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Zinc supplementation decreases basophilic stippling in rats exposed to lead

Universa Medicina · Vol. 33 No. 1 (2014) · pp. 11–18

DOI
10.18051/UnivMed.2014.v33.11-18
ISSN
2407-2230, 1907-3062
Published
2014-04-19
Publisher
Faculty of Medicine, Universitas Trisakti
Language
eng

Abstract

BACKGROUND Lead acetate inhibits pyrimidine-5’-nucleotidase resulting in ribonucleic acid and ribosome accumulation in erythrocytes (RBC), visible as basophilic stippling (BS). Lead exposure disrupts RBC membrane, shortens the RBC life span and decreases hematocrit. Zinc supplementation increases lead-binding proteins (metallothioneins). The study objective was to determine whether zinc supplementation prior to lead exposure decreases BS and increases the hematocrit in rats. METHODS The study was a randomized post-test only control-group trial, using 28 rats allocated to one control and 3 intervention groups (Zinc I, Zinc II, Zinc III) receiving 0.2 mg, 0.4 mg, and 0.8 mg of zinc by oral gavage daily for 3 weeks. All groups were then exposed to lead at 0.5 g/kg BW/day by gavage for 10 weeks. On the last day of the 10 weeks BS was determined from Giemsastained blood smears and hematocrit by hematology analyzer. Between-group differences were tested with one-way ANOVA, followed by Bonferroni’s test. RESULTS Mean BS was significantly decreasing 7.93 ± 0.99% in controls, 6.91 ± 1.04%, 4.66 ± 0.79%, and 1.73 ± 0.88%, respectively, in intervention groups (p=0.000). Mean hematocrit was significantly increasing 26.16 ± 3.60% in controls, and 30.33 ± 6.12%, 36.83 ± 3.31%, and 40.00 ± 4.77%, respectively, in intervention groups (p=0.000). One-way Anova and Bonferroni’s test showed significant differences in BS and hematocrit between controls and intervention groups receiving zinc supplementation of 0.4 and 0.8 mg (p=0.000). CONCLUSION Zinc supplementation before lead exposure significantly decreases basophilic stippling and increases hematocrit level in rats exposed to lead

Keywords

Collaboration network of the authors

40 authors · 109 co-authorship links · 7 across different organisations · showing the 40 most connected

