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Quantitative Histomorphometry of Pre-ovulatory Follicles and Uterine Glands of Ongole-grade Heifer in Response to the Low Doses of PMSG Administration

Tropical Animal Science Journal · Vol. 46 No. 1 (2023) · pp. 1–12

DOI
10.5398/tasj.2023.46.1.1
ISSN
2615-790X, 2615-787X
Published
2023-03-02
Publisher
Faculty of Animal Science, IPB University
Language
eng

Abstract

Injection of female mammalian animals with gonadotropin prior to mating using superovulation dose increases estradiol 17beta (E2) synthesis and secretion and improves the growth and development of the uterus to support prenatal growth with the side effect of increasing litter size. This experiment was designed to study the effects of low doses of PMSG injection in heifers on the growth of granulose cells (GC) of pre-ovulatory follicles, E2 synthesis, and uterine glands growth and development. Nine Ongole-grade heifers were divided into three groups of doses of PMSG injection, i.e., 0 IU/kg BW, 0.5 IU/kg BW, and 1.0 IU/kg BW. The injection of pregnant mare serum gonadotropin (PMSG) was conducted at the early stage of the second follicle wave. Blood samples were collected at estrus (day 0) to measure plasma E2 concentrations. The ovary and uterus were collected for histological observation after slaughtering the animal. The results showed that non-superovulation doses of PMSG injections at the second follicle wave significantly increased the growth of GC that eventually produced a significantly higher plasma E2 concentration by 39.47% and 113.57%, in heifers receiving 0.5 IU/kg BW and 1.0 IU/kg BW (p<0.05), respectively. The increase in E2 synthesis and secretion significantly stimulated the growth and development of uterine glands (p<0.05), which was reflected in the improved measures of uterine glands. In conclusion, injections of heifers with low doses of PMSG at the second follicle wave could increase the growth of GC to produce a higher secretion of E2 that eventually improves the growth and development of uterine glands in the endometrium without the risk of multiple calving.

Keywords

Collaboration network of the authors

40 authors · 98 co-authorship links · showing the 40 most connected

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  • IPB University
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
B PurwantaraAffiliation unknown5
R. PriyantoAffiliation unknown3
I SupriatnaAffiliation unknown3
N. M. D. HaqAffiliation unknown3
Riasari Gail SianturiAffiliation unknown2
P SitumorangAffiliation unknown2
Rahmat HidayatUniversitas Halu Oleo2
I. G. PermanaAffiliation unknown2
T. ToharmatAffiliation unknown2
R.R. NoorAffiliation unknown2
J JakariaAffiliation unknown2
I. SuhendroAffiliation unknown2
H. MAHESHWARIAffiliation unknown2
Sony Hartono WijayaIPB University1
Asep SudarmanAffiliation unknown1
Siti Nurma IndahAffiliation unknown1
Dilla Mareistia FassahAffiliation unknown1
. YulnawatiAffiliation unknown1
M.A SetiadiAffiliation unknown1
M ImronAffiliation unknown1
Mohamad Agus SetiadiAffiliation unknown1
Muhammad ImronAffiliation unknown1
Iman SupriatnaAffiliation unknown1
Ekowati HandharyaniIPB University1
K OchiaiAffiliation unknown1
SugitoAffiliation unknown1
ChairulAffiliation unknown1
E HandharyaniAffiliation unknown1
. ZubirAffiliation unknown1
H.M. WinugrohoAffiliation unknown1
E. GurnadiAffiliation unknown1
K ErianiAffiliation unknown1
A. AzharAffiliation unknown1
M. IhdinaAffiliation unknown1
B. RosadiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.ipb.ac.id/index.php/tasj/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Quantitative Histomorphometry of Pre-ovulatory Follicles and Uterine Glands of Ongole-grade Heifer in Response to the Low Doses of PMSG Administration</dc:title>
	<dc:creator xml:lang="en">Putro, K. B.</dc:creator>
	<dc:creator xml:lang="en">Winarto, A.</dc:creator>
	<dc:creator xml:lang="en">Amrozi</dc:creator>
	<dc:creator xml:lang="en">Boediono, A.</dc:creator>
	<dc:creator xml:lang="en">Manalu, W.</dc:creator>
	<dc:subject xml:lang="en">E2</dc:subject>
	<dc:subject xml:lang="en">GC</dc:subject>
	<dc:subject xml:lang="en">Heifer</dc:subject>
	<dc:subject xml:lang="en">pre-ovulatory follicle</dc:subject>
	<dc:subject xml:lang="en">uterine gland</dc:subject>
	<dc:description xml:lang="en">Injection of female mammalian animals with gonadotropin prior to mating using superovulation dose increases estradiol 17beta (E2) synthesis and secretion and improves the growth and development of the uterus to support prenatal growth with the side effect of increasing litter size. This experiment was designed to study the effects of low doses of PMSG injection in heifers on the growth of granulose cells (GC) of pre-ovulatory follicles, E2 synthesis, and uterine glands growth and development. Nine Ongole-grade heifers were divided into three groups of doses of PMSG injection, i.e., 0 IU/kg BW, 0.5 IU/kg BW, and 1.0 IU/kg BW. The injection of pregnant mare serum gonadotropin (PMSG) was conducted at the early stage of the second follicle wave. Blood samples were collected at estrus (day 0) to measure plasma E2 concentrations. The ovary and uterus were collected for histological observation after slaughtering the animal. The results showed that non-superovulation doses of PMSG injections at the second follicle wave significantly increased the growth of GC that eventually produced a significantly higher plasma E2 concentration by 39.47% and 113.57%, in heifers receiving 0.5 IU/kg BW and 1.0 IU/kg BW (p&amp;lt;0.05), respectively. The increase in E2 synthesis and secretion significantly stimulated the growth and development of uterine glands (p&amp;lt;0.05), which was reflected in the improved measures of uterine glands. In conclusion, injections of heifers with low doses of PMSG at the second follicle wave could increase the growth of GC to produce a higher secretion of E2 that eventually improves the growth and development of uterine glands in the endometrium without the risk of multiple calving.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, IPB University</dc:publisher>
	<dc:date>2023-03-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ipb.ac.id/tasj/article/view/40648</dc:identifier>
	<dc:identifier>10.5398/tasj.2023.46.1.1</dc:identifier>
	<dc:source xml:lang="en">Tropical Animal Science Journal; Vol. 46 No. 1 (2023): Tropical Animal Science Journal; 1-12</dc:source>
	<dc:source>2615-790X</dc:source>
	<dc:source>2615-787X</dc:source>
	<dc:source>10.5398/tasj.2023.46.1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ipb.ac.id/tasj/article/view/40648/24963</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2023 Tropical Animal Science Journal</dc:rights>
</oai_dc:dc>