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Effect of Temperature and Time on the Sterilization Process of Soft-boned Milkfish

Jurnal Inovasi Mesin · Vol. 6 No. 2 (2024) · pp. 25–32

DOI
10.15294/jim.v6i2.12273
ISSN
2746-7694
Published
2024-10-25
Publisher
Universitas Negeri Semarang
Language
eng

Abstract

The objectives of this research are: 1) Analyzing the effect of sterilization temperature and time on protein content in soft spiny milkfish, 2) Analyzing the effect of sterilization temperature and time on the bacterial content in soft spiny milkfish, and 3) Knowing the ideal temperature and time in the milkfish sterilization process soft spines. There are various types of diversification of processed milkfish, including processing it into presto milkfish. The shelf life of pressure-cooked milkfish can be increased by sterilization using a high-pressure pressure cooker using a retort tool. In this study, the sterilization process used a combination of temperatures of 110°C, 121°C, 130°C, and times of 10, 20, and 30 minutes. The research method uses an experimental approach. The research location is CV Sekar Tekno, Semarang. The data collection technique uses observation, which is carried out by systematically observing and recording the symptoms being investigated. Data analysis techniques use descriptive statistics and analysis of variance/ANOVA. The results of the research showed that the effect of temperature and time on the sterilization process of soft spiny milkfish did not have a significant effect on the protein content of soft spiny milkfish. Although the protein content of soft spiny milkfish experienced changes up and down during the sterilization process, the protein content did not decrease significantly. The effect of high temperature in the sterilization process of soft spiny milkfish has a significant effect on the number of bacteria present in soft spiny milkfish after the sterilization process. This is indicated by the phenomenon of a constant decrease/value of bacterial content as shown in the following data: temperature 110°C is 148.44 CFU/g, temperature 121°C is 86.78 CFU/g, and temperature 130°C is 25.44 CFU/g. On the other hand, the effect of long sterilization time on the bacterial content of soft spiny milkfish tends to show an up-and-down phenomenon. This is indicated by an increasing trend in the number of bacteria at 30 minutes, which was 125 CFU/g. This phenomenon is caused by a leak in the retort pack used for soft spiny milkfish and other causes that are not yet known for certain in this process. Based on the results of the effect of temperature and time on protein levels and bacterial content, it shows that a temperature of 130°C has a high protein content of 16.6% with the lowest bacterial content, namely 45 CFU/g. At 20 minutes, it had a high protein content of 14.296% with the lowest bacterial content, namely 32.67 CFU/g. So the ideal temperature and time for the sterilization process of soft spiny milkfish is 130°C and 20 minutes

Keywords

Collaboration network of the authors

18 authors · 50 co-authorship links

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  • State University of Semarang
  • Affiliation unknown
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Co-authorOrganisationJoint articles
KriswantoAffiliation unknown1
Muhammad Wifaqurrokhman AbdissalamAffiliation unknown1
Heri YudionoAffiliation unknown1
Wirawan SumbodoAffiliation unknown1
Danang Dwi SaputroAffiliation unknown1
Rizki SetiadiAffiliation unknown1
Arif RahmadhiAffiliation unknown1
Febrian Arif BudimanAffiliation unknown1
Angga SeptiyantoAffiliation unknown1
Winarno Dwi RahardjoAffiliation unknown1
Sudiyono SudiyonoAffiliation unknown1
Wahyu Ady Priyo KuncahyoAffiliation unknown1
Bunyamin BunyaminState University of Semarang1
Rizky SetiadiAffiliation unknown1
Agung BudiwirawanAffiliation unknown1
Sofi Alifia HidyusAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/jim/oai, first harvested 6 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc)
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	<dc:title xml:lang="en">Effect of Temperature and Time on the Sterilization Process of Soft-boned Milkfish</dc:title>
	<dc:creator xml:lang="en">Ahmad Tafsirul Hakim Nur Sya'bani</dc:creator>
	<dc:creator xml:lang="en">Musyono, Ari Dwi Nur Indriawan</dc:creator>
	<dc:subject xml:lang="en">Milkfish</dc:subject>
	<dc:subject xml:lang="en">Sterilization</dc:subject>
	<dc:subject xml:lang="en">Temperature</dc:subject>
	<dc:subject xml:lang="en">Time</dc:subject>
	<dc:description xml:lang="en">The objectives of this research are: 1) Analyzing the effect of sterilization temperature and time on protein content in soft spiny milkfish, 2) Analyzing the effect of sterilization temperature and time on the bacterial content in soft spiny milkfish, and 3) Knowing the ideal temperature and time in the milkfish sterilization process soft spines. There are various types of diversification of processed milkfish, including processing it into presto milkfish. The shelf life of pressure-cooked milkfish can be increased by sterilization using a high-pressure pressure cooker using a retort tool. In this study, the sterilization process used a combination of temperatures of 110°C, 121°C, 130°C, and times of 10, 20, and 30 minutes. The research method uses an experimental approach. The research location is CV Sekar Tekno, Semarang. The data collection technique uses observation, which is carried out by systematically observing and recording the symptoms being investigated. Data analysis techniques use descriptive statistics and analysis of variance/ANOVA. The results of the research showed that the effect of temperature and time on the sterilization process of soft spiny milkfish did not have a significant effect on the protein content of soft spiny milkfish. Although the protein content of soft spiny milkfish experienced changes up and down during the sterilization process, the protein content did not decrease significantly. The effect of high temperature in the sterilization process of soft spiny milkfish has a significant effect on the number of bacteria present in soft spiny milkfish after the sterilization process. This is indicated by the phenomenon of a constant decrease/value of bacterial content as shown in the following data: temperature 110°C is 148.44 CFU/g, temperature 121°C is 86.78 CFU/g, and temperature 130°C is 25.44 CFU/g. On the other hand, the effect of long sterilization time on the bacterial content of soft spiny milkfish tends to show an up-and-down phenomenon. This is indicated by an increasing trend in the number of bacteria at 30 minutes, which was 125 CFU/g. This phenomenon is caused by a leak in the retort pack used for soft spiny milkfish and other causes that are not yet known for certain in this process. Based on the results of the effect of temperature and time on protein levels and bacterial content, it shows that a temperature of 130°C has a high protein content of 16.6% with the lowest bacterial content, namely 45 CFU/g. At 20 minutes, it had a high protein content of 14.296% with the lowest bacterial content, namely 32.67 CFU/g. So the ideal temperature and time for the sterilization process of soft spiny milkfish is 130°C and 20 minutes</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-10-25</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/jim/article/view/12273</dc:identifier>
	<dc:identifier>10.15294/jim.v6i2.12273</dc:identifier>
	<dc:source xml:lang="en">Jurnal Inovasi Mesin; Vol. 6 No. 2 (2024): OCTOBER 2024; 25-32</dc:source>
	<dc:source>2746-7694</dc:source>
	<dc:source>10.15294/jim.v6i2</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/jim/article/view/12273/3965</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Jurnal Inovasi Mesin</dc:rights>
</oai_dc:dc>