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Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

JURNAL BIOLOGI PAPUA · Vol. 13 No. 1 (2021) · pp. 28–35

DOI
10.31957/jbp.1299
ISSN
2503-0450, 2086-3314
Published
2021-04-01
Publisher
Jurusan Biologi FMIPA Universitas Cenderawasih
Language
eng

Abstract

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

Collaboration network of the authors

40 authors · 109 co-authorship links · 8 across different organisations · showing the 40 most connected

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  • Cenderawasih University
  • Universitas Gadjah Mada
  • Politeknik Negeri Balikpapan
  • National Research and Innovation Agency
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Puguh SujartaCenderawasih University5
Aditya Krishar KarimCenderawasih University4
Maklon WarpurCenderawasih University2
I Made BudiCenderawasih University2
Nuryani NuryaniAffiliation unknown2
Budi PurnamaAffiliation unknown2
Utari UtariAffiliation unknown2
Riyatun RiyatunAffiliation unknown2
Tomi A. NelamboCenderawasih University2
Sri BananiekAffiliation unknown1
Modustriati P. MardanyCenderawasih University1
Kalep SegenilAffiliation unknown1
Ganis OktaliaCenderawasih University1
Suharyanto SuharyantoUniversitas Gadjah Mada1
Chris D. SembayAffiliation unknown1
Verena AgustiniCenderawasih University1
Lisiard DimaraCenderawasih University1
Ervina IndrayaniCenderawasih University1
Zainal Arifin WasarakaCenderawasih University1
Apianus KilunggaCenderawasih University1
Yuliana H. RumsarwirPoliteknik Negeri Balikpapan1
LISYE IRIANA ZEBUACenderawasih University1
Daniel LantangCenderawasih University1
Ike G. SiregarAffiliation unknown1
Henderina J. KeiluhuCenderawasih University1
SARAH YULIANANational Research and Innovation Agency1
Ronauli SilitongaCenderawasih University1
Ignatius Joko SuyonoAffiliation unknown1
Efray WanimboCenderawasih University1
Maria Ludia SimonapendiCenderawasih University1
Mesak MantekCenderawasih University1
Rahmad S. BudiCenderawasih University1
Suharno SuharnoCenderawasih University1
Ign. Joko SuyonoCenderawasih University1
Irma RahayuCenderawasih University1
Farmawaty FarmawatyCenderawasih University1
Khoiru Rizqy RambeAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ejournal.uncen.ac.id/index.php/JBP/oai, first harvested 1 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin</dc:title>
	<dc:creator>Warpur, Agustriani</dc:creator>
	<dc:creator>Chrystomo, Linus Y.</dc:creator>
	<dc:creator>Suharno, Suharno</dc:creator>
	<dc:description xml:lang="en-US">Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint &amp;amp; Went (V&amp;amp;W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&amp;amp;W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&amp;amp;W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&amp;amp;W medium; auxins; cytokinins.</dc:description>
	<dc:publisher xml:lang="en-US">Jurusan Biologi FMIPA Universitas Cenderawasih</dc:publisher>
	<dc:date>2021-04-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1299</dc:identifier>
	<dc:identifier>10.31957/jbp.1299</dc:identifier>
	<dc:source xml:lang="en-US">JURNAL BIOLOGI PAPUA; Vol. 13 No. 1 (2021); 28-35</dc:source>
	<dc:source xml:lang="id-ID">Jurnal Biologi Papua; Vol 13 No 1 (2021); 28-35</dc:source>
	<dc:source>2503-0450</dc:source>
	<dc:source>2086-3314</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ejournal.uncen.ac.id/index.php/JBP/article/view/1299/1273</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 JURNAL BIOLOGI PAPUA</dc:rights>
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