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Antioxidant Potential of Organic Fraction of Turi Leaf Extract (Sesbania grandiflora L.) Using DPPH Reagent

MEDULA (JURNAL ILMIAH FAKULTAS KEDOKTERAN UNIVERSITAS HALU OLEO) · Vol. 9 No. 2 (2022) · pp. 78–88

DOI
10.46496/medula.v9i2.25291
ISSN
2443-0218, 2339-1006
Published
2022-05-25
Publisher
Halu Oleo University
Language
eng

Abstract

Background: White turi leaf (Sesbania grandiflora) is a plant from the Leguminosae family that isused as traditional medicine. Purpose(s): To determine the antioxidant potential of white turi leafextract (Sesbania grandiflora L) using DPPH reagent. Methods: White turi leaves (S. grandiflora)were extracted using maceration technique with ethanol the solvent. The ethanol extract wasfractionated using a liquid-liquid extraction using n-hexane, ethyl acetate, and ethanol. In addition,phytochemical screening was also carried out on secundary metabolite compounds contained in theethanol extract, it’s organic fractions. Results: Ethanol extract obtained as much as 160 g (16%).Fractionation of 10 grams of ethanol extract obtained n-hexane fraction 2.9 g (29%); ethyl acetatefraction 0.8 g (8%); and ethanol fraction 6.1 g (61%). Phytochemical screening showed that theethanol extract and ethyl acetate fraction contained flavonoids, saponins, and tannins; the ethanolfraction contains terpenoids, flavonoids, saponins, and tannins; while the n-hexane fraction containstannin compounds. The antioxidant activity of the ethanol extract, ethyl acetate fraction, and ethanolfraction were tested using the DPPH radical scavenging method. The antioxidant activity assayshowed that the SC50 values of the ethanol extract, ethyl acetate fraction, and ethanol fraction were234.7; 66,244, and 106.755 ppm, vitamin C as a positif control with SC50 3,006 ppm. Conclusion:white turi leaf extract (S. grandiflora) has antioxidant activity less than 22-78 times than controlpositif, so it is not potential as an antioxidant.

Collaboration network of the authors

20 authors · 47 co-authorship links · 29 across different organisations

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  • Universitas Halu Oleo
  • Politeknik Bina Husada Kendari
  • Institut Teknologi dan Kesehatan Avicenna
  • Tadulako University
  • Universitas Sembilanbelas November Kolaka
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
W WahyuniUniversitas Halu Oleo2
Fery Indradewi ArmadanyUniversitas Halu Oleo1
Ulfa WildaUniversitas Halu Oleo1
Muhammad Ilyas YUniversitas Halu Oleo1
Haswika HaswikaPoliteknik Bina Husada Kendari1
La Ode Liaumin AzimUniversitas Halu Oleo1
Nur Fitriana Muhammad AliInstitut Teknologi dan Kesehatan Avicenna1
Ikhsan SidiqInstitut Teknologi dan Kesehatan Avicenna1
Andi Nafisah Tendri AdjengUniversitas Halu Oleo1
Rini NuriyantoInstitut Teknologi dan Kesehatan Avicenna1
Fadhliyah MalikUniversitas Halu Oleo1
Yusni Oktaviani TimungUniversitas Halu Oleo1
Carla Wulandari SabandarUniversitas Sembilanbelas November Kolaka1
Muhammad Azdar SetiawanPoliteknik Bina Husada Kendari1
Novita DwisafitriPoliteknik Bina Husada Kendari1
Wa Ode IrmayantiPoliteknik Bina Husada Kendari1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/medula/oai, first harvested 31 Oct 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Antioxidant Potential of Organic Fraction of Turi Leaf Extract (Sesbania grandiflora L.) Using DPPH Reagent</dc:title>
	<dc:creator>Musnina, Wa Ode Sitti</dc:creator>
	<dc:creator>Wulaisfan, Randa</dc:creator>
	<dc:creator>Akhyar, Jumriana</dc:creator>
	<dc:creator>Yuyun, Yonelian</dc:creator>
	<dc:description xml:lang="en-US">Background: White turi leaf (Sesbania grandiflora) is a plant from the Leguminosae family that isused as traditional medicine. Purpose(s): To determine the antioxidant potential of white turi leafextract (Sesbania grandiflora L) using DPPH reagent. Methods: White turi leaves (S. grandiflora)were extracted using maceration technique with ethanol the solvent. The ethanol extract wasfractionated using a liquid-liquid extraction using n-hexane, ethyl acetate, and ethanol. In addition,phytochemical screening was also carried out on secundary metabolite compounds contained in theethanol extract, it’s organic fractions. Results: Ethanol extract obtained as much as 160 g (16%).Fractionation of 10 grams of ethanol extract obtained n-hexane fraction 2.9 g (29%); ethyl acetatefraction 0.8 g (8%); and ethanol fraction 6.1 g (61%). Phytochemical screening showed that theethanol extract and ethyl acetate fraction contained flavonoids, saponins, and tannins; the ethanolfraction contains terpenoids, flavonoids, saponins, and tannins; while the n-hexane fraction containstannin compounds. The antioxidant activity of the ethanol extract, ethyl acetate fraction, and ethanolfraction were tested using the DPPH radical scavenging method. The antioxidant activity assayshowed that the SC50 values of the ethanol extract, ethyl acetate fraction, and ethanol fraction were234.7; 66,244, and 106.755 ppm, vitamin C as a positif control with SC50 3,006 ppm. Conclusion:white turi leaf extract (S. grandiflora) has antioxidant activity less than 22-78 times than controlpositif, so it is not potential as an antioxidant.  </dc:description>
	<dc:publisher xml:lang="en-US">Halu Oleo University</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2022-05-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/medula/article/view/25291</dc:identifier>
	<dc:identifier>10.46496/medula.v9i2.25291</dc:identifier>
	<dc:source xml:lang="en-US">MEDULA (JURNAL ILMIAH FAKULTAS KEDOKTERAN UNIVERSITAS HALU OLEO); Vol 9, No 2 (2022); 78-88</dc:source>
	<dc:source>2443-0218</dc:source>
	<dc:source>2339-1006</dc:source>
	<dc:source>10.46496/medula.v9i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/medula/article/view/25291/pdf</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2022 MEDULA</dc:rights>
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