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Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken

Tropical Animal Science Journal · Vol. 43 No. 3 (2020) · pp. 197–204

DOI
10.5398/tasj.2020.43.3.197
ISSN
2615-790X, 2615-787X
Published
2020-09-02
Publisher
Faculty of Animal Science, IPB University
Language
eng

Abstract

The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G>A, c.562 T>A, c.588 A>G, and c.612 C>G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G>A and c.562 T>A SNPs were in Hardy Weinberg’s equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A>G and c.612 C>G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e., haplotypes 1, 2, 3, 4, 5, 6, 7, and 8. Haplotypes 1, 2, and 8 had high frequencies (17.6%-23.5%). The c.588 A>G and c.612 C>G mutations were significantly associated (p A were significantly associated (p G and c.612 C>G mutations had higher IgY concentration and ND antibody titers (p<0.05) compared to the other haplotypes. In conclusion, this study has identified the CD1B gene as a polymorphic and is associated with IgY concentration and ND antibody titers in IPB-D1 chicken.

Keywords

Collaboration network of the authors

40 authors · 129 co-authorship links · showing the 40 most connected

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  • Affiliation unknown
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Co-authorOrganisationJoint articles
D. LestariAffiliation unknown11
R.R. NoorAffiliation unknown8
K. ListyariniAffiliation unknown8
A AnggraeniAffiliation unknown6
Jakaria JakariaIPB University6
A FarajallahAffiliation unknown4
I InounuAffiliation unknown4
T SartikaAffiliation unknown4
R.I ArifiantiniAffiliation unknown4
I SupriatnaAffiliation unknown3
F SaputraAffiliation unknown3
. JakariaAffiliation unknown3
M YaminAffiliation unknown3
R.H. MulyonoAffiliation unknown3
D D SolihinAffiliation unknown3
M. RusdinAffiliation unknown3
C BudimanAffiliation unknown3
M. F. UlumAffiliation unknown3
E AndreasAffiliation unknown2
L. PraharaniAffiliation unknown2
SubandriyoAffiliation unknown2
Eko HandiwirawanAffiliation unknown2
E.D GunardiAffiliation unknown2
A.B.L. IshakAffiliation unknown2
Anneke AnggraeniAffiliation unknown2
H. MAHESHWARIAffiliation unknown2
Muladno MuladnoIPB University1
M. A. PagalaAffiliation unknown1
C. SumatriAffiliation unknown1
L. HendraningsihAffiliation unknown1
J. AhmadiAffiliation unknown1
A. MuktianiAffiliation unknown1
B. SulistyantoAffiliation unknown1
Hartati. .Affiliation unknown1
. MuladnoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken</dc:title>
	<dc:creator xml:lang="en">Al-Habib, M. F.</dc:creator>
	<dc:creator xml:lang="en">Murtini, S.</dc:creator>
	<dc:creator xml:lang="en">Gunawan, A.</dc:creator>
	<dc:creator xml:lang="en">Ulupi, N.</dc:creator>
	<dc:creator xml:lang="en">Sumantri, C.</dc:creator>
	<dc:subject xml:lang="en">IPB-D1 chicken</dc:subject>
	<dc:subject xml:lang="en">CD1B</dc:subject>
	<dc:subject xml:lang="en">immunity</dc:subject>
	<dc:subject xml:lang="en">yolk immunoglobulin</dc:subject>
	<dc:subject xml:lang="en">ND antibody titer</dc:subject>
	<dc:description xml:lang="en">The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G&amp;gt;A, c.562 T&amp;gt;A, c.588 A&amp;gt;G, and c.612 C&amp;gt;G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G&amp;gt;A and c.562 T&amp;gt;A SNPs were in Hardy Weinberg’s equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A&amp;gt;G and c.612 C&amp;gt;G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e., haplotypes 1, 2, 3, 4, 5, 6, 7, and 8. Haplotypes 1, 2, and 8 had high frequencies (17.6%-23.5%). The c.588 A&amp;gt;G and c.612 C&amp;gt;G mutations were significantly associated (p&amp;lt;0.05) with IgY concentration and c.562 T&amp;gt;A were significantly associated (p&amp;lt;0.05) with ND antibody titers. The haplotypes 2 and 8 with a combination of c.588 A&amp;gt;G and c.612 C&amp;gt;G mutations had higher IgY concentration and ND antibody titers (p&amp;lt;0.05) compared to the other haplotypes. In conclusion, this study has identified the CD1B gene as a polymorphic and is associated with IgY concentration and ND antibody titers in IPB-D1 chicken.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2020-09-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/29087</dc:identifier>
	<dc:identifier>10.5398/tasj.2020.43.3.197</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 43 No. 3 (2020): Tropical Animal Science Journal; 197-204</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2020.43.3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/29087/20370</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2020 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>