Melola Discovery

← Melola Discovery

Journal

GC-MS Profiles, Anti-inflammatory and Antibacterial Activities of Mucuna pruriens Seed Against Gastrointestinal Pathogens

Biosaintifika: Journal of Biology & Biology Education · Vol. 18 No. 1 (2026) · pp. 78–92

DOI
10.15294/biosaintifika.v18i1.33258
ISSN
2338-7610, 2085-191X
Published
2026-04-28
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

Escherichia coli and Bacillus cereus are pathogenic bacteria commonly implicated in gastrointestinal infection (GI). Severe GI can lead to some gastrointestinal diseases, such as diarrhea, gastroenteritis, and chronic inflammation. While antibiotic therapy remains the standard treatment, the rise in antimicrobial resistance (AMR) cases necessitates alternative therapeutic approaches. This study aimed to investigate the antibacterial and anti-inflammatory potential of Velvet bean (Mucuna pruriens L.) and to identify its bioactive compounds using gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was assessed against E. coli and B. cereus using the disc diffusion method. Meanwhile, anti-inflammatory activity was measured by the red blood cell membrane stabilization method at extract concentrations of 15, 60, 250, and 1000 ppm. Antibacterial effectiveness was determined by measuring the diameter of inhibition zones, while anti-inflammatory potential was quantified through stability percentages. The results showed that the velvet bean’s ethanolic extract had antibacterial activity against both bacteria, with the strongest inhibition observed at 100% extract concentration, producing inhibition zones of 9.2 mm on E. coli and 7.15 mm on B. cereus. For the anti-inflammatory assay, the 1000 ppm extract demonstrated the highest stability value (76.36%), nearly equivalent to the standard non-steroidal anti-inflammatory drug (NSAID), ibuprofen (76.35% at 120 ppm), as a positive control. GC-MS analysis revealed the presence of 18 bioactive compounds in the velvet bean extract, classified into nine chemical groups, which are mostly known for their antimicrobial and anti-inflammatory potential. These findings highlight the potential of M. pruriens to address gastrointestinal infections, particularly those caused by E. coli and B. cereus, as well as its anti-inflammatory effect. This also contributes to achieving sustainable development goals (SDGs), especially to encourage good health in the community through utilizing local resources for herbal-based treatment.

Keywords

Collaboration network of the authors

40 authors · 118 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Padjadjaran University
  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Opik TaupiqurrohmanAffiliation unknown4
Iman HernamanPadjadjaran University3
Tri CahyantoAffiliation unknown2
Ateng SupriyatnaAffiliation unknown2
Astuti KusumoriniAffiliation unknown2
sumiyati Sa’adahAffiliation unknown2
Isma Dwi KurniawanAffiliation unknown2
Cecep HidayatAffiliation unknown1
Opik Taupik KurahmanAffiliation unknown1
Mashuri MasriAffiliation unknown1
Astri YuliawatiAffiliation unknown1
Lusi HaerunnisaAffiliation unknown1
Asep SupriadinAffiliation unknown1
Fauziah Qurota AyuniAffiliation unknown1
Epa PaujiahAffiliation unknown1
Maratus SolikhaAffiliation unknown1
Muhammad YusufUniversitas Halu Oleo1
Toto SubrotoAffiliation unknown1
Sukma NuswantaraAffiliation unknown1
Dina AmaliaAffiliation unknown1
Firman RezaldiAffiliation unknown1
Ismi Farah SyarifahAffiliation unknown1
Ghea Gema AdzaniAffiliation unknown1
Nisrina HasnaAffiliation unknown1
Opik Taufiqurrahman4Affiliation unknown1
Listia Wati SophiaAffiliation unknown1
Ida KinasihAffiliation unknown1
Rahmat Taufiq Mustahiq AkbarAffiliation unknown1
Amalia KhoirAffiliation unknown1
Poniah AndayaningsihAffiliation unknown1
Ida RohimahAffiliation unknown1
Neng Hilma HamidahAffiliation unknown1
Rahmat Taufiq Mustahiq AkbarAffiliation unknown1
Cahyo RahmadiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, first harvested 5 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">GC-MS Profiles, Anti-inflammatory and Antibacterial Activities of Mucuna pruriens Seed Against Gastrointestinal Pathogens</dc:title>
	<dc:creator xml:lang="en">Musa'adah, Musa'adah </dc:creator>
	<dc:creator xml:lang="en">Suryani, Yani </dc:creator>
	<dc:creator xml:lang="en">Adisty Virakawugi Darniwa</dc:creator>
	<dc:creator xml:lang="en">Mumtazah, Muna </dc:creator>
	<dc:creator xml:lang="en">Burhanuddin, Muhammad Wisnu </dc:creator>
	<dc:creator xml:lang="en">Ependi, Ahmad Zaenal </dc:creator>
	<dc:subject xml:lang="en">B. cereus</dc:subject>
	<dc:subject xml:lang="en">bioactive compounds</dc:subject>
	<dc:subject xml:lang="en">E. coli</dc:subject>
	<dc:subject xml:lang="en">Mucuna pruriens</dc:subject>
	<dc:subject xml:lang="en">red blood cell membrane</dc:subject>
	<dc:description xml:lang="en">Escherichia coli and Bacillus cereus are pathogenic bacteria commonly implicated in gastrointestinal infection (GI). Severe GI can lead to some gastrointestinal diseases, such as diarrhea, gastroenteritis, and chronic inflammation. While antibiotic therapy remains the standard treatment, the rise in antimicrobial resistance (AMR) cases necessitates alternative therapeutic approaches. This study aimed to investigate the antibacterial and anti-inflammatory potential of Velvet bean (Mucuna pruriens L.) and to identify its bioactive compounds using gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was assessed against E. coli and B. cereus using the disc diffusion method. Meanwhile, anti-inflammatory activity was measured by the red blood cell membrane stabilization method at extract concentrations of 15, 60, 250, and 1000 ppm. Antibacterial effectiveness was determined by measuring the diameter of inhibition zones, while anti-inflammatory potential was quantified through stability percentages. The results showed that the velvet bean’s ethanolic extract had antibacterial activity against both bacteria, with the strongest inhibition observed at 100% extract concentration, producing inhibition zones of 9.2 mm on E. coli and 7.15 mm on B. cereus. For the anti-inflammatory assay, the 1000 ppm extract demonstrated the highest stability value (76.36%), nearly equivalent to the standard non-steroidal anti-inflammatory drug (NSAID), ibuprofen (76.35% at 120 ppm), as a positive control. GC-MS analysis revealed the presence of 18 bioactive compounds in the velvet bean extract, classified into nine chemical groups, which are mostly known for their antimicrobial and anti-inflammatory potential. These findings highlight the potential of M. pruriens to address gastrointestinal infections, particularly those caused by E. coli and B. cereus, as well as its anti-inflammatory effect. This also contributes to achieving sustainable development goals (SDGs), especially to encourage good health in the community through utilizing local resources for herbal-based treatment.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2026-04-28</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/33258</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v18i1.33258</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 18 No. 1 (2026): April 2026; 78-92</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v18i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/33258/9209</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2026 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>