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The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance

Tropical Animal Science Journal · Vol. 45 No. 4 (2022) · pp. 429–435

DOI
10.5398/tasj.2022.45.4.429
ISSN
2615-790X, 2615-787X
Published
2022-11-28
Publisher
Faculty of Animal Science, IPB University
Language
eng

Abstract

Heat shock protein plays an essential role in thermoregulatory during heat stress responses. This study aims to determine the association of single nucleotide polymorphism (SNP) -69T>G in the promoter region of the heat shock protein 70 member 1A (HSPA1A) gene on heat tolerance in Bali cattle. One hundred and sixteen heads of Bali cattle were collected from different locations such as Pangyangan, Bali Island; Serading, Sumbawa Island; and Sembalun, Lombok Island. The SNP was analyzed by genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), which used BstUI enzyme restriction. Physiological responses including respiration rate (Rr), rectal temperature (Tr), heart rate (Hr), heat tolerance coefficient (HTC), and blood glucose level (Glu) were measured. Association analysis was conducted using a general linear model by setting genotype, altitude, and sex as factors. The SNP -69T>G variant of HSPA1A gene found in this study were wild type (TT) with 144 bp & 498 bp; GG with 144, 236, & 262 bp; and TG with 144, 236, 262, & 498 bp. Bali cattle with the GG genotype had lower (p G HSPA1A was associated with the physiological performances of Bali cattle. SNP -69T>G of HSPA1A could be utilized for candidate marker-assisted selection of Bali cattle to improve the performance of heat tolerance.

Keywords

Collaboration network of the authors

40 authors · 118 co-authorship links · showing the 40 most connected

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  • IPB University
  • Universiti Teknikal Malaysia Melaka
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
C. SumantriAffiliation unknown7
I SupriatnaAffiliation unknown3
Jakaria JakariaIPB University3
A. GunawanAffiliation unknown3
K. G. WiryawanAffiliation unknown2
A. ParakkasiAffiliation unknown2
SubandriyoAffiliation unknown2
P.S HardjosworoAffiliation unknown2
E.D GunardiAffiliation unknown2
B. TiesnamurtiAffiliation unknown2
S SaidAffiliation unknown2
T. N. RalahaluAffiliation unknown1
. KartiarsoAffiliation unknown1
Chalid TalibAffiliation unknown1
Hartati. .Affiliation unknown1
. MuladnoAffiliation unknown1
. JakariaAffiliation unknown1
. AryogiAffiliation unknown1
Mokhamad Fakhrul UlumIPB University1
Ronny Rachman NoorAffiliation unknown1
Nadira Andarini SiregarAffiliation unknown1
Bambang Ngaji UtomoAffiliation unknown1
E.D. GurnadiAffiliation unknown1
L. AbdullahUniversiti Teknikal Malaysia Melaka1
Wasmen ManaluAffiliation unknown1
Edit Lesa AditiaAffiliation unknown1
Panca Dewi Maha Rani KartiAffiliation unknown1
I InounuAffiliation unknown1
S SukmawatiAffiliation unknown1
RADEN IIS ARIFIANTINIAffiliation unknown1
Gunawan SitanggangAffiliation unknown1
I.P NandaAffiliation unknown1
Ismeth InounuAffiliation unknown1
D MauluddinAffiliation unknown1
P.R. MatitaputtyAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">The Association of Single Nucleotide Polymorphism -69T&gt;G HSPA1A Gene with Bali Cattle Heat Tolerance</dc:title>
	<dc:creator xml:lang="en">Suhendro, I.</dc:creator>
	<dc:creator xml:lang="en">Jakaria, J.</dc:creator>
	<dc:creator xml:lang="en">Priyanto, R.</dc:creator>
	<dc:creator xml:lang="en">Manalu, W.</dc:creator>
	<dc:creator xml:lang="en">Noor, R. R.</dc:creator>
	<dc:subject xml:lang="en">Bali cattle</dc:subject>
	<dc:subject xml:lang="en">heat tolerance</dc:subject>
	<dc:subject xml:lang="en">HSPA1A gene</dc:subject>
	<dc:subject xml:lang="en">promoter region</dc:subject>
	<dc:subject xml:lang="en">SNP</dc:subject>
	<dc:description xml:lang="en">Heat shock protein plays an essential role in thermoregulatory during heat stress responses. This study aims to determine the association of single nucleotide polymorphism (SNP) -69T&amp;gt;G in the promoter region of the heat shock protein 70 member 1A (HSPA1A) gene on heat tolerance in Bali cattle. One hundred and sixteen heads of Bali cattle were collected from different locations such as Pangyangan, Bali Island; Serading, Sumbawa Island; and Sembalun, Lombok Island. The SNP was analyzed by genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), which used BstUI enzyme restriction. Physiological responses including respiration rate (Rr), rectal temperature (Tr), heart rate (Hr), heat tolerance coefficient (HTC), and blood glucose level (Glu) were measured. Association analysis was conducted using a general linear model by setting genotype, altitude, and sex as factors. The SNP -69T&amp;gt;G variant of HSPA1A gene found in this study were wild type (TT) with 144 bp &amp;amp; 498 bp; GG with 144, 236, &amp;amp; 262 bp; and TG with 144, 236, 262, &amp;amp; 498 bp. Bali cattle with the GG genotype had lower (p&amp;lt;0.001) Rr and HTC compared to the other genotypes. It could be concluded that physiological performances were lower at high altitudes, and the SNP -69T&amp;gt;G HSPA1A was associated with the physiological performances of Bali cattle. SNP -69T&amp;gt;G of HSPA1A could be utilized for candidate marker-assisted selection of Bali cattle to improve the performance of heat tolerance.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2022-11-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/39990</dc:identifier>
	<dc:identifier>10.5398/tasj.2022.45.4.429</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 45 No. 4 (2022): Tropical Animal Science Journal; 429-435</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2022.45.4</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/39990/24456</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>