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Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene

Tropical Animal Science Journal · Vol. 41 No. 1 (2018) · pp. 1–7

DOI
10.5398/tasj.2018.41.1.1
ISSN
2615-790X, 2615-787X
Published
2018-04-06
Publisher
Faculty of Animal Science, IPB University
Language
eng

Abstract

Sperms are vulnerable to oxidative stress due to the high proportion of polyunsaturated fatty acids within their membranes. This condition could decrease sperms quality during preservation. β-carotene is among antioxidants able to neutralize reactive oxygen species, natural by-products of oxygen metabolism in sperms. This study was done to investigate the capability and protective effect of this antioxidant on motility, viability, acrosomal integrity, and plasma membrane integrity of frozen-thawed sperms of Aceh swamp buffalo. The study was conducted using completely randomized design with four treatments and five replications. Fresh semen was diluted in egg-yolk tris-based extenders with the addition of antioxidant of 0% (w/v) (control, P0), 0.625% (w/v) (P1), 1.25% (w/v) (P2), and 2.5% (w/v) (P3) prior to freezing. Variables of sperms quality examined were motility, viability, acrosomal integrity, and plasma membrane integrity of semen after dilution, equilibration, and thawing. Semen having the best post-thawing quality after β-carotene treatment was used for artificial insemination (AI) for accessing its pregnancy rate. The results showed that the addition of 0.625% (w/v) β-carotene (P1) resulted in the highest motility, viability, acrosomal integrity, and plasma membrane integrity. Pregnancy rate of buffalo inseminated with semen treated with 0.625% β-carotene (P1) was 50%. In conclusion, administration of 0.625% β-carotene was able to maintain and could protect motility, viability, acrosomal integrity, and plasma membrane integrity of frozen sperms of Aceh swamp buffalo that are good for artificial insemination.

Keywords

Collaboration network of the authors

40 authors · 131 co-authorship links · showing the 40 most connected

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  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
H. MAHESHWARIAffiliation unknown3
HERDISAffiliation unknown2
Rahmat HidayatUniversitas Halu Oleo2
I. G. PermanaAffiliation unknown2
T. ToharmatAffiliation unknown2
N. M. D. HaqAffiliation unknown2
AmroziAffiliation unknown2
. YulnawatiAffiliation unknown2
Holijah HolijahAffiliation unknown1
YULNAWATI YULNAWATIAffiliation unknown1
M.A SetiadiAffiliation unknown1
I SupriatnaAffiliation unknown1
M ImronAffiliation unknown1
E.T MergawatiAffiliation unknown1
E.M KaiinAffiliation unknown1
N.D YanthiAffiliation unknown1
Indriawati .Affiliation unknown1
A.P. SinuratAffiliation unknown1
T. PurwadariaAffiliation unknown1
P.P KetarenAffiliation unknown1
I.A.K BintangAffiliation unknown1
N. BermawieAffiliation unknown1
M. RaharjoAffiliation unknown1
WahyudinAffiliation unknown1
R. WidyastutiAffiliation unknown1
C. SumantriAffiliation unknown1
D. N. PristihadiAffiliation unknown1
W ManaluAffiliation unknown1
A WinartoAffiliation unknown1
K. B. PutroAffiliation unknown1
B PurwantaraAffiliation unknown1
M AgilAffiliation unknown1
W. P. SuryadiAffiliation unknown1
L. Nur’uscelawatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Quality Enhancement of Aceh Swamp Buffalo (Bubalus bubalis) Frozen Semen by Supplementing β-Carotene</dc:title>
	<dc:creator xml:lang="en">Eriani, K.</dc:creator>
	<dc:creator xml:lang="en">Azhar, A.</dc:creator>
	<dc:creator xml:lang="en">Ihdina, M.</dc:creator>
	<dc:creator xml:lang="en">Rosadi, B.</dc:creator>
	<dc:creator xml:lang="en">Rizal, M.</dc:creator>
	<dc:creator xml:lang="en">Boediono, A.</dc:creator>
	<dc:subject xml:lang="en">Aceh swamp buffalo</dc:subject>
	<dc:subject xml:lang="en">frozen semen</dc:subject>
	<dc:subject xml:lang="en">antioxidant</dc:subject>
	<dc:subject xml:lang="en">β-carotene</dc:subject>
	<dc:subject xml:lang="en">thawing</dc:subject>
	<dc:description xml:lang="en">Sperms are vulnerable to oxidative stress due to the high proportion of polyunsaturated fatty acids within their membranes. This condition could decrease sperms quality during preservation. β-carotene is among antioxidants able to neutralize reactive oxygen species, natural by-products of oxygen metabolism in sperms. This study was done to investigate the capability and protective effect of this antioxidant on motility, viability, acrosomal integrity, and plasma membrane integrity of frozen-thawed sperms of Aceh swamp buffalo. The study was conducted using completely randomized design with four treatments and five replications. Fresh semen was diluted in egg-yolk tris-based extenders with the addition of antioxidant of 0% (w/v) (control, P0), 0.625% (w/v) (P1), 1.25% (w/v) (P2), and 2.5% (w/v) (P3) prior to freezing. Variables of sperms quality examined were  motility, viability, acrosomal integrity, and plasma membrane integrity of semen after dilution, equilibration, and thawing. Semen having the best post-thawing quality after β-carotene treatment was used for artificial insemination (AI) for accessing its pregnancy rate. The results showed that the addition of 0.625% (w/v) β-carotene (P1) resulted in the highest motility, viability, acrosomal integrity, and plasma membrane integrity. Pregnancy rate of buffalo inseminated with semen treated with 0.625% β-carotene (P1) was 50%. In conclusion, administration of 0.625% β-carotene was able to maintain and could protect motility, viability, acrosomal integrity, and plasma membrane integrity of frozen sperms of Aceh swamp buffalo that are good for artificial insemination.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2018-04-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/20944</dc:identifier>
	<dc:identifier>10.5398/tasj.2018.41.1.1</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 41 No. 1 (2018): Tropical Animal Science Journal; 1-7</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/20944/14336</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>