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First Record of Free-Living Nematode Ironus dentifurcatus Argo & Heyns, 1972 (Ironidae: Enoplida) From Indonesia

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · pp. 41–49

DOI
10.15294/biosaintifika.v17i1.20990
ISSN
2338-7610, 2085-191X
Published
2025-04-20
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Free-living nematodes often serve as the bioindicators of environmental quality. These nematodes are highly sensitive to even minute changes in the environment. Studies related to the presence of free-living nematodes have flourished, but to date, only a few studies have investigated the morphological and molecular characterization of free-living nematodes from agroecosystems in Indonesia. This study aimed to characterize the free-living nematode Ironus spp. from the rhizosphere of Arabica coffee plants in Bondowoso Regency, Indonesia. Nematodes were extracted from the soil using the white-head tray method and then preserved in a DESS solution to maintain their morphology and DNA. The morphometric analysis of Ironus species obtained in this study showed that the nematode species is identical to Ironus dentifurcatus. The small subunit (SSU) region of rDNA of the nematode was successfully amplified using primers (SSU F07 and SSU R 81). The results of phylogenetic analysis and sequence homology showed that the sequence in this study showed up to 95% homology to Ironus spp. In the database. This is the first study documenting I. dentifurcatus in Indonesia that integrates morphological and molecular characterization.

Keywords

Collaboration network of the authors

33 authors · 121 co-authorship links · 3 across different organisations

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  • Universitas Jember
  • Universitas Borneo Tarakan
  • Universitas Nurul Jadid
  • Siliwangi University
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Mohammad HoesainAffiliation unknown3
Fariz Kustiawan AlfarisyAffiliation unknown3
Muh. AdiwenaUniversitas Borneo Tarakan3
Sri WahyuningsihAffiliation unknown2
Rachmi MasnilahAffiliation unknown1
Suhartiningsih Dwi NurchayantiAffiliation unknown1
Dwi Tirta SariAffiliation unknown1
Farchan Mushal Al RamadhanAffiliation unknown1
RismayaniAffiliation unknown1
Sri Ita TariganAffiliation unknown1
Diana PutriAffiliation unknown1
Deril Indana DamayantiAffiliation unknown1
Ahmed Ibrahim Alrashid YousifAffiliation unknown1
Sigit PrastowoAffiliation unknown1
Ali WafaAffiliation unknown1
HARDIAN SUSILO ADDYAffiliation unknown1
Widi Kusuma WardhaniAffiliation unknown1
Zul Fauzi Nugroho HadiAffiliation unknown1
Nanang Tri HaryadiAffiliation unknown1
Anita FirnandaAffiliation unknown1
Sofia SofiaUniversitas Nurul Jadid1
Yuli HariyatiUniversitas Jember1
Sudarko SudarkoUniversitas Jember1
Mochammad Wildan JadmikoAffiliation unknown1
Khaerani NurlaelitaAffiliation unknown1
Dyah Retno AnggrainiAffiliation unknown1
Dyah Ayu SavitriAffiliation unknown1
Siswoyo SoekarnoAffiliation unknown1
Farchan Mushaf Al RamadhaniAffiliation unknown1
Dwi ApriyaniSiliwangi University1
M MardhianaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">First Record of Free-Living Nematode Ironus dentifurcatus Argo &amp; Heyns, 1972 (Ironidae: Enoplida) From Indonesia</dc:title>
	<dc:creator xml:lang="en">Pradana, Ankardiansyah Pandu</dc:creator>
	<dc:creator xml:lang="en">Yoshiga, Toyoshi</dc:creator>
	<dc:subject xml:lang="en">18S rDNA </dc:subject>
	<dc:subject xml:lang="en">agroecosystem</dc:subject>
	<dc:subject xml:lang="en">rhizosphere</dc:subject>
	<dc:subject xml:lang="en">morphometry</dc:subject>
	<dc:subject xml:lang="en">SSU (Small Subunit)</dc:subject>
	<dc:description xml:lang="en">Free-living nematodes often serve as the bioindicators of environmental quality. These nematodes are highly sensitive to even minute changes in the environment. Studies related to the presence of free-living nematodes have flourished, but to date, only a few studies have investigated the morphological and molecular characterization of free-living nematodes from agroecosystems in Indonesia. This study aimed to characterize the free-living nematode Ironus spp. from the rhizosphere of Arabica coffee plants in Bondowoso Regency, Indonesia. Nematodes were extracted from the soil using the white-head tray method and then preserved in a DESS solution to maintain their morphology and DNA. The morphometric analysis of Ironus species obtained in this study showed that the nematode species is identical to Ironus dentifurcatus. The small subunit (SSU) region of rDNA of the nematode was successfully amplified using primers (SSU F07 and SSU R 81). The results of phylogenetic analysis and sequence homology showed that the sequence in this study showed up to 95% homology to Ironus spp. In the database. This is the first study documenting I. dentifurcatus in Indonesia that integrates morphological and molecular characterization.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20990</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.20990</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 41-49</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/20990/3611</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
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