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Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

JURNAL BIOLOGI PAPUA · Vol. 12 No. 1 (2020) · pp. 43–49

DOI
10.31957/jbp.1096
ISSN
2503-0450, 2086-3314
Published
2020-03-05
Publisher
Jurusan Biologi FMIPA Universitas Cenderawasih
Language
eng

Abstract

The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0%, 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet in vitro.Keywords: Chitosan, orchid, Vacin and Went, plantlet.

Collaboration network of the authors

40 authors · 159 co-authorship links · 4 across different organisations · showing the 40 most connected

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  • Cenderawasih University
  • Universitas Winaya Mukti
  • Universitas Gadjah Mada
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Suharno SuharnoCenderawasih University11
Supeni SufaatiCenderawasih University9
Puguh SujartaCenderawasih University5
Farmawaty FarmawatyCenderawasih University5
Agnes Eri MaryuniCenderawasih University3
Roma M. ManaluCenderawasih University3
Eva Susanty SimaremareCenderawasih University2
Suriani Br. SurbaktiCenderawasih University2
Johnson SiallaganCenderawasih University2
Vita PurnamasariCenderawasih University2
Marseline G. MailisaCenderawasih University2
Irene SuebuCenderawasih University2
Wehelmina LahalloCenderawasih University2
Sri WilujengUniversitas Winaya Mukti1
Rosye H.R. TanjungCenderawasih University1
Suharno SuharnoCenderawasih University1
Suharyanto SuharyantoUniversitas Gadjah Mada1
Chris D. SembayAffiliation unknown1
Lisiard DimaraCenderawasih University1
Elsye GunawanCenderawasih University1
Jane AwomCenderawasih University1
Susan WopiCenderawasih University1
Henderina J. KeiluhuCenderawasih University1
Cheny AdetyaningsihCenderawasih University1
Siti DaryatiCenderawasih University1
Mesak MantekCenderawasih University1
Suwito SuwitoCenderawasih University1
Natalie J. TangkepayungCenderawasih University1
Rahmad S. BudiCenderawasih University1
Linus Yhani ChrystomoCenderawasih University1
Ign. Joko SuyonoCenderawasih University1
Annisa Alfadillah RuhaniAffiliation unknown1
SHERLITA MAULANICenderawasih University1
Alpredo Dias Agape L. ToruanCenderawasih University1
SuharnoCenderawasih University1
SUHARNO SUHARNOCenderawasih University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ejournal.uncen.ac.id/index.php/JBP/oai, first harvested 1 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro</dc:title>
	<dc:creator>Agustini, Verena</dc:creator>
	<dc:creator>Rahayu, Irma</dc:creator>
	<dc:creator>Numberi, Leonardo A.</dc:creator>
	<dc:creator>Ni’mah, Ziyadatun</dc:creator>
	<dc:description xml:lang="en-US">The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm  and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet  in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.</dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Biologi FMIPA Universitas Cenderawasih</dc:publisher>
	<dc:date>2020-03-05</dc:date>
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	<dc:identifier>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1096</dc:identifier>
	<dc:identifier>10.31957/jbp.1096</dc:identifier>
	<dc:source xml:lang="en-US">JURNAL BIOLOGI PAPUA; Vol. 12 No. 1 (2020); 43-49</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biologi Papua; Vol 12 No 1 (2020); 43-49</dc:source>
	<dc:source>2503-0450</dc:source>
	<dc:source>2086-3314</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1096/957</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2020 JURNAL BIOLOGI PAPUA</dc:rights>
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