
- Universitas Islam Negeri Sulthan Thaha Saifuddin Jambi
- Universitas Jambi
- National Research and Innovation Agency
- Politeknik Negeri Sriwijaya
- Affiliation unknown
- Different organisations
- Same organisation
- Affiliation unknown
- Thicker line = more joint articles · bigger circle = more articles
Show as table
| Co-author | Organisation | Joint articles |
|---|---|---|
| Siti Ari Budhiyanti | Affiliation unknown | 3 |
| Masagus Muhammad Prima Putra | Affiliation unknown | 2 |
| U. Ustadi | Affiliation unknown | 2 |
| Nurfitri Ekantari | Affiliation unknown | 2 |
| Titin Agustin Nengsih | Universitas Islam Negeri Sulthan Thaha Saifuddin Jambi | 1 |
| Muharoma Fahnur Ihsandi | Universitas Islam Negeri Sulthan Thaha Saifuddin Jambi | 1 |
| Imam Arifa’illah Syaiful Huda | Universitas Islam Negeri Sulthan Thaha Saifuddin Jambi | 1 |
| Wahyu Rizky Andhifani | National Research and Innovation Agency | 1 |
| Benny Agusti Putra | Universitas Islam Negeri Sulthan Thaha Saifuddin Jambi | 1 |
| Anandita Perwita Kurniawan | Affiliation unknown | 1 |
| Indun Dewi Puspita | Affiliation unknown | 1 |
| Venny Agustin | Politeknik Negeri Sriwijaya | 1 |
| ALIM ISNANSETYO | Affiliation unknown | 1 |
| INDAH ISTIQOMAH | Affiliation unknown | 1 |
| Toni Kuswoyo | Affiliation unknown | 1 |
| Muwantoko Murwantoko | Affiliation unknown | 1 |
| Nungky Mufarocha | Affiliation unknown | 1 |
| Antarif Kusuma Brata | Affiliation unknown | 1 |
| Maria Madalena | Affiliation unknown | 1 |
| Yusuf Kalingga Murda | Affiliation unknown | 1 |
| Ervika Rahayu Novita Herwati | Affiliation unknown | 1 |
| Almira Islamei Pratiwi | Affiliation unknown | 1 |
| Bambang Retno Aji | Affiliation unknown | 1 |
| Rani Laksanawati | Affiliation unknown | 1 |
| Annisa Ajeng Maharani | Affiliation unknown | 1 |
| Ivana M. Diharningrum | Affiliation unknown | 1 |
| Rizqi Wahyu Herdianto | Affiliation unknown | 1 |
| Amir Mugozin | Affiliation unknown | 1 |
| Asnani | Affiliation unknown | 1 |
| Kasmiati | Affiliation unknown | 1 |
| nurjanah nurjanah | Affiliation unknown | 1 |
| Zuriat Zuriat | Affiliation unknown | 1 |
| Rina Syafitri | Affiliation unknown | 1 |
| Mohamad Gazali | Affiliation unknown | 1 |
| Mila Novalita Suhardani | Affiliation unknown | 1 |
| May Khoirunnisa Setiadi | Affiliation unknown | 1 |
| Husna Vannisa Sunandar | Affiliation unknown | 1 |
| Elfrida Juliana Nababan | Affiliation unknown | 1 |
Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.