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Isolation of Cellulolytic Lactic-Acid Bacteria from Mentok (Anas moschata) Gastro-Intestinal Tract

Tropical Animal Science Journal · Vol. 41 No. 3 (2018) · pp. 200–206

DOI
10.5398/tasj.2018.41.3.200
ISSN
2615-790X, 2615-787X
Published
2018-11-30
Publisher
Faculty of Animal Science, IPB University
Language
eng

Abstract

Mentok, a local Indonesian name’s of muscovy duck (Anas moschata), has been known as a duck with the capability to consume poor quality feed, high with non-starch carbohydrate (NSP) content. This capacity occurs because of the presence of microbial fermentation activity in the gastro intestinal tract (GIT) of mentok. However, the information about the identification and characterization of the cellulolytic microbes involved is limited. This study was expected to provide scientific contributions about gastrointestinal microbes, especially Lactic Acid Bacteria (LAB), with cellulolytic activities. The experiment was conducted to select LAB with cellulolytic activity from the GIT of mentok. Twenty six of selected LABs were isolated from the duodenum, cecum, and colon regions after microbiological characterization, i.e., morphology, catalase test, gas production, Gram staining, and motility test. Characterization for cellulolytic activity was analyzed by measuring the clearing zone on Carboxymethylcellulose (CMC) media, cell growth analysis on 1% CMC as a carbon source, and CMCase value. Pediococcus acidilactici MK 20 isolate from colon region was selected LAB with the highest cellulolytic activity with the clearing zone diameter, and the CMCase value of 2.33 mm and 0.0153 U/mL, respectively. Molecular identification using 16S rRNA gene sequences analysis revealed that P. acidilactici MK 20 isolate has 99% similarity with P. acidilactici strain ZW001. It was concluded that P. acidilactici MK 20 isolated from the colon part of the gastrointestinal tract of mentok, the Indonesian muscovy duck (A. moschata) had cellulolytic activity.

Keywords

Collaboration network of the authors

40 authors · 115 co-authorship links · 41 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Sebelas Maret University
  • Indonesian Institute of Sciences
  • Universitas Katolik Widya Mandira
  • IPB University
  • National Research and Innovation Agency
  • East Timor Coffee Institute
  • University of Aberdeen
  • Other organisations
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Ahmad SofyanIndonesian Institute of Sciences8
Hardi JulendraIndonesian Institute of Sciences7
Mohammad Faiz KarimyIndonesian Institute of Sciences5
Awistaros Angger SaktiIndonesian Institute of Sciences4
Artini PangastutiSebelas Maret University3
Andi FebrisiantosaAffiliation unknown2
Hildegardis MissaUniversitas Katolik Widya Mandira1
Sardina NdukangUniversitas Katolik Widya Mandira1
Aloysus DjaloUniversitas Katolik Widya Mandira1
Getrudis Wilhelmina NauUniversitas Katolik Widya Mandira1
Anselmus Boy BaunseleUniversitas Katolik Widya Mandira1
Americo Dos SantosEast Timor Coffee Institute1
Justin M. J. TravisUniversity of Aberdeen1
Wilsen ChuanataSebelas Maret University1
Agung BudiharjoSebelas Maret University1
Wardah Radhwa HafizhahSebelas Maret University1
Muhammad AliAbasyn University1
Ratna SetyaningsihSebelas Maret University1
Farah AldilaSebelas Maret University1
Aris Tri WahyudiIPB University1
Suryo WiyonoIPB University1
Antonius SuwantoIPB University1
Yulin LestariIPB University1
Sajidan SajidanSebelas Maret University1
Baraka Stewart MkumbeSebelas Maret University1
Ralf GreinerMax Rubner Institut1
- MustofaAffiliation unknown1
Rofiq SunaryantoBadan Pengkajian dan Penerapan Teknologi1
Heru Agus CahyantoNational Research and Innovation Agency1
Lies Mira YusiatiUniversitas Gadjah Mada1
Achmad DinotoNational Research and Innovation Agency1
Sandy Samsul BahrySebelas Maret University1
Tri UntariAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Isolation of Cellulolytic Lactic-Acid Bacteria from Mentok (Anas moschata)  Gastro-Intestinal Tract</dc:title>
	<dc:creator xml:lang="en">Herdian, H.</dc:creator>
	<dc:creator xml:lang="en">Istiqomah, L.</dc:creator>
	<dc:creator xml:lang="en">Damayanti, E.</dc:creator>
	<dc:creator xml:lang="en">Suryani, A. E.</dc:creator>
	<dc:creator xml:lang="en">Anggraeni, A. S.</dc:creator>
	<dc:creator xml:lang="en">Rosyada, N.</dc:creator>
	<dc:creator xml:lang="en">Susilowati, A.</dc:creator>
	<dc:subject xml:lang="en">lactic acid bacteria</dc:subject>
	<dc:subject xml:lang="en">mentok</dc:subject>
	<dc:subject xml:lang="en">muscovy duck (Anas moschata)</dc:subject>
	<dc:subject xml:lang="en">cellulolytic bacteria</dc:subject>
	<dc:description xml:lang="en">Mentok, a local Indonesian name’s of muscovy duck (Anas moschata), has been known as a duck with the capability to consume poor quality feed, high with non-starch carbohydrate (NSP) content. This capacity occurs because of the presence of microbial fermentation activity in the gastro intestinal tract (GIT) of mentok. However, the information about the identification and characterization of the cellulolytic microbes involved is limited. This study was expected to provide scientific contributions about gastrointestinal microbes, especially Lactic Acid Bacteria (LAB), with cellulolytic activities. The experiment was conducted to select LAB with cellulolytic activity from the GIT of mentok. Twenty six of selected LABs were isolated from the duodenum, cecum, and colon regions after microbiological characterization, i.e., morphology, catalase test, gas production, Gram staining, and motility test. Characterization for cellulolytic activity was analyzed by measuring the clearing zone on Carboxymethylcellulose (CMC) media, cell growth analysis on 1% CMC as a carbon source, and CMCase value. Pediococcus acidilactici MK 20 isolate from colon region was selected LAB with the highest cellulolytic activity with the clearing zone diameter, and the CMCase value of 2.33 mm and 0.0153 U/mL, respectively. Molecular identification using 16S rRNA gene sequences analysis revealed that P. acidilactici MK 20 isolate has 99% similarity with P. acidilactici strain ZW001. It was concluded that P. acidilactici MK 20 isolated from the colon part of the  gastrointestinal tract of mentok, the Indonesian muscovy duck (A. moschata) had cellulolytic activity.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2018-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/21190</dc:identifier>
	<dc:identifier>10.5398/tasj.2018.41.3.200</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 41 No. 3 (2018): Tropical Animal Science Journal; 200-206</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/21190/15898</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Tropical Animal Science Journal</dc:rights>
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