Melola Discovery

← Melola Discovery

Journal

Analisis Kadar Nitric Oxide dan Aktivitas Glutation Peroksidase dalam Darah Operator SPBU di Semarang

Life Science · Vol. 7 No. 1 (2018) · pp. 24–30

ISSN
2528-5009, 2252-6277
Published
2018-04-02
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Semarang merupakan kota dengan penggunaan kendaraan bermotor sebagai alat transportasi yang selalu mengalami peningkatan pada setiap tahunnya. Timbal (Pb) merupakan salah satu logam berat dalam gas buang hasil pembakaran kendaraan bermotor. Timbal yang masuk dalam tubuh menyebabkan peningkatan produksi nitric oxide (NO) sehingga terjadi inaktivasi enzim glutation peroksidase yang merupakan antioksidan endogen akibat peningkatan tersebut. Sampel dalam penelitian ini adalah darah dari 24 operator SPBU di Semarang. Tujuan penelitian ini adalah menganalisis kadar nitric oxide dan aktivitas glutation peroksidase dalam darah operator SPBU di Semarang. Kadar NO diukur menggunakan metode ELISA sedangkan aktivitas glutation peroksidase diukur menggunakan metode spektofotometri. Data diuji normalitas dengan uji Shapiro-Wilk. Rerata kadar NO yang diperoleh sebesar 2,3962 mmol/l dan aktivitas glutation peroksidase sebesar 74,2096 U/g. Hubungan kadar NO dan aktivitas glutation peroksidase diuji menggunakan uji korelasi r-Spearman dengan hasil koefisien korelasi (r) sebesar 0,797 sehingga dapat disimpulkan bahwa terdapat hubungan antara kadar nitric oxide dengan aktivitas glutation peroksidase darah operator SPBU di Semarang. Semarang is a city that using vehicle as main transportation always increase every year. Lead (Pb) is one of the heavy metals in the flue gases of combustion vehicles. Lead enters the body cause increase the production of nitric oxide (NO) resulting in the inactivation enzyme glutathione peroxidase as endogenous antioxidants due to the increase. The sample in this study is the blood of 24 operator stations in Semarang. The purpose of this study was to analyze the levels of nitric oxide and glutathione peroxidase activity in the blood gas station operator in Semarang. Level of NO is measured using ELISA method while glutathione peroxidase activity is measured using a spectrophotometri method. Data are tested for normality by the Shapiro-Wilk test. The average of level NO and glutathione peroxidase wich is got are 2,3962 mmol/l and 74,2096 U/g. The Relation of NO levels and the activity of glutathione peroxidase are tested using r-Spearman correlation test with the correlation coefficient (r) of 0,797. The conclution is a relation between level of NO and glutathione peroxidase in the gas station operator blood in Semarang

