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Group A β-hemolytic Streptococcus Detection Using Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG)

Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences · Vol. 7 No. 1 (2017) · hlm. 8–15

DOI
10.11594/jtls.07.01.02
ISSN
2527-4376, 2087-5517
Terbit
2017-01-31
Penerbit
Journal of Tropical Life Science
Bahasa
eng

Abstrak

Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria.Â

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  • University of Brawijaya
  • State University of Semarang
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Sigit PriatmokoState University of Semarang1
Catur HerisonAfiliasi tidak diketahui1
Rustikawati RustikawatiAfiliasi tidak diketahui1
Merakati HandayaningsihAfiliasi tidak diketahui1
IS HELIANTIAfiliasi tidak diketahui1
Agustin Krisna WardaniAfiliasi tidak diketahui1
Tri Yudani Mardining RarasAfiliasi tidak diketahui1
Mauludy Jutta AjrullahAfiliasi tidak diketahui1
Ellen Fenix GunawanAfiliasi tidak diketahui1
Nadya Shafa PramestiAfiliasi tidak diketahui1
Maretha HamidaAfiliasi tidak diketahui1
Elfi Anis SaatiAfiliasi tidak diketahui1
Brian Fo’era-era DaelyAfiliasi tidak diketahui1
Susanto NugrohoAfiliasi tidak diketahui1
Rinaldi LengganaAfiliasi tidak diketahui1
Aulanni’am Aulanni’amAfiliasi tidak diketahui1
Tinny Endang HernowatiAfiliasi tidak diketahui1
Avin Ainur FitrianingsihAfiliasi tidak diketahui1
Lailia Nur RachmaAfiliasi tidak diketahui1
Alvi MillianaAfiliasi tidak diketahui1
Sanarto SantosoAfiliasi tidak diketahui1
Umi KalsumAfiliasi tidak diketahui1
Bambang RahardjoAfiliasi tidak diketahui1
Siti Candra Windu BaktiyaniAfiliasi tidak diketahui1
Sultanah ZahariahAfiliasi tidak diketahui1
Tatit NursetaAfiliasi tidak diketahui1
Riny NatalinaAfiliasi tidak diketahui1
Erni YudaningtyasUniversity of Brawijaya1
Edi WidjajantoAfiliasi tidak diketahui1
Mas MansyurAfiliasi tidak diketahui1
Nashi WidodoUniversity of Brawijaya1
Oktavia Rahayu AdianingsihAfiliasi tidak diketahui1
Valentina YurinaAfiliasi tidak diketahui1
Suryanata KesumaAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) http://jtrolis.ub.ac.id/index.php/jtrolis/oai, pertama di-harvest 9 Nov 2026, terakhir terlihat 9 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Group A β-hemolytic Streptococcus Detection Using  Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG)</dc:title>
	<dc:creator xml:lang="en">Alluza, Hamid Hunaif Dhofi</dc:creator>
	<dc:creator xml:lang="en">Mayasari, Ema Dianita</dc:creator>
	<dc:creator xml:lang="en">Prawiro, Sumarno Reto</dc:creator>
	<dc:creator xml:lang="en">Winarsih, Sri</dc:creator>
	<dc:subject xml:lang="en">Streptococcus Group A β-hemolytic</dc:subject>
	<dc:subject xml:lang="en">33 kDa OMP</dc:subject>
	<dc:subject xml:lang="en">IgG anti-33 kDa OMP</dc:subject>
	<dc:description xml:lang="en">Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p &amp;lt; 0.05). The result showed that there was a difference in humoral immune response (IgG) between the groups albeit the difference was not significant (p &amp;gt; 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria. </dc:description>
	<dc:publisher xml:lang="en">Journal of Tropical Life Science</dc:publisher>
	<dc:date>2017-01-31</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/522</dc:identifier>
	<dc:identifier>10.11594/jtls.07.01.02</dc:identifier>
	<dc:source xml:lang="en">Journal of Tropical Life Science; Vol. 7 No. 1 (2017); 8-15</dc:source>
	<dc:source>2527-4376</dc:source>
	<dc:source>2087-5517</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/522/pdf</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2017 Journal of Tropical Life Science</dc:rights>
</oai_dc:dc>