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Isolation and Identification of Carbonoclastic Fungi Causing Damage to Prehistoric Paintings in the Maros-Pangkep Karst Area

International Journal of Applied Biology · Vol. 8 No. 1 (2024) · pp. 29–40

ISSN
2580-2119, 2580-2410
Published
2024-06-30
Publisher
Hasanuddin University
Language
en

Abstract

The carbonoclastic fungi known as a fungi that can produce calcium carbonate crystals (CaCO3) through the urease enzyme produced to hydrolysis of urea contained in the substrate or growth medium. The presence of carbonoclastic fungi is frequently observed in the karst areas cave wall painting, which are a type of ancient artifact. The destruction to the ancient cave artwork is brought on by the fungus. The purpose of this study was to obtain and determine the types of carbonoclastic fungi that cause damage to cave wall paintings in the Maros-Pangkep karst area. Isolation and selection of carbonoclastic fungi were carried out using Christensen Urea Agar medium. The CaCO3 precipitate potential test was carried out by calculating the mass CaCO3 precipitates formed and analysis of ammonia levels and cell biomass produced during the growth period. Fungi identification was performed using the 18S rRNA gene molecular markers. Twenty four fungi isolates obtained from swab samples in Parewe and Bulu Sipong caves, 7 positive isolated belonged to carbonoclastic fungi. The results of CaCO3 precipitates, namely isolate Ps3 producing precipitates of 80,30 mg with ammonia content value of 701,7064 ppm and cell biomass of 333,80 mg. The identification results showed that Ps3 isolate belongs to Aspergillus sp. strain BW1.

Keywords

Collaboration network of the authors

40 authors · 137 co-authorship links · showing the 40 most connected

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  • Sunan Kalijaga State Islamic University Yogyakarta
  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Fahruddin FahruddinSunan Kalijaga State Islamic University Yogyakarta5
Magdalena LitaayAffiliation unknown3
Nur Umriani PermatasariAffiliation unknown3
Zaraswati DwyanaAffiliation unknown3
Elis TambaruAffiliation unknown2
Ambeng - AmbengAffiliation unknown2
Zaraswati DwayanaAffiliation unknown2
Eva JohanesAffiliation unknown2
Ruslan UmarAffiliation unknown2
Syafrian Nur MuhammadAffiliation unknown2
Nur Afifah ZhafirahAffiliation unknown2
Abdul WahabAffiliation unknown2
Rifaat RifaatAffiliation unknown2
Abdul SalamInstitut Agama Islam Negeri Kendari2
Nur Umriani P UmarAffiliation unknown2
Paulina TabaAffiliation unknown1
Nur AfniAffiliation unknown1
Djabal Nur BasirAffiliation unknown1
Bulkis MusaAffiliation unknown1
Prayiska Mariana RapiAffiliation unknown1
Emmi AstutiAffiliation unknown1
Novi LestariAffiliation unknown1
Nurmala SariAffiliation unknown1
Heriadi HeriadiAffiliation unknown1
Syahribulan SyahribulanAffiliation unknown1
Dody PriosambodoAffiliation unknown1
Erviana TahirAffiliation unknown1
Risco G BudjiAffiliation unknown1
Karlina SariAffiliation unknown1
Risco B GobelAffiliation unknown1
Metty AgustineAffiliation unknown1
Nurul HidayahAffiliation unknown1
Andriany AndrianyAffiliation unknown1
Eva JohannesAffiliation unknown1
Henra -Affiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/ijoab/oai, first harvested 3 Nov 2026, last seen 3 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Isolation and Identification of Carbonoclastic Fungi Causing Damage to Prehistoric Paintings in the Maros-Pangkep Karst Area</dc:title>
	<dc:creator xml:lang="en">Khotimah, Nur Husnul </dc:creator>
	<dc:creator xml:lang="en">Haedar, Nur</dc:creator>
	<dc:creator xml:lang="en">Abdullah, As'adi</dc:creator>
	<dc:creator xml:lang="en">Gani, Fuad </dc:creator>
	<dc:creator xml:lang="en">Heriadi</dc:creator>
	<dc:subject xml:lang="en">Calcium Carbonate; Cave; Fungi; Precipitate.</dc:subject>
	<dc:description xml:lang="en">The carbonoclastic fungi known as a fungi that can produce calcium carbonate crystals (CaCO3) through the urease enzyme produced to hydrolysis of urea contained in the substrate or growth medium. The presence of carbonoclastic fungi is frequently observed in the karst areas cave wall painting, which are a type of ancient artifact. The destruction to the ancient cave artwork is brought on by the fungus. The purpose of this study was to obtain and determine the types of carbonoclastic fungi that cause damage to cave wall paintings in the Maros-Pangkep karst area. Isolation and selection of carbonoclastic fungi were carried out using Christensen Urea Agar medium. The CaCO3 precipitate potential test was carried out by calculating the mass CaCO3 precipitates formed and analysis of ammonia levels and cell biomass produced during the growth period. Fungi identification was performed using the 18S rRNA gene molecular markers. Twenty four fungi isolates obtained from swab samples in Parewe and Bulu Sipong caves, 7 positive isolated belonged to carbonoclastic fungi. The results of CaCO3 precipitates, namely isolate Ps3 producing precipitates of 80,30 mg with ammonia content value of 701,7064 ppm and cell biomass of 333,80 mg. The identification results showed that Ps3 isolate belongs to Aspergillus sp. strain BW1.</dc:description>
	<dc:publisher xml:lang="en">Hasanuddin University</dc:publisher>
	<dc:date>2024-06-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/ijoab/article/view/31772</dc:identifier>
	<dc:source xml:lang="en">International Journal of Applied Biology; Vol. 8 No. 1 (2024): International Journal of Applied Biology; 29-40</dc:source>
	<dc:source>2580-2119</dc:source>
	<dc:source>2580-2410</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/ijoab/article/view/31772/11686</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 International Journal of Applied Biology</dc:rights>
</oai_dc:dc>