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Resistance of Ampicillin, Ceftazidime, and Cefotaxime in Poultry’s Escherichia coli

Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) · Vol. UME No. 1 (2023)

DOI
10.20956/jrvi.v7i1.23766
ISSN
2615-2835, 2614-0187
Published
2023-01-31
Publisher
Hasanuddin University
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

Beta-lactam antibiotics are important antibiotics that are widely used in the field of human and animal health. Ampicillin resistance has been widely reported. Another increase in resistance is 3rd generation cephalosporins. The purpose of this study was to compare the ampicillin resistance profiles in 2019 and 2021 in the same E. coli isolates and to determine the resistance profiles of ampicillin, ceftazidime, and cefotaxime in live chicken E. coli. The research stages were the preparation of isolates; culture on differential selective media and checking the uniformity of bacterial cell morphology; biochemical test; bacterial DNA extraction; uspA gene amplification; visualization of amplification results; manufacture of bacterial suspensions; Kirby-Bauer disk diffusion resistance test; measurement of inhibition zones and determination of isolate status; and compared the ampicillin resistance test data. All isolates were confirmed positive for E. coli. The uspA gene (884 bp) was detected in all isolates. Ampicillin resistance in 2019 and 2021 in the same E. coli isolates when compared, there was no difference. Resistance test showed E. coli was resistant to ampicillin (100%), ceftazidime (15.4%), and cefotaxime (64.5%). The conclusion of the study was that there was no difference between the ampicillin resistance in 2019 and 2021 in E. coli isolates. Escherichia coli in this study had the highest resistance profile to ampicillin, followed by cefotaxime, and the lowest was ceftazidime.

Collaboration network of the authors

40 authors · 171 co-authorship links · showing the 40 most connected

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  • IPB University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Ekowati HandharyaniIPB University2
Evy DamayanthiAffiliation unknown2
Hadi RiyadiIPB University2
Dwi Santy DamayatiAffiliation unknown2
Huda Shalahudin DarusmanAffiliation unknown2
Hera MaheshwariAffiliation unknown1
Etih SudarnikaAffiliation unknown1
Maria Fatima PalupiAffiliation unknown1
Apon Zaenal MustopaAffiliation unknown1
Herman IrawanAffiliation unknown1
Joko PamungkasAffiliation unknown1
Ai HertatiAffiliation unknown1
Rifqiyah Nur UmamiAffiliation unknown1
Nurlaili EkawatiAffiliation unknown1
Sheila ChairunnisaAffiliation unknown1
Jendri MamangkeyAffiliation unknown1
Maritsa NurfatwaAffiliation unknown1
Ela NoviantiAffiliation unknown1
Isti Kartika SariAffiliation unknown1
Dyah Ayu HewajuliAffiliation unknown1
NLP Indi DharmayantiAffiliation unknown1
Safika SafikaAffiliation unknown1
Fitrine EkawastiAffiliation unknown1
Hastuti Handayani S PurbaAffiliation unknown1
Sri Suryatmiati PrihandaniAffiliation unknown1
Susan Maphilindawati NoorAffiliation unknown1
Aswin Rafif KhairullahAffiliation unknown1
Alif Rahman Rohim PuaradaAffiliation unknown1
Sri MurtiniIPB University1
Risa TiuriaAffiliation unknown1
Sayu Putu Yuni ParyatiAffiliation unknown1
Nanis NurhidayahAffiliation unknown1
Ridi ArifAffiliation unknown1
Esdinawan Carakantara SatrijaAffiliation unknown1
Fahri SinulinggaAffiliation unknown1
Ainulyakin ImlaniAffiliation unknown1
Caddoumy Zahrel BenAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/jrvi/oai, first harvested 3 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Resistance of Ampicillin, Ceftazidime, and Cefotaxime in Poultry’s Escherichia coli </dc:title>
	<dc:creator xml:lang="en">Aprilia Hardiati</dc:creator>
	<dc:creator xml:lang="en">Safika</dc:creator>
	<dc:creator xml:lang="en">I Wayan Teguh Wibawan</dc:creator>
	<dc:description xml:lang="en">Beta-lactam antibiotics are important antibiotics that are widely used in the field of human and animal health. Ampicillin resistance has been widely reported. Another increase in resistance is 3rd generation cephalosporins. The purpose of this study was to compare the ampicillin resistance profiles in 2019 and 2021 in the same E. coli isolates and to determine the resistance profiles of ampicillin, ceftazidime, and cefotaxime in live chicken E. coli. The research stages were the preparation of isolates; culture on differential selective media and checking the uniformity of bacterial cell morphology; biochemical test; bacterial DNA extraction; uspA gene amplification; visualization of amplification results; manufacture of bacterial suspensions; Kirby-Bauer disk diffusion resistance test; measurement of inhibition zones and determination of isolate status; and compared the ampicillin resistance test data. All isolates were confirmed positive for E. coli. The uspA gene (884 bp) was detected in all isolates. Ampicillin resistance in 2019 and 2021 in the same E. coli isolates when compared, there was no difference. Resistance test showed E. coli was resistant to ampicillin (100%), ceftazidime (15.4%), and cefotaxime (64.5%). The conclusion of the study was that there was no difference between the ampicillin resistance in 2019 and 2021 in E. coli isolates. Escherichia coli in this study had the highest resistance profile to ampicillin, followed by cefotaxime, and the lowest was ceftazidime.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2023-01-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/jrvi/article/view/23766</dc:identifier>
	<dc:identifier>10.20956/jrvi.v7i1.23766</dc:identifier>
	<dc:source xml:lang="en">Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research); VOLUME 7, No 1, JANUARY 2023</dc:source>
	<dc:source>2615-2835</dc:source>
	<dc:source>2614-0187</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/jrvi/article/view/23766/9251</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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