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Therapeutic Potential of Ethanol Extract from Durian Peel on Testes Microanatomy in Diabetic Male Rats

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 3 (2025) · pp. 502–513

DOI
10.15294/biosaintifika.v17i3.25448
ISSN
2338-7610, 2085-191X
Published
2025-11-18
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Diabetes mellitus (DM) is a degenerative disease that can lead to complications such as testicular atrophy. As DM requires lifelong treatment, natural and safe therapeutic alternatives are needed. Durian peel, as a natural material, has potential. This study investigated the effects of ethanol extract of durian peel (EEDP) on testicular histology in diabetic rats. A total of 25 male Sprague-Dawley rats, aged 2 months and weight ±200 g, were randomly assigned to five groups: healthy (K0), diabetic (K1), and diabetic+EEDP at doses of 500 (K2), 750 (K3), and 1,000 mg/kgBW (K4), given orally for 28 days. After treatment, the rats were euthanized, and their testes were removed, weighed, and examined using hematoxylin-eosin staining. Observed variables included testis weight, seminiferous tubule diameter, epithelial thickness, and spermatogenesis stages. Statistical analysis ANOVA (α=0,05) showed significant improvements (p < 0,05) in the EEDP-treated groups. It is concluded that EEDP at doses of 500-1,000 mg/kgBW improved testicular structure in diabetic rats.. The novelty of this study lies in the utilization of durian peel ethanol extract at varying doses of 500–1,000 mg/kgBW as a natural therapy to improve testicular histological damage caused by diabetes. This study serves as a basis for the development of herbal antidiabetic drugs with protective effects on the reproductive system. The findings are expected to contribute to biomedical science and provide opportunities for utilizing durian peel waste as a value-added material for public health.

Keywords

Collaboration network of the authors

26 authors · 93 co-authorship links

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  • State University of Semarang
  • Affiliation unknown
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Co-authorOrganisationJoint articles
La Ode Irman JayaAffiliation unknown5
Kustopo BudiraharjoAffiliation unknown3
Zalfa AlfatinnisaAffiliation unknown2
Mohammad Wahyu AndriyanAffiliation unknown2
Muhammad Ragil SaputraAffiliation unknown2
Endah Puji AstutiAffiliation unknown2
Agus SubagioAffiliation unknown2
Dwinanda BudiwardaniAffiliation unknown2
Langlang HandayaniAffiliation unknown1
Teguh DarsonoAffiliation unknown1
Slamet HaryonoAffiliation unknown1
Usrek Tani UtinaAffiliation unknown1
Haydnn Caesha MaulanaAffiliation unknown1
Musa Abdul HalimAffiliation unknown1
Iklas Adi PangestuAffiliation unknown1
Nabila Rakha PutriAffiliation unknown1
Masturi MasturiState University of Semarang1
Yohannes SardjonoAffiliation unknown1
Rio Agustian Gilang FernandoAffiliation unknown1
Pratiwi DwijanantiAffiliation unknown1
Maisya Sifana MauraAffiliation unknown1
Muhammad Anwar DjaelaniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Therapeutic Potential of Ethanol Extract from Durian Peel on Testes Microanatomy in Diabetic Male Rats</dc:title>
	<dc:creator xml:lang="en">Syakera, Cinta Etna </dc:creator>
	<dc:creator xml:lang="en">Isdadiyanto, Sri </dc:creator>
	<dc:creator xml:lang="en">Sunarno , Sunarno </dc:creator>
	<dc:creator xml:lang="en">Ermanjaya, Laode </dc:creator>
	<dc:subject xml:lang="en">ethanol extract</dc:subject>
	<dc:subject xml:lang="en">durian peel</dc:subject>
	<dc:subject xml:lang="en">diabetic</dc:subject>
	<dc:subject xml:lang="en">antihyperglycemic</dc:subject>
	<dc:subject xml:lang="en">testes  </dc:subject>
	<dc:description xml:lang="en">Diabetes mellitus (DM) is a degenerative disease that can lead to complications such as testicular atrophy. As DM requires lifelong treatment, natural and safe therapeutic alternatives are needed. Durian peel, as a natural material, has potential. This study investigated the effects of ethanol extract of durian peel (EEDP) on testicular histology in diabetic rats. A total of 25 male Sprague-Dawley rats, aged 2 months and weight ±200 g, were randomly assigned to five groups: healthy (K0), diabetic (K1), and diabetic+EEDP at doses of 500 (K2), 750 (K3), and 1,000 mg/kgBW (K4), given orally for 28 days. After treatment, the rats were euthanized, and their testes were removed, weighed, and examined using hematoxylin-eosin staining. Observed variables included testis weight, seminiferous tubule diameter, epithelial thickness, and spermatogenesis stages. Statistical analysis ANOVA (α=0,05) showed significant improvements (p &amp;lt; 0,05) in the EEDP-treated groups. It is concluded that EEDP at doses of 500-1,000 mg/kgBW improved testicular structure in diabetic rats.. The novelty of this study lies in the utilization of durian peel ethanol extract at varying doses of 500–1,000 mg/kgBW as a natural therapy to improve testicular histological damage caused by diabetes. This study serves as a basis for the development of herbal antidiabetic drugs with protective effects on the reproductive system. The findings are expected to contribute to biomedical science and provide opportunities for utilizing durian peel waste as a value-added material for public health.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-11-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/25448</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i3.25448</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 3 (2025): November 2025; 502-513</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/25448/7092</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>