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Peranan Jenis Media, Sumber Hormon Alami dan Teknik Induksi Akar Planlet dalam Aklimatisasi Pule Pandak

Life Science · Vol. 10 No. 2 (2021) · pp. 140–149

DOI
10.15294/lifesci.v10i2.54445
ISSN
2528-5009, 2252-6277
Published
2021-11-30
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Pule pandak is a plant that has medicinal. Pule pandak seeds have a hard coat that makes it difficult to germinate naturally. Plant tissue culture can be an alternative that is able to overcome the difficulty of seed germination which then needs to be acclimatized. Factors that influence the success of acclimatization include media type, induction technique and growth regulators. This study aims to analyze the role of media types, natural hormone sources and plantlet root induction techniques. This study used a randomized block design with three factors, the type of media (cocopeat, vermiculite and sand), natural hormone sources (coconut water and bean sprouts extract) and root induction techniques (in vitro + ex vitro and ex vitro). The parameters observed were the number of roots, number of leaves, plant height and percentage of life. Data were analyzed by three-way Anava and Duncan's Multiple Range Test. The results showed that the type of media had no effect on the number of roots, number of leaves, plant height and percentage of life. Natural hormone sources of bean sprouts extract affect the number of roots, number of leaves and plant height. Root induction techniques in vitro + ex vitro affected number of roots. Pule pandak adalah tanaman yang memiliki khasiat sebagai bahan obat. Biji pule pandak memiliki kulit biji yang keras sehingga sulit berkecambah secara alami. Kultur jaringan tanaman dapat menjadi salah satu alternatif yang mampu mengatasi kesulitan perkecambahan biji yang selanjutnya perlu dilakukan aklimatisasi. Faktor-faktor yang mempengaruhi keberhasilan aklimatisasi antara lain jenis media, teknik induksi dan zat pengatur tumbuh. Penelitian ini bertujuan untuk menganalisis peranan jenis media, sumber hormon alami dan teknik induksi akar planlet. Penelitian ini menggunakan rancangan acak kelompok tiga faktor yaitu jenis media (cocopeat, vermikulit dan pasir), sumber hormon alami (air kelapa dan ekstrak tauge) dan teknik induksi akar (in vitro+ex vitro dan ex vitro). Parameter yang diamati adalah jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Data dianalisis dengan Anava tiga arah dan Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa jenis media tidak berpengaruh terhadap jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Sumber hormon alami ekstrak tauge berpengaruh terhadap jumlah akar, jumlah daun dan tinggi tanaman. Teknik induksi akar secara in vitro+ex vitro berpengaruh terhadap jumlah akar.

Collaboration network of the authors

35 authors · 76 co-authorship links · 6 across different organisations

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Muria Kudus University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Noor Aini HabibahAffiliation unknown3
Yustinus Ulung AnggraitoAffiliation unknown2
Furzani Pa’eeAffiliation unknown2
Siti MasfuahMuria Kudus University1
Ani RusilowatiState University of Semarang1
Fina FakhriyahMuria Kudus University1
Mila RoysaMuria Kudus University1
Wiyanto WiyantoState University of Semarang1
Parmin ParminState University of Semarang1
E. CahyonoState University of Semarang1
Khusniyyatul MunaAffiliation unknown1
Krispinus Kedati PukanState University of Semarang1
Laila Nur Hidayah HidayahAffiliation unknown1
Surya DiantinaAffiliation unknown1
Nugroho Edi KartijonoState University of Semarang1
Erlangga Dwi AndikaAffiliation unknown1
Yermia Dita Kris DAffiliation unknown1
Wahyu Nilam CahyatiAffiliation unknown1
Koriatun WakidahAffiliation unknown1
Mega Rifqi Ainun NajibAffiliation unknown1
Adninta Husnu AmaliaAffiliation unknown1
Ely RudyatmiAffiliation unknown1
Talitha WidiatningrumAffiliation unknown1
Reny RahayuAffiliation unknown1
Muhammad Abdul JabarAffiliation unknown1
Sujatha RamasamyAffiliation unknown1
Nurul Huda Am Zen AriefAffiliation unknown1
Dinda MelatiAffiliation unknown1
Na Dame Maulibulung SinagaAffiliation unknown1
Moh. Solehatul MustofaAffiliation unknown1
Amin RetnoningsihAffiliation unknown1
Bintang Faisal AkbarAffiliation unknown1
Danissa Wirna KarmestiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc)
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	<dc:title xml:lang="en-US">Peranan Jenis Media, Sumber Hormon Alami dan Teknik Induksi Akar Planlet dalam Aklimatisasi Pule Pandak</dc:title>
	<dc:creator>Rochmah, Siti</dc:creator>
	<dc:creator>Rahayu, Enni Suwarsi</dc:creator>
	<dc:description xml:lang="en-US">Pule pandak is a plant that has medicinal. Pule pandak seeds have a hard coat that makes it difficult to germinate naturally. Plant tissue culture can be an alternative that is able to overcome the difficulty of seed germination which then needs to be acclimatized. Factors that influence the success of acclimatization include media type, induction technique and growth regulators. This study aims to analyze the role of media types, natural hormone sources and plantlet root induction techniques. This study used a randomized block design with three factors, the type of media (cocopeat, vermiculite and sand), natural hormone sources (coconut water and bean sprouts extract) and root induction techniques (in vitro + ex vitro and ex vitro). The parameters observed were the number of roots, number of leaves, plant height and percentage of life. Data were analyzed by three-way Anava and Duncan's Multiple Range Test. The results showed that the type of media had no effect on the number of roots, number of leaves, plant height and percentage of life. Natural hormone sources of bean sprouts extract affect the number of roots, number of leaves and plant height. Root induction techniques in vitro + ex vitro affected number of roots.
&amp;nbsp;
Pule pandak adalah tanaman yang memiliki khasiat sebagai bahan obat. Biji pule pandak memiliki kulit biji yang keras sehingga sulit berkecambah secara alami. Kultur jaringan tanaman dapat menjadi salah satu alternatif yang mampu mengatasi kesulitan perkecambahan biji yang selanjutnya perlu dilakukan aklimatisasi. Faktor-faktor yang mempengaruhi keberhasilan aklimatisasi antara lain jenis media, teknik induksi dan zat pengatur tumbuh. Penelitian ini bertujuan untuk menganalisis peranan jenis media, sumber hormon alami dan teknik induksi akar planlet. Penelitian ini menggunakan rancangan acak kelompok tiga faktor yaitu jenis media (cocopeat, vermikulit dan pasir), sumber hormon alami (air kelapa dan ekstrak tauge) dan teknik induksi akar (in vitro+ex vitro dan ex vitro). Parameter yang diamati adalah jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Data dianalisis dengan Anava tiga arah dan Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa jenis media tidak berpengaruh terhadap jumlah akar, jumlah daun, tinggi tanaman dan persentase hidup. Sumber hormon alami ekstrak tauge berpengaruh terhadap jumlah akar, jumlah daun dan tinggi tanaman. Teknik induksi akar secara in vitro+ex vitro berpengaruh terhadap jumlah akar.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2021-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:identifier>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/54445</dc:identifier>
	<dc:identifier>10.15294/lifesci.v10i2.54445</dc:identifier>
	<dc:source xml:lang="en-US">Life Science; Vol 10 No 2 (2021): November 2021; 140-149</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:source>10.15294/lifesci.v10i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/54445/20998</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2021 Life Science</dc:rights>
	<dc:rights xml:lang="en-US">https://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci</dc:rights>
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