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The Quality of Bali Bull Sexed Sperm Using Soybean Extract Extender

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 4 No. 1 (2022) · pp. 48–57

DOI
10.20956/hajas.v4i1.19439
ISSN
2621-9190, 2621-9182
Published
2022-07-25
Publisher
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

The process of sexing spermatozoa requires a medium that is able to protect and provide an optimal environment. One of them is extender media that is used to extend the volume of semen. The extender that is commonly used is commercially but some limitation such as price and availability. Therefore, it is necessary to have an alternative extender such as soybean. The aim of this study was to know the quality of sexed sperms diluted using soybean extender. Semen of Bali bull was collected for five times and then subjected to three different extender treatments after sexing; T1 (Soybean), T2 (Tris), T3 (Tris-soybean), and T0 (Fresh semen-tris) was used as control before sexing. The parameters observed were the quality of fresh semen and after sexing. The results of the study showed that the characteristic of Bali bull fresh semen macroscopically in this study was 4.75 mL in volume, cream color, distinctive odor, pH 6.6, moderate consistency, and microscopically motility, viability, abnormality, and concentration were 94.22%, 96.06% 4.89, and 1596×106, respectively. Motility and viability after sexing were significantly (P<0.05) decrease in comparison to the fresh semen (T0) at each treatment both top and bottom layers. In conclusion, the smallest decreased of the sperms motility and viability were still greater than 50%. Different extenders as one of the treatments in the present study did not showing different motility and viability of the sperms. This suggests that the use of tris and soybean or their combination can be used as semen extender for Bali bull sexed semen. Keywords: Soy extender, Bali bull, sexing, motility, viability.

Collaboration network of the authors

20 authors · 47 co-authorship links · 8 across different organisations

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  • Hasanuddin University
  • Vietnam National University of Agriculture
  • Politeknik Pertanian Negeri Pangkajene Kepulauan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Zulkharnaim MaupaAffiliation unknown2
Lellah RahimHasanuddin University2
Sudirman BacoHasanuddin University2
Sahiruddin SahiruddinHasanuddin University2
E. DondatuHasanuddin University2
Muhammad YusufHasanuddin University1
Djoni Prawira RahardjaHasanuddin University1
S. LongVietnam National University of Agriculture1
Masturi MasturiHasanuddin University1
Toleng Abd LatiefAffiliation unknown1
Nur SalmahHasanuddin University1
Hasrin HasrinHasanuddin University1
Ummul MasirPoliteknik Pertanian Negeri Pangkajene Kepulauan1
Ambo AkoHasanuddin University1
Putri Lidia DamayantiHasanuddin University1
Dina ArdianaHasanuddin University1
Zulkharnaim ZulkharnaimHasanuddin University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, first harvested 2 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">The Quality of Bali Bull Sexed Sperm Using Soybean Extract Extender</dc:title>
	<dc:creator xml:lang="en">Nur, Nur Eni</dc:creator>
	<dc:creator xml:lang="en">Toleng, Abdul Latief </dc:creator>
	<dc:creator xml:lang="en">Yusuf, Muhammad</dc:creator>
	<dc:description xml:lang="en">The process of sexing spermatozoa requires a medium that is able to protect and provide an optimal environment. One of them is extender media that is used to extend the volume of semen. The extender that is commonly used is commercially but some limitation such as price and availability. Therefore, it is necessary to have an alternative extender such as soybean. The aim of this study was to know the quality of sexed sperms diluted using soybean extender. Semen of Bali bull was collected for five times and then subjected to three different extender treatments after sexing; T1 (Soybean), T2 (Tris), T3 (Tris-soybean), and T0 (Fresh semen-tris) was used as control before sexing. The parameters observed were the quality of fresh semen and after sexing. The results of the study showed that the characteristic of Bali bull fresh semen macroscopically in this study was 4.75 mL in volume, cream color, distinctive odor, pH 6.6, moderate consistency, and microscopically motility, viability, abnormality, and concentration were 94.22%, 96.06% 4.89, and 1596×106, respectively. Motility and viability after sexing were significantly (P&amp;lt;0.05) decrease in comparison to the fresh semen (T0) at each treatment both top and bottom layers. In conclusion, the smallest decreased of the sperms motility and viability were still greater than 50%. Different extenders as one of the treatments in the present study did not showing different motility and viability of the sperms. This suggests that the use of tris and soybean or their combination can be used as semen extender for Bali bull sexed semen.
Keywords: Soy extender, Bali bull, sexing, motility, viability.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2022-07-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/19439</dc:identifier>
	<dc:identifier>10.20956/hajas.v4i1.19439</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 4 No. 1 (2022); 48-57</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/19439/8464</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>