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Media Manipulation to Enhance In Vitro Pitcher Formation in Nepenthes mirabilis (Lour.) Druce for Microfloriculture Development

Caraka Tani: Journal of Sustainable Agriculture · Vol. 40 No. 3 (2025) · hlm. 361–375

DOI
10.20961/carakatani.v40i3.101139
ISSN
2599-2570, 2613-9456
Terbit
2025-06-24
Penerbit
Universitas Sebelas Maret
Bahasa
eng
Lisensi
creativecommons.org/licenses/by-nc/4.0

Abstrak

Microfloriculture refers to souvenirs and Living Room Ornaments made from the in vitro cultured ornamental plants, which are innovative, unique, environmentally friendly, and can be produced across the year. In vitro pitcher-forming Nepenthes mirabilis will increase the attractiveness and market value of microfloriculture products. Two experiments have been carried out to stimulate in vitro pitcher formation in N. mirabilis using a completely randomized design (CRD) of a two-factorial treatment pattern with three replicates. The first experiment tested different Murashige and Skoog (MS) media formulations (full-strength, 3/4MS, 1/2MS, and 1/4MS) and phytagel concentrations (0, 2.5, 3.5, and 4.5 g l-1). The same media formulations and 6-Benzylaminopurine (BAP) concentrations (0, 0.5, 1.0, and 1.5 μM) were tested in the second experiment. The results showed that the explants cultured on 1/4MS media produced the greatest number of shoots (3.83-8.25) and leaves (26.17-26.75), the earliest pitcher formation (27.75±2.06 days), the highest number of pitchers (6.50±2.65), and pitcher-forming leaves (23.30±5.10%). Liquid media enhanced leaves (28.33±4.97) and pitchers (6.42±3.36) formation as well as pitcher-forming leaves percentage (21.37±8.01%). A 1/4MS supplemented with 0.5 μM BAP maximized pitcher-forming leaves (21.08±7.78%) and pitcher number (5.67±2.52). Adding 1.5 μM BAP on 1/4MS media produced the highest number of leaves (38.67±5.69). Full-strength MS produced the lowest number of shoots, leaves, pitchers, and pitcher-forming leaves. This study developed an affordable and effective method to improve in vitro pitcher development in N. mirabilis, highlighting how nutrient levels and BAP concentration influence pitcher formation, which aids in optimizing culture techniques for ornamental carnivorous plants.

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  • Jenderal Soedirman University
  • Universitas Gadjah Mada
  • Sebelas Maret University
  • University of Sheffield
  • State University of Semarang
  • The University of Kitakyushu
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Rekan penulisOrganisasiArtikel bersama
Prita Sari DewiJenderal Soedirman University2
Aqib Ossa Eldurr IftitahAfiliasi tidak diketahui1
Yulias Ninik WindriyatiAfiliasi tidak diketahui1
Adolf Z.D SiahayAfiliasi tidak diketahui1
Simson WerimonAfiliasi tidak diketahui1
Masrukan MasrukanState University of Semarang1
Wahyu HardyantoAfiliasi tidak diketahui1
SugiyonoJenderal Soedirman University1
Allya Alifia PurbayaJenderal Soedirman University1
Siti SamiyarsihJenderal Soedirman University1
Bambang Tejo PremonoAfiliasi tidak diketahui1
Djati Waluyo DjoarSebelas Maret University1
Panut SahariSebelas Maret University1
Bart Julien DewanckerThe University of Kitakyushu1
Tri UntariAfiliasi tidak diketahui1
Yuli Purwandari KristianingrumUniversitas Gadjah Mada1
Sitarina WidyariniUniversitas Gadjah Mada1
Sugi WinarsihAfiliasi tidak diketahui1
Bambang SutrisnoUniversitas Gadjah Mada1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jurnal.uns.ac.id/carakatani/oai, pertama di-harvest 8 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en-US">Media Manipulation to Enhance In Vitro Pitcher Formation in Nepenthes mirabilis (Lour.) Druce for Microfloriculture Development</dc:title>
	<dc:creator>Sugiyono, Sugiyono</dc:creator>
	<dc:creator>Tyas, Anindya Puspaning</dc:creator>
	<dc:creator>Indikasuri, Dea Yuniar</dc:creator>
	<dc:creator>Lestari, Sri</dc:creator>
	<dc:creator>Davison, Paul A</dc:creator>
	<dc:creator>Prasetyo, Rendie</dc:creator>
	<dc:subject xml:lang="en-US">Biology; Plant In Vitro Culture</dc:subject>
	<dc:subject xml:lang="en-US">BAP; media formulation; microfloriculture; Nepenthes mirabilis; pitcher formation</dc:subject>
	<dc:description xml:lang="en-US">Microfloriculture refers to souvenirs and Living Room Ornaments made from the in vitro cultured ornamental plants, which are innovative, unique, environmentally friendly, and can be produced across the year. In vitro pitcher-forming Nepenthes mirabilis will increase the attractiveness and market value of microfloriculture products. Two experiments have been carried out to stimulate in vitro pitcher formation in N. mirabilis using a completely randomized design (CRD) of a two-factorial treatment pattern with three replicates. The first experiment tested different Murashige and Skoog (MS) media formulations (full-strength, 3/4MS, 1/2MS, and 1/4MS) and phytagel concentrations (0, 2.5, 3.5, and 4.5 g l-1). The same media formulations and 6-Benzylaminopurine (BAP) concentrations (0, 0.5, 1.0, and 1.5 μM) were tested in the second experiment. The results showed that the explants cultured on 1/4MS media produced the greatest number of shoots (3.83-8.25) and leaves (26.17-26.75), the earliest pitcher formation (27.75±2.06 days), the highest number of pitchers (6.50±2.65), and pitcher-forming leaves (23.30±5.10%). Liquid media enhanced leaves (28.33±4.97) and pitchers (6.42±3.36) formation as well as pitcher-forming leaves percentage (21.37±8.01%). A 1/4MS supplemented with 0.5 μM BAP maximized pitcher-forming leaves (21.08±7.78%) and pitcher number (5.67±2.52). Adding 1.5 μM BAP on 1/4MS media produced the highest number of leaves (38.67±5.69). Full-strength MS produced the lowest number of shoots, leaves, pitchers, and pitcher-forming leaves. This study developed an affordable and effective method to improve in vitro pitcher development in N. mirabilis, highlighting how nutrient levels and BAP concentration influence pitcher formation, which aids in optimizing culture techniques for ornamental carnivorous plants.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Sebelas Maret</dc:publisher>
	<dc:contributor xml:lang="en-US">Directorate General of Higher Education, Research and Technology, Indonesian Ministry of Education, Culture, Research, and Technology for funding through a Matching Fund Grant (2022-2023)</dc:contributor>
	<dc:date>2025-06-24</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US"></dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jurnal.uns.ac.id/carakatani/article/view/101139</dc:identifier>
	<dc:identifier>10.20961/carakatani.v40i3.101139</dc:identifier>
	<dc:source xml:lang="en-US">Caraka Tani: Journal of Sustainable Agriculture; Vol 40, No 3 (2025): July; 361-375</dc:source>
	<dc:source>2599-2570</dc:source>
	<dc:source>2613-9456</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/view/101139/pdf</dc:relation>
	<dc:relation>https://jurnal.uns.ac.id/carakatani/article/downloadSuppFile/101139/21045</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2025 Caraka Tani: Journal of Sustainable Agriculture</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
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