First Record of Free-Living Nematode Ironus dentifurcatus Argo & Heyns, 1972 (Ironidae: Enoplida) From Indonesia

Pradana, Ankardiansyah Pandu; Yoshiga, Toyoshi

doi:10.15294/biosaintifika.v17i1.20990

Journal

First Record of Free-Living Nematode Ironus dentifurcatus Argo & Heyns, 1972 (Ironidae: Enoplida) From Indonesia

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · pp. 41–49

DOI: 10.15294/biosaintifika.v17i1.20990
ISSN: 2338-7610, 2085-191X
Published: 2025-04-20
Publisher: Universitas Negeri Semarang
Language: eng

Abstract

Free-living nematodes often serve as the bioindicators of environmental quality. These nematodes are highly sensitive to even minute changes in the environment. Studies related to the presence of free-living nematodes have flourished, but to date, only a few studies have investigated the morphological and molecular characterization of free-living nematodes from agroecosystems in Indonesia. This study aimed to characterize the free-living nematode Ironus spp. from the rhizosphere of Arabica coffee plants in Bondowoso Regency, Indonesia. Nematodes were extracted from the soil using the white-head tray method and then preserved in a DESS solution to maintain their morphology and DNA. The morphometric analysis of Ironus species obtained in this study showed that the nematode species is identical to Ironus dentifurcatus. The small subunit (SSU) region of rDNA of the nematode was successfully amplified using primers (SSU F07 and SSU R 81). The results of phylogenetic analysis and sequence homology showed that the sequence in this study showed up to 95% homology to Ironus spp. In the database. This is the first study documenting I. dentifurcatus in Indonesia that integrates morphological and molecular characterization.

Keywords

Collaboration network of the authors

10 authors · 37 co-authorship links

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

Affiliation unknown

Different organisations
Same organisation
Affiliation unknown
Thicker line = more joint articles · bigger circle = more articles

Show as table

Co-author	Organisation	Joint articles
Mohammad Hoesain	Affiliation unknown	1
Rachmi Masnilah	Affiliation unknown	1
Fariz Kustiawan Alfarisy	Affiliation unknown	1
Suhartiningsih Dwi Nurchayanti	Affiliation unknown	1
Dwi Tirta Sari	Affiliation unknown	1
Farchan Mushal Al Ramadhan	Affiliation unknown	1
Rismayani	Affiliation unknown	1
Sri Ita Tarigan	Affiliation unknown	1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.

Original metadata (oai_dc)

<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">First Record of Free-Living Nematode Ironus dentifurcatus Argo &amp; Heyns, 1972 (Ironidae: Enoplida) From Indonesia</dc:title>
	<dc:creator xml:lang="en">Pradana, Ankardiansyah Pandu</dc:creator>
	<dc:creator xml:lang="en">Yoshiga, Toyoshi</dc:creator>
	<dc:subject xml:lang="en">18S rDNA </dc:subject>
	<dc:subject xml:lang="en">agroecosystem</dc:subject>
	<dc:subject xml:lang="en">rhizosphere</dc:subject>
	<dc:subject xml:lang="en">morphometry</dc:subject>
	<dc:subject xml:lang="en">SSU (Small Subunit)</dc:subject>
	<dc:description xml:lang="en">Free-living nematodes often serve as the bioindicators of environmental quality. These nematodes are highly sensitive to even minute changes in the environment. Studies related to the presence of free-living nematodes have flourished, but to date, only a few studies have investigated the morphological and molecular characterization of free-living nematodes from agroecosystems in Indonesia. This study aimed to characterize the free-living nematode Ironus spp. from the rhizosphere of Arabica coffee plants in Bondowoso Regency, Indonesia. Nematodes were extracted from the soil using the white-head tray method and then preserved in a DESS solution to maintain their morphology and DNA. The morphometric analysis of Ironus species obtained in this study showed that the nematode species is identical to Ironus dentifurcatus. The small subunit (SSU) region of rDNA of the nematode was successfully amplified using primers (SSU F07 and SSU R 81). The results of phylogenetic analysis and sequence homology showed that the sequence in this study showed up to 95% homology to Ironus spp. In the database. This is the first study documenting I. dentifurcatus in Indonesia that integrates morphological and molecular characterization.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20990</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.20990</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 41-49</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20990/3611</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>