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  • Diponegoro University
  • Omdurman Islamic University
  • Universitas Sembilanbelas November Kolaka
  • King Faisal University
  • Soegijapranata Catholic University
  • University of Pembangunan Nasional Veteran Jawa Timur
  • Universitas Islam Negeri Sultan Amai Gorontalo
  • Other organisations
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Amar Sharaf Eldin KhairOmdurman Islamic University2
A. H. I. ElfaigAffiliation unknown2
Purwanto PurwantoDiponegoro University2
Suparman SuparmanUniversitas Sembilanbelas November Kolaka2
Fitriani Nur DamayantiAffiliation unknown2
M. E. YassenKing Faisal University1
Lina HerlinaDiponegoro University1
Budi WidianarkoSoegijapranata Catholic University1
Sugiarto SugiartoUniversity of Pembangunan Nasional Veteran Jawa Timur1
Said JunaidiAffiliation unknown1
Khairuddin KhairuddinUniversitas Islam Negeri Sultan Amai Gorontalo1
Aryu CandraAffiliation unknown1
Ika Swasti MahargyaniAffiliation unknown1
Enny ProbosariAffiliation unknown1
Damai SantosaAffiliation unknown1
Yusuf AminullahAffiliation unknown1
Zulfikar NaftaliAffiliation unknown1
Mahmudah MahmudahCenderawasih University1
Maria MexitaliaAffiliation unknown1
Agustini UtariAffiliation unknown1
Magdalena SidhartaniAffiliation unknown1
Moh Syarofil AnamAffiliation unknown1
Ferdy K CayamiAffiliation unknown1
Ariyani LutfitasariAffiliation unknown1
Fitriani Nur DamayantiAffiliation unknown1
Lia MulyantiAffiliation unknown1
Umi KhasanahAffiliation unknown1
Umi CahyaningsihAffiliation unknown1
Arifin Budiman NugrahaAffiliation unknown1
Fadjar SatrijaAffiliation unknown1
Yulia YellitaAffiliation unknown1
Eliyus PutraAffiliation unknown1
RozaAffiliation unknown1
Ridi ArifAffiliation unknown1
Dwi InarsihAffiliation unknown1
Budi SantosoUniversity of Mataram1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://univmed.org/ejurnal/index.php/medicina/oai, first harvested 10 Nov 2026, last seen 10 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Zinc supplementation decreases basophilic stippling in rats exposed to lead</dc:title>
	<dc:creator xml:lang="en">Santosa, Budi</dc:creator>
	<dc:creator xml:lang="en">Subagio, Hertanto Wahyu</dc:creator>
	<dc:creator xml:lang="en">Suratmo, Lisyani</dc:creator>
	<dc:creator xml:lang="en">Sunoko, Henna Rya</dc:creator>
	<dc:subject xml:lang="en">Zinc</dc:subject>
	<dc:subject xml:lang="en">basophilic stippling</dc:subject>
	<dc:subject xml:lang="en">hematocrit</dc:subject>
	<dc:subject xml:lang="en">rats</dc:subject>
	<dc:description xml:lang="en">BACKGROUND Lead acetate inhibits pyrimidine-5’-nucleotidase resulting in ribonucleic acid and ribosome accumulation in erythrocytes (RBC), visible as basophilic stippling (BS). Lead exposure disrupts RBC membrane, shortens the RBC life span and decreases hematocrit. Zinc supplementation increases lead-binding proteins (metallothioneins). The study objective was to determine whether zinc supplementation prior to lead exposure decreases BS and increases the hematocrit in rats. METHODS The study was a randomized post-test only control-group trial, using 28 rats allocated to one control and 3 intervention groups (Zinc I, Zinc II, Zinc III) receiving 0.2 mg, 0.4 mg, and 0.8 mg of zinc by oral gavage daily for 3 weeks. All groups were then exposed to lead at 0.5 g/kg BW/day by gavage for 10 weeks. On the last day of the 10 weeks BS was determined from Giemsastained blood smears and hematocrit by hematology analyzer. Between-group differences were tested with one-way ANOVA, followed by Bonferroni’s test. RESULTS Mean BS was significantly decreasing 7.93 ± 0.99% in controls, 6.91 ± 1.04%, 4.66 ± 0.79%, and 1.73 ± 0.88%, respectively, in intervention groups (p=0.000). Mean hematocrit was significantly increasing 26.16 ± 3.60% in controls, and 30.33 ± 6.12%, 36.83 ± 3.31%, and 40.00 ± 4.77%, respectively, in intervention groups (p=0.000). One-way Anova and Bonferroni’s test showed significant differences in BS and hematocrit between controls and intervention groups receiving zinc supplementation of 0.4 and 0.8 mg (p=0.000). CONCLUSION Zinc supplementation before lead exposure significantly decreases basophilic stippling and increases hematocrit level in rats exposed to lead</dc:description>
	<dc:publisher xml:lang="en">Faculty of Medicine, Universitas Trisakti</dc:publisher>
	<dc:date>2014-04-19</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://univmed.org/ejurnal/index.php/medicina/article/view/15</dc:identifier>
	<dc:identifier>10.18051/UnivMed.2014.v33.11-18</dc:identifier>
	<dc:source xml:lang="en">Universa Medicina; Vol. 33 No. 1 (2014); 11-18</dc:source>
	<dc:source>2407-2230</dc:source>
	<dc:source>1907-3062</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://univmed.org/ejurnal/index.php/medicina/article/view/15/491</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2015 Budi Santosa, Hertanto Wahyu Subagio, Lisyani Suratmo, Henna Rya Sunoko</dc:rights>
</oai_dc:dc>