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Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 3 (2025) · pp. 382–394

DOI
10.15294/biosaintifika.v17i3.26663
ISSN
2338-7610, 2085-191X
Published
2025-11-18
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.

Keywords

Collaboration network of the authors

40 authors · 137 co-authorship links · 2 across different organisations · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • State University of Semarang
  • Universitas Bhayangkara Surabaya
  • Universitas Negeri Surabaya
  • Cenderawasih University
  • Lambung Mangkurat University
  • Universitas Jember
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Reni AmbarwatiAffiliation unknown4
Haryono HaryonoUniversitas Bhayangkara Surabaya4
Nia KurniawanAffiliation unknown4
Winarsih WinarsihState University of Semarang3
Sunu KuntjoroAffiliation unknown3
Widowati BudijastutiAffiliation unknown3
Diva PitalokaAffiliation unknown2
Isnawati -Universitas Negeri Surabaya1
Guntur TrimulyonoAffiliation unknown1
Mahanani Tri AsriAffiliation unknown1
Fathul HamdaniAffiliation unknown1
Ana FauziaAffiliation unknown1
Muhammad Azimuddin MohamedAffiliation unknown1
SRI WAHYUNIAffiliation unknown1
Fena Dheta SukmaAffiliation unknown1
Isa SuhadaAffiliation unknown1
Soraya FirdausiLambung Mangkurat University1
Supeno SupenoCenderawasih University1
Ulin NuhaAffiliation unknown1
Rizka Elan FadilahAffiliation unknown1
Ferry Budi PrasetyaAffiliation unknown1
Fauziyatul IffahAffiliation unknown1
Achmad Faisal MasruriAffiliation unknown1
Li Yi XuanAffiliation unknown1
Pramudya Dwi Aristya PutraUniversitas Jember1
Ifatur RohmawatiAffiliation unknown1
Maharani Dyah ArumsariAffiliation unknown1
Ahmad Misbakhus SururiAffiliation unknown1
Excelino HendrataAffiliation unknown1
Rahmadian Kahfi Zulfa KamilaAffiliation unknown1
Dhea Mutiara Fernanda WibowoAffiliation unknown1
Luluatul FajriyahAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia</dc:title>
	<dc:creator xml:lang="en">Panjaitan, Viona Angelina Erlan </dc:creator>
	<dc:creator xml:lang="en">Rangga, Adam Dwi </dc:creator>
	<dc:creator xml:lang="en">Sulung, Cahya Ajeng Valenta Tresna </dc:creator>
	<dc:creator xml:lang="en">Rahayu, Dwi Anggorowati</dc:creator>
	<dc:creator xml:lang="en">Khaleyla, Firas </dc:creator>
	<dc:creator xml:lang="en">Nugroho, Endik Deni </dc:creator>
	<dc:creator xml:lang="en">Rusdianto, Rusdianto </dc:creator>
	<dc:creator xml:lang="en">Mamat, Noorhidayah Binti </dc:creator>
	<dc:subject xml:lang="en">diagnostic characters</dc:subject>
	<dc:subject xml:lang="en">DNA barcodes</dc:subject>
	<dc:subject xml:lang="en">Genetic composition</dc:subject>
	<dc:subject xml:lang="en">Phenetic</dc:subject>
	<dc:description xml:lang="en">Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-11-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/26663</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i3.26663</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 3 (2025): November 2025; 382-394</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/26663/7112</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
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