Collaboration network of the authors

40 authors · 82 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Nugrahaningsih WHAffiliation unknown5
Wulan ChristijantiAffiliation unknown4
Retno Sri IswariState University of Semarang4
Aditya MariantiAffiliation unknown3
Didik Huswo UtomoAffiliation unknown3
Sri Ratna RahayuAffiliation unknown2
Siti Harnina BintariAffiliation unknown2
Yanuarita TursinawatiAffiliation unknown2
- UmarudinAffiliation unknown2
N. K. DewiAffiliation unknown1
A. M. A. AhmedAffiliation unknown1
Sri Achadi NugraheniAffiliation unknown1
Suryani As'adAffiliation unknown1
Pingkan FristiwiAffiliation unknown1
Fatimah Asri Fadhilah AuliaAffiliation unknown1
Shafa Alya KamilaAffiliation unknown1
widowati saputraAffiliation unknown1
RR. Sri Ratna RahayuAffiliation unknown1
Sumarti SumartiAffiliation unknown1
Salsabila Kinaya Pranindita LalaAffiliation unknown1
Prima Gita IslamiAffiliation unknown1
Dinar Diyah AstutiAffiliation unknown1
Kartika WidiyaningrumAffiliation unknown1
Prima Gita Islami IslamiAffiliation unknown1
Eva Ulfiatus ShofiaAffiliation unknown1
LisdianaAffiliation unknown1
Revinta Nur Aulia RochlyAffiliation unknown1
Anggun Try SetyaningsihAffiliation unknown1
Bagus Hammam FalahAffiliation unknown1
- MustofaAffiliation unknown1
Nana Kariada MartutiAffiliation unknown1
Retno PurwantiAffiliation unknown1
Wiwi IsnaeniState University of Semarang1
Rheza Alfy YuliantoAffiliation unknown1
Priyantini WidiyaningrumAffiliation unknown1
Yuliana AstrianaAffiliation unknown1
Muhammad Rofiq NezarAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Analisis Kadar Nitric Oxide dan Aktivitas Glutation Peroksidase dalam Darah Operator SPBU di Semarang</dc:title>
	<dc:creator>Sa’adah, Siti Rofiatus</dc:creator>
	<dc:creator>Yuniastuti, Ari</dc:creator>
	<dc:creator>Susanti, R.</dc:creator>
	<dc:description xml:lang="en-US">Semarang merupakan kota dengan penggunaan kendaraan bermotor sebagai alat transportasi yang selalu mengalami peningkatan pada setiap tahunnya. Timbal (Pb) merupakan salah satu logam berat dalam gas buang hasil pembakaran kendaraan bermotor. Timbal yang masuk dalam tubuh menyebabkan peningkatan produksi nitric oxide (NO) sehingga terjadi inaktivasi enzim glutation peroksidase yang merupakan antioksidan endogen akibat peningkatan tersebut. Sampel dalam penelitian ini adalah darah dari 24 operator SPBU di Semarang. Tujuan penelitian ini adalah menganalisis kadar nitric oxide dan aktivitas glutation peroksidase dalam darah operator SPBU di Semarang. Kadar NO diukur menggunakan metode ELISA sedangkan aktivitas glutation peroksidase diukur menggunakan metode spektofotometri. Data diuji normalitas dengan uji Shapiro-Wilk. Rerata kadar NO yang diperoleh sebesar 2,3962 mmol/l dan aktivitas glutation peroksidase sebesar 74,2096 U/g. Hubungan kadar NO dan aktivitas glutation peroksidase diuji menggunakan uji korelasi r-Spearman dengan hasil koefisien korelasi (r) sebesar 0,797 sehingga dapat disimpulkan bahwa terdapat hubungan antara kadar nitric oxide dengan aktivitas glutation peroksidase darah operator SPBU di Semarang.
&amp;nbsp;
Semarang is a city that using vehicle as main transportation always increase every year. Lead (Pb) is one of the heavy metals in the flue gases of combustion vehicles. Lead enters the body cause increase the production of nitric oxide (NO) resulting in the inactivation enzyme glutathione peroxidase as endogenous antioxidants due to the increase. The sample in this study is the blood of 24 operator stations in Semarang. The purpose of this study was to analyze the levels of nitric oxide and glutathione peroxidase activity in the blood gas station operator in Semarang. Level of NO is measured using ELISA method while glutathione peroxidase activity is measured using a spectrophotometri method. Data are tested for normality by the Shapiro-Wilk test. The average of level NO and glutathione peroxidase wich is got are 2,3962 mmol/l and 74,2096 U/g. The Relation of NO levels and the activity of glutathione peroxidase are tested using r-Spearman correlation test with the correlation coefficient (r) of 0,797. The conclution is a relation between level of NO and glutathione peroxidase in the gas station operator blood in Semarang</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2018-04-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/25358</dc:identifier>
	<dc:source xml:lang="en-US">Life Science; Vol 7 No 1 (2018): April 2018; 24-30</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/25358/11377</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2018 Life Science</dc:rights>
</oai_dc:dc>