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Antioxidant activity and secondary metabolite profile of protocorm-like bodies (PLBs) of Dendrobium crumenatum and Dendrobium liniale

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · pp. 841–847

DOI
10.22437/proca.v1i2.50455
Published
2025-11-30
Publisher
LPPM Universitas Jambi
Language
en
License
creativecommons.org/licenses/by/4.0

Abstract

Background: The Dendrobium orchid genus is recognized as a source of secondary metabolites with potential therapeutic applications. Sustainable biomass availability can be addressed through the induction of protocorm-like bodies (PLBs). Objective: This study aims to observe the growth of PLBs, screen secondary metabolites, and compare the antioxidant activity between PLBs extracts and plant extracts. Methods: The growth of PLBs Dendrobium crumenatum and Dendrobium liniale was observed until day 60. Secondary metabolite screening was carried out qualitatively to identify the presence of alkaloids, phenolics, tannins, flavonoids, saponins, steroids, and terpenoids. Antioxidant activity was measured using the DPPH method on PLBs extracts, plant extracts, and ascorbic acid as a positive control, with the determination of the inhibitory concentration 50 (IC50) values. Results: PLB growth was observed optimally on day 60. Phytochemical examination identified the presence of compounds from the alkaloid, phenolic, tannin, flavonoid, saponin, steroid, and terpenoid groups in all samples. Antioxidant test showed that Dendrobium crumenatum PLBs extract had the most vigorous activity among all samples, with an IC50 value of 76.06 ± 0.41 µg/mL (strong category). This value was superior to that of the plant extract of D. crumenatum, at 175.26 ± 0.46 µg/mL. In general, PLB extracts from both species produced significantly more vigorous antioxidant activity than plant extracts. Conclusion: PLB culture is an effective alternative for producing Dendrobium biomass with a higher content of bioactive compounds. D. crumenatum PLB extract has the potential to be developed as a natural antioxidant agent.

Keywords

Collaboration network of the authors

40 authors · 178 co-authorship links · showing the 40 most connected

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  • Universitas Halu Oleo
  • Affiliation unknown
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  • Same organisation
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Co-authorOrganisationJoint articles
FitrianingsihAffiliation unknown14
Indri MahariniAffiliation unknown13
Uce LestariAffiliation unknown5
Puspa Dwi PratiwiAffiliation unknown5
Marizki PondawinataAffiliation unknown5
Fathnur Sani KasmadiAffiliation unknown4
Ummi KalsumAffiliation unknown4
Muhammad SyukriUniversitas Halu Oleo3
Attiya IstariniAffiliation unknown2
KamariyahAffiliation unknown2
Syamsurizal SyamsurizalAffiliation unknown2
Riska Amalya NasutionAffiliation unknown2
Nurul Kamilah SadliAffiliation unknown2
Annisa AndrianiAffiliation unknown2
Afifah AzzahraAffiliation unknown2
Dinda Arum Carolina DewiAffiliation unknown2
Tengku Arief Buana PerkasaAffiliation unknown2
Tia Wida Ekaputri HzAffiliation unknown2
Vinka Lupita SariAffiliation unknown2
Santi PerawatiAffiliation unknown2
Indri MaharniAffiliation unknown2
Nurul KamilahAffiliation unknown2
Mirna Marhami IskandarAffiliation unknown1
. YulianaAffiliation unknown1
Indah Sri Wulan SofyanAffiliation unknown1
Sofia Elisa ApriyantiAffiliation unknown1
Aulia Hafizhah NugrohoAffiliation unknown1
Yulwilla VirginiaAffiliation unknown1
AdhyatmikaAffiliation unknown1
Sekar Ayu PawestriAffiliation unknown1
Andy AmirAffiliation unknown1
Arnild Augina MekarisceAffiliation unknown1
AsparianAffiliation unknown1
Clara Amelia PutriAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, first harvested 6 Nov 2026, last seen 6 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Antioxidant activity and secondary metabolite profile of protocorm-like bodies (PLBs) of Dendrobium crumenatum and Dendrobium liniale</dc:title>
	<dc:creator xml:lang="en">Syamsurizal</dc:creator>
	<dc:creator xml:lang="en">Utami, Diah Tri</dc:creator>
	<dc:creator xml:lang="en">Yuliawati</dc:creator>
	<dc:creator xml:lang="en">Elisma</dc:creator>
	<dc:creator xml:lang="en">Maimum</dc:creator>
	<dc:creator xml:lang="en">Prabowo, Beni Hendro</dc:creator>
	<dc:subject xml:lang="en">Dendrobium; protocorm-like bodies (PLBs); DPPH; antioxidants; secondary metabolites; IC50</dc:subject>
	<dc:description xml:lang="en">Background: The Dendrobium orchid genus is recognized as a source of secondary metabolites with potential therapeutic applications. Sustainable biomass availability can be addressed through the induction of protocorm-like bodies (PLBs). Objective: This study aims to observe the growth of PLBs, screen secondary metabolites, and compare the antioxidant activity between PLBs extracts and plant extracts. Methods: The growth of PLBs Dendrobium crumenatum and Dendrobium liniale was observed until day 60. Secondary metabolite screening was carried out qualitatively to identify the presence of alkaloids, phenolics, tannins, flavonoids, saponins, steroids, and terpenoids. Antioxidant activity was measured using the DPPH method on PLBs extracts, plant extracts, and ascorbic acid as a positive control, with the determination of the inhibitory concentration 50 (IC50) values. Results: PLB growth was observed optimally on day 60. Phytochemical examination identified the presence of compounds from the alkaloid, phenolic, tannin, flavonoid, saponin, steroid, and terpenoid groups in all samples. Antioxidant test showed that Dendrobium crumenatum PLBs extract had the most vigorous activity among all samples, with an IC50 value of 76.06 ± 0.41 µg/mL (strong category). This value was superior to that of the plant extract of D. crumenatum, at 175.26 ± 0.46 µg/mL. In general, PLB extracts from both species produced significantly more vigorous antioxidant activity than plant extracts. Conclusion: PLB culture is an effective alternative for producing Dendrobium biomass with a higher content of bioactive compounds. D. crumenatum PLB extract has the potential to be developed as a natural antioxidant agent.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50455</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50455</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 841-847</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50455/22686</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Syamsurizal, Diah Tri Utami, Yuliawati, Elisma, Maimum, Beni Hendro Prabowo</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
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