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Comparative cytotoxicity and selectivity of various orchid extracts against B16F10 melanoma cells

Proceedings Academic Universitas Jambi · Vol. 1 No. 2 (2025) · pp. 986–993

DOI
10.22437/proca.v1i2.50527
Published
2025-11-30
Publisher
LPPM Universitas Jambi
Language
en
License
creativecommons.org/licenses/by/4.0

Abstract

Background: Melanoma B16F10 is an aggressive form of skin cancer that requires novel therapies. This study aimed to examine the cytotoxicity and selectivity activities of five crude methanol extracts derived from three different orchid species—Cymbidium sp. (leaves), Grammatophyllum speciosum (leaves), and Dendrobium crumenatum (leaves, pseudobulbs, and roots) against melanoma cancer cells B16F10. Methods: Cytotoxicity activities were measured using MTT assay against B16F10 (cancer) and 3T3 (normal fibroblasts) cells to determine IC50 and selectivity index (SI). Results: All extracts exhibited dose-dependent cytotoxicity. The extract of Dendrobium crumenatum pseudobulbs showed the most vigorous activity with an IC50 value against B16F10 of 168.47±2.02 µg/mL. This extract also showed the best SI of 3.79 (IC50 3T3 = 638.73±2.98 µg/mL), which indicates a promising selectivity potential compared to other plant part extracts. Conclusion: Crude methanol extract of Dendrobium crumenatum pseudobulbs is the best candidate as a selective anticancer agent for melanoma and requires further study to isolate its active compounds.

Keywords

Collaboration network of the authors

40 authors · 135 co-authorship links · 2 across different organisations · showing the 40 most connected

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  • Institut Agama Islam Negeri Kendari
  • Universitas Jambi
  • Universitas Teknologi Yogyakarta
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Hendra Dhermawan SitanggangAffiliation unknown6
guspianto guspiantoAffiliation unknown6
Dwi NoerjoediantoAffiliation unknown6
Rd. HalimAffiliation unknown6
FitrianingsihAffiliation unknown6
Indri MahariniAffiliation unknown6
M. RidwanAffiliation unknown5
YuliawatiAffiliation unknown5
Adelina FitriAffiliation unknown4
Elisma .Affiliation unknown4
MaimumAffiliation unknown4
Nakhlatul AisyaAffiliation unknown3
Asparian AsparianAffiliation unknown3
Muldiasman MuldiasmanAffiliation unknown3
Irma HandayaniUniversitas Teknologi Yogyakarta3
Nofrans Eka SaputraAffiliation unknown3
Syamsurizal SyamsurizalAffiliation unknown3
Wahyudin MaguniInstitut Agama Islam Negeri Kendari2
Beti MuluInstitut Agama Islam Negeri Kendari2
Yosi OktarinaAffiliation unknown2
Evy WisudarianiAffiliation unknown2
Helmi Suryani NasutionAffiliation unknown2
Jalius JaliusAffiliation unknown2
Damris MuhammadUniversitas Jambi2
Adriani AdrianiAffiliation unknown2
Erny KusdiyahAffiliation unknown2
Wahyu Indah Dewi AuroraAffiliation unknown2
Amelia Dwi FitriAffiliation unknown2
Anggelia PuspasariAffiliation unknown2
Willia Novita Eka RiniAffiliation unknown2
Adzil Arsyi SabanaInstitut Agama Islam Negeri Kendari1
Eka Rizky SaputraInstitut Agama Islam Negeri Kendari1
Rahmi RahmiInstitut Agama Islam Negeri Kendari1
St NapisaInstitut Agama Islam Negeri Kendari1
Satriyani SatriyaniInstitut Agama Islam Negeri Kendari1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/PROCA/oai, first harvested 6 Nov 2026, last seen 6 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Comparative cytotoxicity and selectivity of various orchid extracts against B16F10 melanoma cells</dc:title>
	<dc:creator xml:lang="en">Syamsurizal</dc:creator>
	<dc:creator xml:lang="en">Utami, Diah Tri</dc:creator>
	<dc:creator xml:lang="en">Subandi, Andi</dc:creator>
	<dc:creator xml:lang="en">Kalsum, Ummi</dc:creator>
	<dc:creator xml:lang="en">Prabowo, Beni Hendro</dc:creator>
	<dc:subject xml:lang="en">Orchid; B16F10; Cytotoxicity; Selectivity; Dendrobium crumenatum</dc:subject>
	<dc:description xml:lang="en">Background: Melanoma B16F10 is an aggressive form of skin cancer that requires novel therapies. This study aimed to examine the cytotoxicity and selectivity activities of five crude methanol extracts derived from three different orchid species—Cymbidium sp. (leaves), Grammatophyllum speciosum (leaves), and Dendrobium crumenatum (leaves, pseudobulbs, and roots) against melanoma cancer cells B16F10. Methods: Cytotoxicity activities were measured using MTT assay against B16F10 (cancer) and 3T3 (normal fibroblasts) cells to determine IC50 and selectivity index (SI). Results: All extracts exhibited dose-dependent cytotoxicity. The extract of Dendrobium crumenatum pseudobulbs showed the most vigorous activity with an IC50 value against B16F10 of 168.47±2.02 µg/mL. This extract also showed the best SI of 3.79 (IC50 3T3 = 638.73±2.98 µg/mL), which indicates a promising selectivity potential compared to other plant part extracts. Conclusion: Crude methanol extract of Dendrobium crumenatum pseudobulbs is the best candidate as a selective anticancer agent for melanoma and requires further study to isolate its active compounds.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-11-30</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
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	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/PROCA/article/view/50527</dc:identifier>
	<dc:identifier>10.22437/proca.v1i2.50527</dc:identifier>
	<dc:source xml:lang="en">Proceedings Academic Universitas Jambi; Vol. 1 No. 2 (2025): The 6th Jambi Medicine and Health Science International Conference (JAMHESIC); 986-993</dc:source>
	<dc:source>10.22437/proca.v1i2</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/PROCA/article/view/50527/22722</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Syamsurizal, Diah Tri Utami, Andi Subandi, Ummi Kalsum, Beni Hendro Prabowo</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
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