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Kualitas Air Sumur Bor dan Air Swadaya Berdasarkan Total Coliform dengan Cemaran Escherichia coli di Desa Rarowatu Kabupaten Bombana

BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) · Vol. 6 No. 1 (2019) · pp. 911–918

DOI
10.33772/biowallacea.v6i1.8745
ISSN
2685-6360, 2355-6404
Published
2019-09-26
Publisher
University of Halu Oleo
Language
eng
License
creativecommons.org/licenses/by/4.0

Abstract

Penelitian ini bertujuan untuk mengetahui kualitas Air di Desa Rarowatu Kabupaten Bombana. Pemeriksaan coliform pada air sumur bor dan air swadaya masyarakat menggunakan Metode Most Probable Number (MPN). Pada perlakuan ini terdiri dari presumptive test yang menggunakan media Lactose Broth (LB) dengan metode 3 seri tabung, Tahap berikutnya confirmative test dengan menggunakan media Brillian Green Lactose Broth (BGLB). Pada identifikasi Escherichia coli menggunakan media Eosin Methylene Blue Agar, pewarnaan Gram dan media uji biokimia. Hasil penelitian menunjukkan sampel yang terdiri dari 7 sampel air sumur bor dan I sampel air swadaya masyarakat berdasarkan uji MPN mencapai 2400/100 ml. Hasil identifikasi menggunakan media EMBA menunjukkan koloni yang berwarna hijau metalik pada sampel air swadaya serta dilakukan pewarnaan Gram menunjukkan bentuk basil pendek dan gram negative. Hasil uji biokimia menunjukkan hasil uji indol positif, methyl red positif, voges proskauer negatif dan uji sitrat negatif. Uji IMViC dapat disimpulkan bahwa bakteri pada kode sampel D memiliki sifat yang sama terhadap bakteri E. coli. Kata kunci: Coliform, Identifikasi, Escherichia coli, BombanaABSTRACTThis study aims to determine the quality of water in Rarowatu Village, Bombana Regency. Coliform examination in borehole water and community self-water using the Most Probable Number (MPN) Method. This treatment consists of a presumptive test that uses Lactose Broth (LB) media with the tube 3 series method. The next stage is the confirmative test using Brilliant Green Lactose Broth (BGLB) media. The identification of Escherichia coli using Eosin Methylene Blue Agar media, Gram staining, and biochemical test media. The results of the study showed that samples consisting of 7 samples of bore well water and I community self-water samples based on the MPN test reached 2400/100 ml. The identification results using EMBA media showed that metallic green colonies in self-help water samples and Gram staining performed showed short and gram-negative bacilli forms. The results of biochemical tests showed that swadaya water samples were Escherichia coli species.Keywords: Coliform, Identification, Escherichia coli, Bombana

Collaboration network of the authors

11 authors · 17 co-authorship links

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  • Institut Agama Islam Negeri Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Abdul HalimInstitut Agama Islam Negeri Kendari1
Dewi AtikahInstitut Agama Islam Negeri Kendari1
Sucitra Juliana EmsilInstitut Agama Islam Negeri Kendari1
Waode NurcahyanaAffiliation unknown1
Anggriani FusvitaAffiliation unknown1
irin aprilin janharAffiliation unknown1
Firdayanti FirdayantiAffiliation unknown1
Sernita SernitaAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/wallacea/oai, first harvested 31 Okt 2026, last seen 31 Okt 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Kualitas Air Sumur Bor dan Air Swadaya  Berdasarkan Total Coliform dengan Cemaran Escherichia coli di Desa Rarowatu Kabupaten Bombana</dc:title>
	<dc:creator>Fusvita, Angriani</dc:creator>
	<dc:creator>Susanti, Susanti</dc:creator>
	<dc:creator>Anggriawan, Bobi</dc:creator>
	<dc:description xml:lang="en-US">Penelitian ini bertujuan untuk mengetahui kualitas Air di Desa Rarowatu Kabupaten Bombana. Pemeriksaan coliform pada air sumur bor dan air swadaya masyarakat menggunakan Metode Most Probable Number (MPN). Pada perlakuan ini terdiri dari presumptive test yang menggunakan media Lactose Broth (LB) dengan metode 3 seri tabung, Tahap berikutnya confirmative test dengan menggunakan media Brillian Green Lactose Broth (BGLB). Pada identifikasi  Escherichia coli  menggunakan media Eosin Methylene Blue Agar, pewarnaan Gram dan media uji biokimia. Hasil penelitian menunjukkan sampel yang terdiri dari 7 sampel air sumur bor dan I sampel air swadaya masyarakat berdasarkan uji MPN mencapai 2400/100 ml. Hasil identifikasi menggunakan media EMBA menunjukkan koloni yang berwarna hijau metalik pada sampel air swadaya  serta  dilakukan pewarnaan Gram menunjukkan bentuk basil pendek dan gram negative. Hasil uji biokimia menunjukkan hasil uji indol positif, methyl red positif, voges proskauer negatif dan uji sitrat negatif. Uji IMViC dapat disimpulkan bahwa bakteri pada kode sampel D memiliki sifat yang sama terhadap bakteri E. coli. Kata kunci :  Coliform, Identifikasi, Escherichia coli, BombanaABSTRACTThis study aims to determine the quality of water in Rarowatu Village, Bombana Regency. Coliform examination in borehole water and community self-water using the Most Probable Number (MPN) Method. This treatment consists of a presumptive test that uses Lactose Broth (LB) media with the tube 3 series method. The next stage is the confirmative test using Brilliant Green Lactose Broth (BGLB) media. The identification of Escherichia coli using Eosin Methylene Blue Agar media, Gram staining, and biochemical test media. The results of the study showed that samples consisting of 7 samples of bore well water and I community self-water samples based on the MPN test reached 2400/100 ml. The identification results using EMBA media showed that metallic green colonies in self-help water samples and Gram staining performed showed short and gram-negative bacilli forms. The results of biochemical tests showed that swadaya water samples were Escherichia coli species.Keywords: Coliform, Identification, Escherichia coli, Bombana</dc:description>
	<dc:publisher xml:lang="en-US">University of Halu Oleo</dc:publisher>
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	<dc:date>2019-09-26</dc:date>
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	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
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	<dc:identifier>https://ojs.uho.ac.id/index.php/wallacea/article/view/%238745</dc:identifier>
	<dc:identifier>10.33772/biowallacea.v6i1.8745</dc:identifier>
	<dc:source xml:lang="en-US">BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research); Vol 6, No 1 (2019): Biology and Wallacea; 911-918</dc:source>
	<dc:source>2685-6360</dc:source>
	<dc:source>2355-6404</dc:source>
	<dc:source>10.33772/biowallacea.v6i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/wallacea/article/view/%238745/pdf</dc:relation>
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	<dc:rights xml:lang="en-US">Copyright (c) 2019 BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by/4.0</dc:rights>
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