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Antidiabetic potential of Acacia auriculiformis leaf extract based on antioxidant activity (DPPH assay)

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · pp. 416–423

DOI
10.22437/proca.v1i2.50264
Published
2025-11-26
Publisher
LPPM Universitas Jambi
Language
en
License
creativecommons.org/licenses/by/4.0

Abstract

Background: Diabetes mellitus is a chronic metabolic disorder associated with oxidative stress, which leads to complications in various organs. Natural antioxidants from plants have gained attention as potential antidiabetic agents due to their ability to neutralize reactive oxygen species (ROS). Objective: This study aimed to evaluate the antioxidant potential of Acacia auriculiformis leaf extract as a preliminary indicator of its antidiabetic activity using the DPPH radical scavenging method. Methods: The antioxidant activity was determined by the in vitro DPPH (2,2-diphenyl-1-picrylhydrazyl) assay using methanolic extracts of A. auriculiformis leaves at concentrations of 6–100 ppm. The percentage of inhibition was measured spectrophotometrically at 492 nm, and IC₅₀ values were calculated using nonlinear regression analysis with GraphPad Prism 10.3.1. Results: The extract showed a concentration-dependent increase in DPPH radical inhibition, with an IC₅₀ value of 70.09 ppm, classifying it as a strong antioxidant (IC₅₀ < 100 ppm). The correlation analysis (r = 0.9972, p < 0.001) confirmed a strong linear relationship between concentration and antioxidant activity. Conclusion: These findings suggest that A. auriculiformis leaf extract exhibits strong antioxidant activity, likely attributed to its phenolic, flavonoid, and tannin constituents, supporting its potential as a natural candidate for antidiabetic therapy development.

Keywords

Collaboration network of the authors

40 authors · 227 co-authorship links · showing the 40 most connected

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Co-authorOrganisationJoint articles
Denok Tri HardiningsihAffiliation unknown20
Esa Indah AyudiaAffiliation unknown9
MiftahurrahmahAffiliation unknown8
Afifah AmatullahAffiliation unknown7
Anggelia PuspasariAffiliation unknown6
Citra MaharaniAffiliation unknown5
Rina Nofri EnisAffiliation unknown5
Mara Imam Taufiq SiregarAffiliation unknown5
Wahyu Indah Dewi AuroraAffiliation unknown5
Ahmad Thohir HidayatAffiliation unknown5
Erny KusdiyahAffiliation unknown4
Patrick Wiliam GadingAffiliation unknown4
Fathnur Sani KasmadiAffiliation unknown3
Arnati WulansariAffiliation unknown3
Evy WisudarianiAffiliation unknown3
Nadir Putra Indra TariganAffiliation unknown3
Mirna Marhami IskandarAffiliation unknown3
Armaidi DarmawanAffiliation unknown3
Rita HalimAffiliation unknown3
HaninaAffiliation unknown3
Puspita SariAffiliation unknown2
FairuzAffiliation unknown2
HumaryantoAffiliation unknown2
Hasna DewiAffiliation unknown2
Amelia Dwi FitriAffiliation unknown2
Nyimas Natasha Ayu ShafiraAffiliation unknown2
Widya Lawra ArrahmaAffiliation unknown2
Syahreza Hadi JuandaAffiliation unknown2
Tengku Irfan Wira BuanaAffiliation unknown2
Tia Wida Ekaputri HzAffiliation unknown2
Usi LanitaAffiliation unknown1
Muhammad Rifqi AzharyAffiliation unknown1
willia novitaAffiliation unknown1
Vina Rahayu NingsihAffiliation unknown1
Willia Novita Eka RiniAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, first harvested 6 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Antidiabetic potential of Acacia auriculiformis leaf extract based on antioxidant activity (DPPH assay)</dc:title>
	<dc:creator xml:lang="en">Delfira, Annissa</dc:creator>
	<dc:creator xml:lang="en">Asty, Zahra Frizki</dc:creator>
	<dc:creator xml:lang="en">Perkasa, Tengku Arief Buana</dc:creator>
	<dc:creator xml:lang="en">Hafizah</dc:creator>
	<dc:creator xml:lang="en">Wijayanti, Zilzikridini</dc:creator>
	<dc:subject xml:lang="en">Acacia auriculiformis</dc:subject>
	<dc:subject xml:lang="en">DPPH assay</dc:subject>
	<dc:subject xml:lang="en">antioxidant</dc:subject>
	<dc:subject xml:lang="en">IC₅₀</dc:subject>
	<dc:subject xml:lang="en">natural antidiabetic agent</dc:subject>
	<dc:subject xml:lang="en">ROS (Reactive Oxygen Species)</dc:subject>
	<dc:description xml:lang="en">Background: Diabetes mellitus is a chronic metabolic disorder associated with oxidative stress, which leads to complications in various organs. Natural antioxidants from plants have gained attention as potential antidiabetic agents due to their ability to neutralize reactive oxygen species (ROS). Objective: This study aimed to evaluate the antioxidant potential of Acacia auriculiformis leaf extract as a preliminary indicator of its antidiabetic activity using the DPPH radical scavenging method. Methods: The antioxidant activity was determined by the in vitro DPPH (2,2-diphenyl-1-picrylhydrazyl) assay using methanolic extracts of A. auriculiformis leaves at concentrations of 6–100 ppm. The percentage of inhibition was measured spectrophotometrically at 492 nm, and IC₅₀ values were calculated using nonlinear regression analysis with GraphPad Prism 10.3.1. Results: The extract showed a concentration-dependent increase in DPPH radical inhibition, with an IC₅₀ value of 70.09 ppm, classifying it as a strong antioxidant (IC₅₀ &amp;lt; 100 ppm). The correlation analysis (r = 0.9972, p &amp;lt; 0.001) confirmed a strong linear relationship between concentration and antioxidant activity. Conclusion: These findings suggest that A. auriculiformis leaf extract exhibits strong antioxidant activity, likely attributed to its phenolic, flavonoid, and tannin constituents, supporting its potential as a natural candidate for antidiabetic therapy development.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50264</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50264</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 416-423</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50264/22598</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Annissa Delfira, Zahra Frizki Asty, Tengku Arief Buana Perkasa, Hafizah, Zilzikridini Wijayanti</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>