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Effects of Autotomy on Haemolymph Profile and Mineral Composition in Mud Crab (Scylla serrata) at Pre and Post- Moulting Cycle

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 3 (2025) · pp. 466–477

DOI
10.15294/biosaintifika.v17i3.24303
ISSN
2338-7610, 2085-191X
Published
2025-11-18
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Soft-shell crab is a valuable product variant in aquaculture, with moulting being a critical phase for its production. Physiological indicators such as haemolymph profiles and mineral content in muscle tissue are essential for assessing crab health and moulting performance. This study aimed to evaluate the effect of autotomy treatment on the physiological condition of mud crab (Scylla serrata), focusing on haemolymph characteristics and mineral concentrations in muscle during the pre-moulting and post-moulting phases. The treatment involved inducing autotomy of walking legs and claws. Sampling was conducted at four distinct phases: pre-autotomy, 15 hours post-autotomy, at immediate post-moult stage, and two days post-moulting. The physiological parameters observed included Total Haemocyte Count (THC) and Differential Haemocyte Count (DHC), comprising hyaline, semi-granular, and granular cell types. Muscle mineral content was assessed by measuring calcium, sodium, and phosphorus concentrations. Results indicated that autotomy reduced THC and the proportion of hyaline cells, while granular cells increased during the post-moulting phase. Calcium, sodium, and phosphorus concentrations decreased shortly after autotomy, peaked during moulting, and declined again after moulting. These findings provide insight into the physiological responses of mud crabs to autotomy and offer practical reference points for enhancing moulting success and supporting soft-shell crab production in aquaculture systems.

Keywords

Collaboration network of the authors

40 authors · 102 co-authorship links · 6 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Bina Sarana Informatika
  • State University of Makassar
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Agus KurniaUniversitas Halu Oleo17
Muhaimin HamzahUniversitas Halu Oleo8
La Ode Baytul AbidinAffiliation unknown5
Oce AstutiAffiliation unknown5
. YusnainiAffiliation unknown4
Wa IbaUniversitas Halu Oleo4
La SaraUniversitas Halu Oleo3
Abdul Muis BalubiAffiliation unknown3
Abdul H. SaritaAffiliation unknown2
Utama Kurnia PangerangUniversitas Halu Oleo2
Muhammad RamliAffiliation unknown2
Asnani AsnaniAffiliation unknown2
Indrayani IndrayaniState University of Makassar2
Utama K. PangerangAffiliation unknown2
Rahmad S. PatadjaiAffiliation unknown2
Muhammad Natsir NessaAffiliation unknown1
Muhammad Iqbal DjawadAffiliation unknown1
Dody Dharmawan TrijunoAffiliation unknown1
Mulyadi MulyadiAffiliation unknown1
Muhammad Irfan BasriUniversitas Halu Oleo1
Abdul HamidUniversitas Bina Sarana Informatika1
Kangkuso AnaluddinUniversitas Halu Oleo1
La SyahrirUniversitas Halu Oleo1
LD Muhammad Yasir HayaAffiliation unknown1
Asnani AsnaniAffiliation unknown1
Khaidhar Ma’rufUniversitas Halu Oleo1
Yustika Intan PermatahatiAffiliation unknown1
Muhammad RamliUniversitas Halu Oleo1
Zainuddin SaenongUniversitas Halu Oleo1
Muhammad IdrisUniversitas Halu Oleo1
Emand Syapriawan TolanamyUniversitas Halu Oleo1
Rahmad Sofyan PatadjaiAffiliation unknown1
Sitti Asna SamuriaUniversitas Halu Oleo1
Muzuni MuzuniAffiliation unknown1
Muh Yamin EffendieAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Effects of Autotomy on Haemolymph Profile and Mineral Composition in Mud Crab (Scylla serrata) at Pre and Post- Moulting Cycle</dc:title>
	<dc:creator xml:lang="en">Yusnaini, Yusnaini</dc:creator>
	<dc:creator xml:lang="en">Yanti, Esri </dc:creator>
	<dc:creator xml:lang="en">Sabilu, Kadir </dc:creator>
	<dc:creator xml:lang="en">Fekri, Latifa </dc:creator>
	<dc:creator xml:lang="en">Nur, Indriyani </dc:creator>
	<dc:subject xml:lang="en">soft-shell crab</dc:subject>
	<dc:subject xml:lang="en">autotomy-induced moulting</dc:subject>
	<dc:subject xml:lang="en">haemocyte profile</dc:subject>
	<dc:subject xml:lang="en">crab physiology</dc:subject>
	<dc:subject xml:lang="en">aquaculture management</dc:subject>
	<dc:description xml:lang="en">Soft-shell crab is a valuable product variant in aquaculture, with moulting being a critical phase for its production. Physiological indicators such as haemolymph profiles and mineral content in muscle tissue are essential for assessing crab health and moulting performance. This study aimed to evaluate the effect of autotomy treatment on the physiological condition of mud crab (Scylla serrata), focusing on haemolymph characteristics and mineral concentrations in muscle during the pre-moulting and post-moulting phases. The treatment involved inducing autotomy of walking legs and claws. Sampling was conducted at four distinct phases: pre-autotomy, 15 hours post-autotomy, at immediate post-moult stage, and two days post-moulting. The physiological parameters observed included Total Haemocyte Count (THC) and Differential Haemocyte Count (DHC), comprising hyaline, semi-granular, and granular cell types. Muscle mineral content was assessed by measuring calcium, sodium, and phosphorus concentrations. Results indicated that autotomy reduced THC and the proportion of hyaline cells, while granular cells increased during the post-moulting phase. Calcium, sodium, and phosphorus concentrations decreased shortly after autotomy, peaked during moulting, and declined again after moulting. These findings provide insight into the physiological responses of mud crabs to autotomy and offer practical reference points for enhancing moulting success and supporting soft-shell crab production in aquaculture systems.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-11-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/24303</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i3.24303</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 3 (2025): November 2025; 466-477</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/24303/7090</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>