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Haemozoin Detection in Mouse Liver Histology Using Simple Polarized Light Microscope

HAYATI Journal of Biosciences · Vol. 21 No. 1 (2014) · hlm. 48–52

DOI
10.4308/hjb.21.1.48-52
ISSN
2086-4094, 1978-3019
Terbit
2014-03-26
Penerbit
Bogor Agricultural University, Indonesia
Bahasa
eng

Abstrak

The presence of malarial pigment (haemozoin) due to Plasmodium infection is a common histopathological effect in mouse liver. Previous research showed that by using a polarized light microscope, researchers were better able to detect haemozoin in mouse liver histology section. Thus, the aim of this research was to compare the haemozoin area observed by a conventional vs. simple polarized light microscope by using image processing analysis. A total of 40 images produced from both conventional light microscope and simple polarized light microscope were collected. All images were analyzed using ImageJ 1.47 software to measure the haemozoin areas. Our results showed that non birefringent haemozoin and birefringent haemozoin area was significantly different. This was because when using conventional light microscope the brown area that contained images of non birefringent haemozoin images also contained Kupffer cells which appeared as the same brown color as haemozoin. In contrast, haemozoin gave bright effect and can be easily differentiated with Kupffer cells in the birefringent haemozoin images. This study concluded that haemozoin detection in mouse liver histology using a simple polarized light microscope was more accurate compared to that of conventional light microscope.

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  • Universitas Halu Oleo
  • Institut Agama Islam Negeri Kendari
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Rekan penulisOrganisasiArtikel bersama
Muhammad Amrullah PagalaUniversitas Halu Oleo1
La Ode SahabaUniversitas Halu Oleo1
La Ode Arsad SaniUniversitas Halu Oleo1
Surahmanto SurahmantoAfiliasi tidak diketahui1
Hairil A HadiniAfiliasi tidak diketahui1
Nurjannah NurjannahInstitut Agama Islam Negeri Kendari1
Makmur KambolongUniversitas Halu Oleo1
Nyoman Ningsih UtamiAfiliasi tidak diketahui1
Rudi HartonoAfiliasi tidak diketahui1
Rahayu Puji HAfiliasi tidak diketahui1
Kardi KardiAfiliasi tidak diketahui1
Raden IndarjaniAfiliasi tidak diketahui1
Yayuk AstutiAfiliasi tidak diketahui1
Hendri WidiyandariAfiliasi tidak diketahui1
Arnelli ArnelliAfiliasi tidak diketahui1
Pardoyo PardoyoAfiliasi tidak diketahui1
Amilia FauziyahAfiliasi tidak diketahui1
Arum Dista WulansariAfiliasi tidak diketahui1
Rizka AndianingrumAfiliasi tidak diketahui1
Gaurav A. BhaduriAfiliasi tidak diketahui1
SOFIATI PURNAMIAfiliasi tidak diketahui1
Muhammad AriyonAfiliasi tidak diketahui1
Ellyan SastraningsihAfiliasi tidak diketahui1
Putri RahmatillahAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc)
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	<dc:title xml:lang="en">Haemozoin Detection in Mouse Liver Histology Using Simple Polarized Light Microscope</dc:title>
	<dc:creator xml:lang="en">RAMADHANI, DWI</dc:creator>
	<dc:creator xml:lang="en">NURHAYATI, SITI</dc:creator>
	<dc:creator xml:lang="en">RAHARDJO, TUR</dc:creator>
	<dc:subject xml:lang="en">haemozoin</dc:subject>
	<dc:subject xml:lang="en">liver histology</dc:subject>
	<dc:subject xml:lang="en">mouse</dc:subject>
	<dc:subject xml:lang="en">microscope</dc:subject>
	<dc:subject xml:lang="en">polarization</dc:subject>
	<dc:description xml:lang="en">The presence of malarial pigment (haemozoin) due to Plasmodium infection is a common histopathological effect in mouse liver. Previous research showed that by using a polarized light microscope, researchers were better able to detect haemozoin in mouse liver histology section. Thus, the aim of this research was to compare the haemozoin area observed by a conventional vs. simple polarized light microscope by using image processing analysis. A total of 40 images produced from both conventional light microscope and simple polarized light microscope were collected. All images were analyzed using ImageJ 1.47 software to measure the haemozoin areas. Our results showed that non birefringent haemozoin and birefringent haemozoin area was significantly different. This was because when using conventional light microscope the brown area that contained images of non birefringent haemozoin images also contained Kupffer cells which appeared as the same brown color as haemozoin. In contrast, haemozoin gave bright effect and can be easily differentiated with Kupffer cells in the birefringent haemozoin images. This study concluded that haemozoin detection in mouse liver histology using a simple polarized light microscope was more accurate compared to that of conventional light microscope.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2014-03-26</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/7730</dc:identifier>
	<dc:identifier>10.4308/hjb.21.1.48-52</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 21 No. 1 (2014): March 2014; 48-52</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/7730/pdf</dc:relation>
</oai_dc:dc>