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Characteristics of Bali Bull (Bos Sondaicus) Sexed Sperms with Freeze Dry Egg White at Different Incubation Time: english

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 4 No. 1 (2022) · pp. 30–40

DOI
10.20956/hajas.v4i1.19259
ISSN
2621-9190, 2621-9182
Published
2022-06-06
Publisher
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

The development of reproductive technology for the separation of spermatozoa with X and Y chromosomes, which is better known as sexing, can be carried out in vitro using various methods, one of which is using freeze dry egg whites. This study aims to determine the quality of semen based on incubation time. This study used four types of treatment, namely P0 = No Incubation, P1 = Incubation 30 minutes, P2 = Incubation 45 minutes, P3 = Incubation 60 minutes with six replications. Parameters observed were the quality of spermatozoa macroscopically (volume, odor, color and consistency) and microscopically (motility, abnormality, concentration, intact plasma membrane, intact acrosome cap). Y using descriptive statistical test. The results of analysis of variance that sexing spermatozoa using freeze dry egg whites at different incubation times showed a significant effect (P 0.05) on concentration, motility, and abnormalities. Evaluation of fresh Bali cattle spermatozoa showed results that were in accordance with the standards for further examination of semen sexing. Incubation time of 30 minutes in the sexing process of spermatozoa caused a significant decrease in motility and concentration, meanwhile, IPM and IAM of sexed spermatozoa experienced a significant decrease in incubation time of 60 minutes, but the decrease was still within the normal range for spermatozoa. to be used in the artificial insemination process. Keywords: Sexing, incubation, concentration, motility, abnormality, intact plasma membrane, intact acrosome caps.

Collaboration network of the authors

32 authors · 79 co-authorship links · 9 across different organisations

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  • Hasanuddin University
  • Universitas Ahmad Dahlan
  • Universitas Halu Oleo
  • Politeknik Pertanian Negeri Pangkajene Kepulauan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Zulkharnaim MaupaAffiliation unknown2
Lellah RahimHasanuddin University2
Sudirman BacoHasanuddin University2
Abdul Latief TolengHasanuddin University2
Muhammad YusufUniversitas Halu Oleo2
Masturi MasturiHasanuddin University2
Toleng Abd LatiefAffiliation unknown2
Nur SalmahHasanuddin University2
Hasrin HasrinHasanuddin University2
Ummul MasirPoliteknik Pertanian Negeri Pangkajene Kepulauan2
Imam RiadiUniversitas Ahmad Dahlan1
Sunardi SunardiUniversitas Ahmad Dahlan1
la ode Maksar MuhurunaAffiliation unknown1
Djoni Prawira RahardjaAffiliation unknown1
Abdul Latif TolengAffiliation unknown1
Rika HaryaniAffiliation unknown1
mawardi A. Asja ZulkarnaimAffiliation unknown1
Muhammad Ihsan Andi DagongAffiliation unknown1
Lellah RahimAffiliation unknown1
Isdam SupriadiAffiliation unknown1
Renny Fatmyah UtamyHasanuddin University1
Nur Eni NurHasanuddin University1
Dina ArdianaHasanuddin University1
Zulkharnaim ZulkharnaimHasanuddin University1
E. DondatuHasanuddin University1
Jasmal Ahmari SyamsuAffiliation unknown1
Rinduwati RinduwatiAffiliation unknown1
Sema SemaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, first harvested 2 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Characteristics of Bali Bull (Bos Sondaicus) Sexed Sperms with Freeze Dry Egg White at Different Incubation Time: english</dc:title>
	<dc:creator xml:lang="en">Damayanti, Putri</dc:creator>
	<dc:creator xml:lang="en">Yusuf, Muhammad</dc:creator>
	<dc:creator xml:lang="en">Ako, Ambo</dc:creator>
	<dc:creator xml:lang="en">Sahiruddin, Sahiruddin</dc:creator>
	<dc:description xml:lang="en">The development of reproductive technology for the separation of spermatozoa with X and Y chromosomes, which is better known as sexing, can be carried out in vitro using various methods, one of which is using freeze dry egg whites. This study aims to determine the quality of semen based on incubation time. This study used four types of treatment, namely P0 = No Incubation, P1 = Incubation 30 minutes, P2 = Incubation 45 minutes, P3 = Incubation 60 minutes with six replications. Parameters observed were the quality of spermatozoa macroscopically (volume, odor, color and consistency) and microscopically (motility, abnormality, concentration, intact plasma membrane, intact acrosome cap). Y using descriptive statistical test. The results of analysis of variance that sexing spermatozoa using freeze dry egg whites at different incubation times showed a significant effect (P&amp;lt;0.05) on intact plasma membrane (IPM), intact acrosomal membrane (IAM), but had no significant effect P&amp;gt;0.05) on concentration, motility, and abnormalities. Evaluation of fresh Bali cattle spermatozoa showed results that were in accordance with the standards for further examination of semen sexing. Incubation time of 30 minutes in the sexing process of spermatozoa caused a significant decrease in motility and concentration, meanwhile, IPM and IAM of sexed spermatozoa experienced a significant decrease in incubation time of 60 minutes, but the decrease was still within the normal range for spermatozoa. to be used in the artificial insemination process.
Keywords:  Sexing, incubation, concentration, motility, abnormality, intact plasma membrane, intact acrosome caps.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2022-06-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/19259</dc:identifier>
	<dc:identifier>10.20956/hajas.v4i1.19259</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 4 No. 1 (2022); 30-40</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/19259/8233</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>