Melola Discovery

← Melola Discovery

Journal SINTA 1

In Vitro and in Vivo Anthelmintic Activities of Aqueous Leaf Infusion of Azadirachta indica against Haemonchus contortus

Tropical Animal Science Journal · Vol. 41 No. 3 (2018) · pp. 185–190

DOI
10.5398/tasj.2018.41.3.185
ISSN
2615-790X, 2615-787X
Published
2018-11-30
Publisher
Faculty of Animal Science, IPB University
Language
eng

Abstract

Anthelmintic resistance of Haemonchus contortus worm has become a major problem in ruminant production. Traditional medicinal and potential tropical plants with high tannin contents have a great potency as an alternative source of bio-anthelmintic. The study was carried out to assess the in vitro egg hatches inhibiting (EHI) and adult worm motility (AWM) tests and in vivo anthelmintic activities of aqueous leaf infusion of Azadirachta indica against H. contortus. Leaf infusion at doses of 2%, 4%, and 6% were used for in vitro treatments, and albendazole at a dose of 2 mg/mL was used as a positive control and 0.9% of sodium chloride was used as a negative control. The EHI assay was conducted two times i.e., before treatment and 24 h after treatment. The AWM were monitored 15 and 30 min, and 1, 2, 3, 4, 5, 6, 7, and 8 h post treatment. Sixteen Javanese Thin Tail ewes at the ages of ±12 months that naturally positive of H. contortus with about 1.000 EPG were selected and allocated randomly to 4 groups (n= 4). They were subsequently received the ensuing treatments i.e., A. indica infusion at doses of 0% (A) as a control, 6% (B), 8% (C), and 5 mg/BW albendazole (D) at a single oral dose as a positive control. Experimental ewes in Groups B and C were given oral infusion weekly during 4 weeks of treatment. EPG’s fecal examinations were conducted weekly. The result showed that the leaf infusion of A. indica containing condensed tannin (CT) at a dose of 6% significantly inhibited egg hatching (P<0.05) with a better effect compared to doses of 0%, 2%, and 4%. In AWM test, three doses of infusion significantly difference each other, as well as both negative and positive controls (P<0.05). After treatment, both of the A. indica containing CT levels and albendazole reduced EPG count significantly (P<0.05). Meanwhile, significant difference was not detected in dose of 8% aqueous leaf infusion of A. indica and albendazole treatments on weeks 3, 4, 5, and 6 consecutively. In conclusion, the aqueous leaf infusion of A. indica is fully potential as a bio anthelmintic against H. contortus.

Keywords

Collaboration network of the authors

33 authors · 164 co-authorship links · 1 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Politeknik Negeri Ujung Pandang
  • Universitas Gadjah Mada
  • Cenderawasih University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
A. SofyanAffiliation unknown4
KustantinahAffiliation unknown4
B. SuwignyoAffiliation unknown4
Pramono -Affiliation unknown2
L. HartatiAffiliation unknown2
R. FidriyantoAffiliation unknown2
H. KusnadiAffiliation unknown2
A. PrasetyoAffiliation unknown2
C. PutnarubunAffiliation unknown2
S. PermadiAffiliation unknown2
I. HudaifaAffiliation unknown2
WulandariAffiliation unknown2
PanjonoAffiliation unknown2
Z. A. BaihaqiAffiliation unknown2
M. A. HarahapAffiliation unknown2
PrasetyoAffiliation unknown2
Nur HidayahPoliteknik Negeri Ujung Pandang1
Cuk Tri NoviandiUniversitas Gadjah Mada1
Andriyani AstutiAffiliation unknown1
Pradita Iustitia SitaresmiAffiliation unknown1
Ahimsa Kandi SaririAffiliation unknown1
Zaenal BachruddinAffiliation unknown1
Chusnul HanimAffiliation unknown1
Ahmad SofyanCenderawasih University1
M. F. KarimyAffiliation unknown1
H. JulendraAffiliation unknown1
L. IstiqomahAffiliation unknown1
H. HerdianAffiliation unknown1
E. DamayantiAffiliation unknown1
A. E. SuryaniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">In Vitro and in Vivo Anthelmintic Activities of Aqueous Leaf Infusion of Azadirachta indica against Haemonchus contortus</dc:title>
	<dc:creator xml:lang="en">Sakti, A. A.</dc:creator>
	<dc:creator xml:lang="en">Kustantinah, Kustantinah</dc:creator>
	<dc:creator xml:lang="en">Nurcahyo, R. W.</dc:creator>
	<dc:subject xml:lang="en">Haemonchus contortus</dc:subject>
	<dc:subject xml:lang="en">Azadirachta indica</dc:subject>
	<dc:subject xml:lang="en">anthelmintic activity</dc:subject>
	<dc:subject xml:lang="en">leaf extract</dc:subject>
	<dc:subject xml:lang="en">sheep</dc:subject>
	<dc:description xml:lang="en">Anthelmintic resistance of Haemonchus contortus worm has become a major problem in ruminant production. Traditional medicinal and potential tropical plants with high tannin contents have a great potency as an alternative source of bio-anthelmintic. The study was carried out to assess the in vitro egg hatches inhibiting (EHI) and adult worm motility (AWM) tests and in vivo anthelmintic activities of aqueous leaf infusion of Azadirachta indica against H. contortus. Leaf infusion at doses of 2%, 4%, and 6% were used for in vitro treatments, and albendazole at a dose of 2 mg/mL was used as a positive control and 0.9% of sodium chloride was used as a negative control. The EHI assay was conducted two times i.e., before treatment and 24 h after treatment. The AWM were monitored 15 and 30 min, and 1, 2, 3, 4, 5, 6, 7, and 8 h post treatment. Sixteen Javanese Thin Tail ewes at the ages of ±12 months that naturally positive of H. contortus with about 1.000 EPG were selected and allocated randomly to 4 groups (n= 4). They were subsequently received the ensuing treatments i.e., A. indica infusion at doses of 0% (A) as a control, 6% (B), 8% (C), and 5 mg/BW albendazole (D) at a single oral dose as a positive control. Experimental ewes in Groups B and C were given oral infusion weekly during 4 weeks of treatment. EPG’s fecal examinations were conducted weekly. The result showed that the leaf infusion of A. indica containing condensed tannin (CT) at a dose of 6% significantly inhibited egg hatching (P&amp;lt;0.05) with a better effect compared to doses of 0%, 2%, and 4%. In AWM test, three doses of infusion significantly difference each other, as well as both negative and positive controls (P&amp;lt;0.05). After treatment, both of the A. indica containing CT levels and albendazole reduced EPG count significantly (P&amp;lt;0.05). Meanwhile, significant difference was not detected in dose of 8% aqueous leaf infusion of A. indica and albendazole treatments on weeks 3, 4, 5, and 6 consecutively. In conclusion, the aqueous leaf infusion of A. indica is fully potential as a bio anthelmintic against H. contortus.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2018-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/21414</dc:identifier>
	<dc:identifier>10.5398/tasj.2018.41.3.185</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 41 No. 3 (2018): Tropical Animal Science Journal; 185-190</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/21414/15895</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2018 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>