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DETECTION OF SHIGELLA SP ON STOOL CHILDREN WITH DIARRHEA USING CULTURE METHODE AND PCR

Nusantara Medical Science Journal · Vol. ume No. 4 (2017) · pp. 133–138

DOI
10.20956/nmsj.v1i4.2250
ISSN
2597-7288, 2460-9757
Published
2017-08-09
Publisher
Faculty of Medicine, Hasanuddin University.
Language
en

Abstract

ABSTRACT Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode. Keywords: Diarrhea, Shigellasp, Culture, PCR

Collaboration network of the authors

14 authors · 24 co-authorship links

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  • Hasanuddin University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Lokita PurnamasariHasanuddin University2
Sartini SartiniAffiliation unknown1
Yenti PurnamasariAffiliation unknown1
Faisal AttamimiAffiliation unknown1
M Natsir DjideAffiliation unknown1
Muhammad Subhan A. SibaduAffiliation unknown1
Nurul Muhlisa MusAffiliation unknown1
Mustika SariHasanuddin University1
Nikmatia LatiefAffiliation unknown1
Rizalinda SjahrilHasanuddin University1
Andi Nur AsrinawatyHasanuddin University1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/jmednus/oai, first harvested 5 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">DETECTION OF SHIGELLA SP ON STOOL CHILDREN WITH DIARRHEA USING CULTURE METHODE AND PCR</dc:title>
	<dc:creator xml:lang="en">Rusdianto, Rusdianto</dc:creator>
	<dc:creator xml:lang="en">Massi, Muh Nasrum</dc:creator>
	<dc:creator xml:lang="en">Ahmad, Ahyar</dc:creator>
	<dc:description xml:lang="en">ABSTRACT Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode. Keywords: Diarrhea, Shigellasp, Culture, PCR</dc:description>
	<dc:publisher xml:lang="en">Faculty of Medicine, Hasanuddin University.</dc:publisher>
	<dc:date>2017-08-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://journal.unhas.ac.id/index.php/jmednus/article/view/2250</dc:identifier>
	<dc:identifier>10.20956/nmsj.v1i4.2250</dc:identifier>
	<dc:source xml:lang="en">Nusantara Medical Science Journal; Volume 1 No. 4 Oktober - Desember 2016; 133-138</dc:source>
	<dc:source>2597-7288</dc:source>
	<dc:source>2460-9757</dc:source>
	<dc:source>10.20956/nmsj.v1i4</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/jmednus/article/view/2250/1243</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 Nusantara Medical Science Journal</dc:rights>
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