Journal
Selection of Potential Lignin-Degrading Bacteria and Fungi Isolates from the Gunung Lumut and Sungai Wain in East Kalimantan
Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · pp. 156–168
- ISSN
- 2338-7610, 2085-191X
- Published
- 2025-04-20
- Publisher
- Universitas Negeri Semarang
- Language
- eng
Abstract
The enzymes laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP) are ligninolytic enzyme groups that have a crucial role in degrading lignin compounds. These complex components are abundant in plant biomass. This research aims to isolate and characterize microorganisms producing the enzymes Lac, MnP, and LiP from leaf litter, rhizosphere soil, and sediment in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan. The bacterial and fungal isolates obtained were cultured on NA and PDA media enriched with 0.05% guaiacol as a substrate. Initial selection was carried out based on the colony growth rate and the formation of a brownish discoloration zone, which indicates ligninolytic enzyme activity. The superior isolates were then tested for extracellular enzyme activity quantitatively using a UV-Vis spectrophotometer. Among the bacterial isolates, the highest Lac activity is isolated GLS 5.1.2 (173.44 U L-1); the highest MnP activity is isolated GLS 5.3 (177.41 U L-1); and the highest LiP activity is isolated GLS 5.1.2 (1034.05 U L-1). In the group of fungi, isolate SS2 was detected with the highest Lac activity (20.66 U L-1), MnP activity (333.75 U L-1), and LiP activity (2516.13 U L-1). Biodiversity sources in the Gunung Lumut Protected Forest (GLPF) and Sungai Wain Protected Forest (SWPF), East Kalimantan have the potential to produce microbial isolates with unique enzymatic characteristics that have never been reported before. The resulting microbial isolate can be used as a bioactivator in composting organic waste to produce high-quality organic fertilizer.
Keywords
Collaboration network of the authors
35 authors · 98 co-authorship links
Hover or tap a name to highlight its co-authors. Double-click to open the author's page.
- Cenderawasih University
- Affiliation unknown
- Different organisations
- Same organisation
- Affiliation unknown
- Thicker line = more joint articles · bigger circle = more articles
Show as table
| Co-author | Organisation | Joint articles |
|---|---|---|
| Abdul Hafid | Affiliation unknown | 2 |
| EDOWARD K RAUNSAY | Affiliation unknown | 1 |
| Matilda Y. Wambrauw | Affiliation unknown | 1 |
| Ruth Megawati | Cenderawasih University | 1 |
| Yayan Sopian | Affiliation unknown | 1 |
| Nelince Fakdawer | Affiliation unknown | 1 |
| Merry M. Bonai | Affiliation unknown | 1 |
| Ferdiansyah Ritonga | Affiliation unknown | 1 |
| Sahajuddin | Affiliation unknown | 1 |
| Ansaar | Affiliation unknown | 1 |
| Erlita Tantri | Affiliation unknown | 1 |
| Sri Najiyati | Affiliation unknown | 1 |
| Sahajuddin Sahajuddin | Affiliation unknown | 1 |
| Reza Amarta Prayoga | Affiliation unknown | 1 |
| Ansaar Ansaar | Affiliation unknown | 1 |
| Sritimuryati Sritimuryati | Affiliation unknown | 1 |
| Giyanto Giyanto | Affiliation unknown | 1 |
| Novik Nurhidayat | Affiliation unknown | 1 |
| Dori Kusuma Jaya | Affiliation unknown | 1 |
| Dea Indriani Astuti | Affiliation unknown | 1 |
| Achmad Dinoto | Affiliation unknown | 1 |
| Oscar Efendy | Affiliation unknown | 1 |
| Heddy Julistiono | Affiliation unknown | 1 |
| Suharno Suharno | Cenderawasih University | 1 |
| Irma Rahayu | Cenderawasih University | 1 |
| Rosye H.R. Tanjung | Affiliation unknown | 1 |
Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.