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Validation Study of the Method of Analysis of Fe (II) Levels in Blood Addition Tablet Preparations by Wet Destruction and Dry Destruction Methods by UV-Vis Spectrophotometry

Journal of Science and Technology Research for Pharmacy · Vol. 4 No. 2 (2025) · pp. 46–53

DOI
10.15294/jstrp.v4i2.10006
ISSN
2776-0685
Published
2025-07-10
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Iron is one of the micro minerals needed in the human body. One effort to meet iron needs can be done by consuming blood supplement tablets that contain iron. The iron content contained in blood supplement tablets needs to be known by carrying out quality testing to ensure that the preparation contains the specified ingredients and quantities. The UV-Vis spectrophotometer is an alternative method for determining iron levels because it has lower operating costs and is simpler but has high sensitivity. Determining the iron content to be analyzed requires a complexing agent to form colored compounds. The complexing compound often used in this research is phenantorline. The requirement for metal analysis using a UV-Vis Spectrophotometer is that the sample must be in solution form so a digestion process needs to be carried out. Destruction is divided into two, namely dry and wet destruction. This study aims to compare two digestion methods that are commonly used, namely wet digestion and dry digestion in determining Fe (II) levels in blood supplement tablets. In this research, method validation will also be carried out which aims to show that the testing procedures used obtain the expected results consistently and continuously. Validation of analytical methods carried out includes linearity test parameters, detection limit tests, quantitation limit tests, accuracy tests and precision tests.. Linearity is indicated by R2 of 0.9986; detection limit of 1.10 mg/L; quantitation limit of 3.62 mg/L; accuracy of dry digestion and wet digestion between 80-120%; and precision of dry digestion and wet digestion as indicated by an RSD percentage below 2%. Quantitative test results showed that the percentage levels of blood added tablet samples from wet digestion and dry digestion were 91.52% respectively; 100.09%; 96.15%; 100.96%; 100.67%. and 93.39%; 108.29%; 99.47%; 93.47%; and 92.57%.

Keywords

Collaboration network of the authors

15 authors · 20 co-authorship links

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  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Zalza Zalzabila Adelya PutriAffiliation unknown1
AmeliyanaAffiliation unknown1
Prisma Detty NuryanaAffiliation unknown1
Erika WuryaningsihAffiliation unknown1
Muhammad Shidqi Naufal AthallaAffiliation unknown1
Ernessa Indah Rotua SIRAITAffiliation unknown1
Rahma Annisa IzzaniaAffiliation unknown1
Sigit PriatmokoState University of Semarang1
Senda Kartika RakainsaAffiliation unknown1
Dhatu Sekar LarasatiAffiliation unknown1
Neli Syahida Ni’maAffiliation unknown1
Sitarina WidyariniAffiliation unknown1
Nanang FakhrudinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/JSTRP/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Validation Study of the Method of Analysis of Fe (II) Levels in Blood Addition Tablet Preparations by Wet Destruction and Dry Destruction Methods by UV-Vis Spectrophotometry</dc:title>
	<dc:creator xml:lang="en">Nurul Fajar Agustina</dc:creator>
	<dc:creator xml:lang="en">Endah Widhihastuti</dc:creator>
	<dc:subject xml:lang="en">Dry Destruction</dc:subject>
	<dc:subject xml:lang="en">UV-Vis Spectrophotometer of Blood Addition Tablets</dc:subject>
	<dc:subject xml:lang="en">Validation of Analysis Method</dc:subject>
	<dc:subject xml:lang="en">Wet Destruction</dc:subject>
	<dc:description xml:lang="en">Iron is one of the micro minerals needed in the human body. One effort to meet iron needs can be done by consuming blood supplement tablets that contain iron. The iron content contained in blood supplement tablets needs to be known by carrying out quality testing to ensure that the preparation contains the specified ingredients and quantities. The UV-Vis spectrophotometer is an alternative method for determining iron levels because it has lower operating costs and is simpler but has high sensitivity. Determining the iron content to be analyzed requires a complexing agent to form colored compounds. The complexing compound often used in this research is phenantorline. The requirement for metal analysis using a UV-Vis Spectrophotometer is that the sample must be in solution form so a digestion process needs to be carried out. Destruction is divided into two, namely dry and wet destruction. This study aims to compare two digestion methods that are commonly used, namely wet digestion and dry digestion in determining Fe (II) levels in blood supplement tablets. In this research, method validation will also be carried out which aims to show that the testing procedures used obtain the expected results consistently and continuously. Validation of analytical methods carried out includes linearity test parameters, detection limit tests, quantitation limit tests, accuracy tests and precision tests. . Linearity is indicated by R2 of 0.9986; detection limit of 1.10 mg/L; quantitation limit of 3.62 mg/L; accuracy of dry digestion and wet digestion between 80-120%; and precision of dry digestion and wet digestion as indicated by an RSD percentage below 2%. Quantitative test results showed that the percentage levels of blood added tablet samples from wet digestion and dry digestion were 91.52% respectively; 100.09%; 96.15%; 100.96%; 100.67%. and 93.39%; 108.29%; 99.47%; 93.47%; and 92.57%.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-07-10</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/JSTRP/article/view/10006</dc:identifier>
	<dc:identifier>10.15294/jstrp.v4i2.10006</dc:identifier>
	<dc:source xml:lang="en">Journal of Science and Technology Research for Pharmacy; Vol. 4 No. 2 (2024): Journal of Science and Technology Research for Pharmacy; 46-53</dc:source>
	<dc:source>2776-0685</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/JSTRP/article/view/10006/4547</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Journal of Science and Technology Research for Pharmacy</dc:rights>
</oai_dc:dc>