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Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers

HAYATI Journal of Biosciences · Vol. 14 No. 2 (2009)

DOI
10.4308/hjb.14.2.76
ISSN
2086-4094, 1978-3019
Terbit
2009-12-02
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer

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  • Universitas Halu Oleo
  • IPB University
  • Hasanuddin University
  • Universitas Sumatera Utara
  • National Research and Innovation Agency
  • University of Indonesia
  • Universitas Pattimura
  • Organisasi lain
  • Afiliasi tidak diketahui
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Rekan penulisOrganisasiArtikel bersama
SRI WAHYUNIHasanuddin University9
Asmar HasanUniversitas Halu Oleo6
La Ode Santiaji BandeUniversitas Halu Oleo5
Vit Neru SatrahUniversitas Halu Oleo5
Gusti Ayu Kade SutariatiUniversitas Halu Oleo5
ABDUL RAHMANUniversitas Halu Oleo5
Esti PuspitasariIPB University4
İman RusmanaIPB University4
Nisa Rachmania MubarikIPB University4
TERRY PAKKIUniversitas Halu Oleo3
Muhammad TaufikUniversitas Halu Oleo3
Gusnawaty HSUniversitas Halu Oleo3
Muhammad TaufikUniversitas Halu Oleo3
Dwi SuryantoUniversitas Sumatera Utara3
Anja MeryandiniIPB University3
Aris Tri WahyudiIPB University3
Andreas Adhi SatyaAfiliasi tidak diketahui3
GiyantoNational Research and Innovation Agency3
MARIADI MARIADIUniversitas Halu Oleo2
SYAIR SYAIRUniversitas Halu Oleo2
Rahayu MallarangengUniversitas Halu Oleo2
Asniah AsniahUniversitas Halu Oleo2
Teguh WijayantoUniversitas Halu Oleo2
HS GusnawatyAfiliasi tidak diketahui2
Abdul RahmanUniversitas Halu Oleo2
Nizirwan AnwarAfiliasi tidak diketahui2
Widanarni WidanarniIPB University2
Amarila MalikUniversity of Indonesia2
Bibiana W. LayIPB University2
Yulin LestariIPB University2
Dedy Duryadi SolihinIPB University2
Cecilia Anna SeumahuUniversitas Pattimura2
Maria Indah PurnamasariMontana State University2
Rika Indri AstutiIPB University2
Yepy H. RustamAfiliasi tidak diketahui2
Haliatur RahmaAndalas University2

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers</dc:title>
	<dc:creator xml:lang="en">KHAERUNI, ANDI</dc:creator>
	<dc:creator xml:lang="en">SUWANTO, ANTONIUS</dc:creator>
	<dc:creator xml:lang="en">TJAHJONO, BUDI</dc:creator>
	<dc:creator xml:lang="en">SINAGA, MEITY SURADJI</dc:creator>
	<dc:description xml:lang="en">A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer </dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2009-12-02</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/243</dc:identifier>
	<dc:identifier>10.4308/hjb.14.2.76</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 14 No. 2 (2007): June 2007; 76</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/243/109</dc:relation>
</oai_dc:dc>