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COMPARISON OF THREE METHOD OF DNA EXTRACTION FOR METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan · Vol. 10 No. 4 (2022) · pp. 551–556

DOI
10.22437/jmj.v10i4.21047
ISSN
2580-6874, 2339-269X
Published
2022-11-29
Publisher
FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI
Language
en

Abstract

Background: MRSA is a gram positive bacteria that has a peptidoglycan in the cell wall which is noteasy to lysis during the DNA extraction procces.Objectives: This study aimed to compare three DNA extraction method, using a conventional method, chelex method, and commercial kit in MRSA.Methods: This is a descriptive study with laboratory experiments. The sample was swabs of purulent wounds from inpatients in Jambi that were randomly selected. Swab sample identified as MRSA was extracted with three methods and tested using UV-Vis spectrophotometer.Results: The study results showed that the best DNA purity was found by DNA extraction usingcommercial kit (1.43), followed by chelex (1.14) and conventional (1.07) methods. Likewise, the highest concentration of DNA was DNA extraction using commercial kit (330 g/ml), followed by the chelex (78 g/ml) and conventional (54 g/ml) methods.Conclusion: A conventional method can be used as an extraction method that is easy to do and more economical. The best DNA purity and concentration was obtained in the kit extraction method, followed by chelex and conventional methods.Keywords: MRSA, extraction, conventional, chelex, kit

Collaboration network of the authors

36 authors · 182 co-authorship links

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Co-authorOrganisationJoint articles
Ahmad SyauqyAffiliation unknown3
Miftahurrahma MiftahurrahmaAffiliation unknown2
Putri Sari WulandariAffiliation unknown2
Tia Wida Ekaputri HzAffiliation unknown2
Hasna DewiAffiliation unknown2
Lipinwati LipinwatiAffiliation unknown2
Huntari HarahapAffiliation unknown2
Wahyu Indah Dewi AuroraAffiliation unknown2
Amelia Dwi FitriAffiliation unknown1
Ulfadiya PutriAffiliation unknown1
Ima MariaAffiliation unknown1
Raihanah SuzanAffiliation unknown1
Ave Olivia RahmanAffiliation unknown1
Budi JustisiaAffiliation unknown1
Esa Indah AyudiaAffiliation unknown1
Tia Wida EkaputriAffiliation unknown1
Budi AswinAffiliation unknown1
Indri MahariniAffiliation unknown1
FitriyantiAffiliation unknown1
Riska Amalya NasutionAffiliation unknown1
Rion NofriandaAffiliation unknown1
Puspa Dwi PuspitaAffiliation unknown1
Nofrans Eka PutraAffiliation unknown1
Ummi KalsumAffiliation unknown1
Maudy Rominar Br TobingAffiliation unknown1
Rita HalimAffiliation unknown1
Muhammad FadliAffiliation unknown1
Budi JustitiaAffiliation unknown1
Lipinwati HusmanAffiliation unknown1
Fairuz FairuzAffiliation unknown1
Attiya IstariniAffiliation unknown1
Mirna Marhami IskandarAffiliation unknown1
Melly MirandaAffiliation unknown1
Rianita JuniatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/kedokteran/oai, first harvested 6 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">COMPARISON OF THREE METHOD OF DNA EXTRACTION FOR METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)</dc:title>
	<dc:creator xml:lang="en">Hanina, Hanina</dc:creator>
	<dc:creator xml:lang="en">Humaryanto, Humaryanto</dc:creator>
	<dc:description xml:lang="en"> Background: MRSA is a gram positive bacteria that has a peptidoglycan in the cell wall which is noteasy to lysis during the DNA extraction procces.Objectives: This study aimed to compare three DNA extraction method, using a conventional method, chelex method, and commercial kit in MRSA.Methods: This is a descriptive study with laboratory experiments. The sample was swabs of purulent wounds from inpatients in Jambi that were randomly selected. Swab sample identified as MRSA was extracted with three methods and tested using UV-Vis spectrophotometer.Results: The study results showed that the best DNA purity was found by DNA extraction usingcommercial kit (1.43), followed by chelex (1.14) and conventional (1.07) methods. Likewise, the highest concentration of DNA was DNA extraction using commercial kit (330 g/ml), followed by the chelex (78 g/ml) and conventional (54 g/ml) methods.Conclusion: A conventional method can be used as an extraction method that is easy to do and more economical. The best DNA purity and concentration was obtained in the kit extraction method, followed by chelex and conventional methods.Keywords: MRSA, extraction, conventional, chelex, kit </dc:description>
	<dc:publisher xml:lang="en">FAKULTAS KEDOKTERAN DAN ILMU KESEHATAN UNIVERSITAS JAMBI</dc:publisher>
	<dc:date>2022-11-29</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/kedokteran/article/view/21047</dc:identifier>
	<dc:identifier>10.22437/jmj.v10i4.21047</dc:identifier>
	<dc:source xml:lang="en">Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan; Vol. 10 No. 4 (2022): Jambi Medical Journal: Jurnal Kedokteran dan Kesehatan ; 551-556</dc:source>
	<dc:source>2580-6874</dc:source>
	<dc:source>2339-269X</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/kedokteran/article/view/21047/14402</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 JAMBI MEDICAL JOURNAL "Jurnal Kedokteran dan Kesehatan"</dc:rights>
</oai_dc:dc>