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Effect of Egg Yolk Powder as An Alternative Extender on The Quality of Bali Bull Spermatozoa

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 6 No. 1 (2024) · pp. 60–67

DOI
10.20956/hajas.v6i1.26956
ISSN
2621-9190, 2621-9182
Published
2024-08-24
Publisher
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Semen extender is closely related to the success of artificial insemination to achieve maximum results. Extender aims to increase the volume of semen, provide food substances as a source of energy for spermatozoa provide buffers to maintain pH, osmotic pressure, and electrolyte balance, and prevent the possibility of germ growth. This study aims to determine the effect of extender on the quality of Bali bull spermatozoa through an alternative approach, one of which uses Tris-Egg Yolk powder extender, which is efficient and effective. The study was designed randomized, with three treatments and five replications. The treatment combinations were P0 (Tris-Fresh Egg Yolk 20%), P1 (Tris Oven Egg Yolk 20%), and P2 (Tris-Commercial Egg Yolk 20%). The results of analysis of variance showed that in the parameters of progressive motility, viability, abnormality and intact plasma membrane, treatment of P0 (Tris-Fresh Egg Yolk 20%) tended to be similar as treatment P1 (Tris-Oven Egg Yolk 20%) (P>0.05), but in P2 (Tris-Commercial Egg Yolk 20%) was significantly lower (P<0.05) than P0 and P1. It can be concluded that oven egg yolk powder mixed with tris solution can maintain sperm quality after dilution. Keywords: Oven egg yolk powder, commercial egg yolk powder, buffer solution

Collaboration network of the authors

18 authors · 43 co-authorship links · 5 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Hasanuddin University
  • Politeknik Pertanian Negeri Pangkajene Kepulauan
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Sudirman BacoHasanuddin University3
Zulkharnaim MaupaAffiliation unknown2
Lellah RahimHasanuddin University2
Sahiruddin SahiruddinHasanuddin University2
Abdul Latief TolengHasanuddin University2
Masturi MasturiHasanuddin University1
Toleng Abd LatiefAffiliation unknown1
Nur SalmahHasanuddin University1
Hasrin HasrinHasanuddin University1
Ummul MasirPoliteknik Pertanian Negeri Pangkajene Kepulauan1
Ambo AkoHasanuddin University1
Putri Lidia DamayantiHasanuddin University1
Nur Eni NurHasanuddin University1
E. DondatuHasanuddin University1
Abdul QayyumHasanuddin University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, first harvested 2 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Effect of Egg Yolk Powder as An Alternative Extender on The Quality of Bali Bull Spermatozoa </dc:title>
	<dc:creator xml:lang="en">Dina Ardiana</dc:creator>
	<dc:creator xml:lang="en">Yusuf, Muhammad</dc:creator>
	<dc:creator xml:lang="en">Zulkharnaim, Zulkharnaim</dc:creator>
	<dc:description xml:lang="en">Semen extender is closely related to the success of artificial insemination to achieve maximum results. Extender aims to increase the volume of semen, provide food substances as a source of energy for spermatozoa provide buffers to maintain pH, osmotic pressure, and electrolyte balance, and prevent the possibility of germ growth. This study aims to determine the effect of extender on the quality of Bali bull spermatozoa through an alternative approach, one of which uses Tris-Egg Yolk powder extender, which is efficient and effective. The study was designed randomized, with three treatments and five replications. The treatment combinations were P0 (Tris-Fresh Egg Yolk 20%), P1 (Tris Oven Egg Yolk 20%), and P2 (Tris-Commercial Egg Yolk 20%). The results of analysis of variance showed that in the parameters of progressive motility, viability, abnormality and intact plasma membrane, treatment of P0 (Tris-Fresh Egg Yolk 20%) tended to be similar as treatment P1 (Tris-Oven Egg Yolk 20%) (P&amp;gt;0.05), but in P2 (Tris-Commercial Egg Yolk 20%) was significantly lower (P&amp;lt;0.05) than P0 and P1. It can be concluded that oven egg yolk powder mixed with tris solution can maintain sperm quality after dilution.
Keywords: Oven egg yolk powder, commercial egg yolk powder, buffer solution</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2024-08-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/26956</dc:identifier>
	<dc:identifier>10.20956/hajas.v6i1.26956</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 6 No. 1 (2024); 60-67</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/26956/11934</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>