Aktivitas antioksidan dan total fenol rumput laut Sargassum sp. dari Perairan Simeulue Aceh: Total Phenolic Content and Antioxidant Activity of Seaweed Sargassum in Simeulue Water, Aceh

Abstract

Indonesian

Sargassum sp merupakan jenis rumput laut yang tumbuh subur secara alami di perairan Indonesia, termasuk di Perairan Simeulue, Provinsi Aceh. Sargassum sp. mengandung berbagai senyawa bioaktif yaitu fenol yang berpotensi sebagai antioksidan. Sargassum sp. di Perairan Simeulue belum dimanfaatkan oleh masyarakat setempat dan belum ada laporan ilmiah tentang total fenol dan aktivitas antioksidan dari rumput laut tersebut. Tujuan penelitian ini menentukan aktivitas antioksidan dan total fenol Sargassum sp yang terdapat di Perairan Simeulue. Metode penelitian meliputi pengambilan sampel rumput laut, preparasi dan ekstraksi sampel rumput laut, uji fitokimia, analisis total fenol dan analisis aktivitas antioksidan rumput laut. Analisis data menggunakan analisis regresi linear untuk melihat hubungan total fenol dengan aktivitas antioksidan DPPH Sargassum. Hasil penelitian menunjukkan bahwa total fenol dan aktivitas antioksidan lima jenis rumput laut genus Sargassum (S. muticum, S. binderi, S. crassifolium, S. granuliferum, S. fluitans) di Perairan Simeulue menunjukkan nilai yang bervariasi. Total fenol 7,45-9,02 mg GAE/g dengan nilai tertinggi terdapat pada S. binderi dan nilai terendah pada S. fluitans. Aktivitas antioksidan 74,7-152,4 μg/mL dengan nilai tertinggi terdapat pada S. binderi, sedangkan nilai terendah terdapat pada S. fluitans. Hasil analisis korelasi (R2 = 0,9059) menunjukkan bahwa aktivitas antioksidan rumput laut genus Sargassum berhubungan erat dengan total fenol. Kelima spesies rumput laut Sargassum di Perairan Simeulue mempunyai aktivitas antioksidan yang kuat (74,7-152,4 μg/mL) sehingga berpotensi dikembangkan sebagai antioksidan.

English

Sargassum sp. is a type of seaweed that proliferates naturally in the waters of Indonesia, such as those surrounding Simeulue in the Aceh Province. Sargassum comprises a range of bioactive compounds, including phenols, that exhibit antioxidant properties. The utilization of Sargassum sp. in the waters of Simeulue by the local community has not been documented, and there are no scientific reports available on the total phenol content and antioxidant activity of this particular seaweed. The primary objective of this study was to investigate the antioxidant properties and total phenol content of Sargassum sp., a seaweed species found in the water of Simeulue. The research methodology encompassed seaweed sampling, followed by the preparation and extraction of seaweed samples, phytochemical testing, and the determination of total phenol content and antioxidant activity of the seaweed samples. The analysis of the relationship between total phenol content and DPPH antioxidant activity of Sargassum was conducted using the linear regression technique. The study findings indicate that the levels of total phenols and antioxidant activity in the seaweed genus Sargassum (S. muticum, S. binderi, S. crassifolium, S. granuliferum, and S. fluitans) collected from Simeulue waters exhibited diverse results. Total phenolics in the sample ranged from 7.45-9.02 mg GAE/g, with the highest amount found in S. binderi and the lowest in S. fluitans. Similarly, antioxidant activity varied from 74.7-152.4 μg/mL, and the highest levels were observed in S. binderi, while the lowest were found in S. fluitans. Correlation analysis (R2 = 0.9059) indicated that the antioxidant activity of the seaweed genus Sargassum was strongly associated with the total phenol content. The findings of this research indicate that the five varieties of Sargassum seaweed present in the waters of Simeulue exhibit considerable antioxidant activity (74.7-152.4 μg/mL), suggesting their potential use as antioxidants.

Keywords

• bioactive compound
• Sargassum
• seaweed
• Simeulue
• rumput laut
• senyawa bioaktif