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PERBANYAKAN BIBIT JERUK SECARA IN-VITRO DAN BEBAS PENYAKIT CVPD

Jurnal Agroteknos · Vol. 1 No. 3 (2023)

DOI
10.56189/ja.v1i3.2249
ISSN
2527-4074
Published
2023-06-09
Publisher
Fakultas Pertanian Universitas Halu Oleo
Language
eng

Abstract

Abstract Citrus Vein Phloem Degeneration (CVPD) is one of the most important diseases of citrus. Worldwide and national yield losses of citrus production due to this disease infection have been very significant. This research ultimately aimed at producing citrus stocks through in-vitro culture, and free of CVPD. Citrus seeds were used as explant and cultured on MS and WPM in-vitro media, supplemented with malt extract. Citrus plantlets were tested for the presence of CVPD DNA using CVPD specific primers in PCR reactions. Research results showed that MS basal medium supplemented with malt extract was quite good for in- vitro production of citrus plantlets. Plantlets were negative for CVPD infection based on PCR tests. CVPD-free seedlings (Citrus reticulata) have been grafted with citrus rootstock (Citrus sinensis). Grafted citrus seedlings were also proven to be negative for CVPD infection based on similar PCR tests. Citrus seedlings/stocks produced by in-vitro culture, and free of CVPD, are now available for further growth. Keywords: Citrus Vein Phloem Degeneration (CVPD), in-vitro culture, PCR

Collaboration network of the authors

40 authors · 175 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Halu Oleo
  • Universitas Muhammadiyah Kendari
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Andi KhaeruniUniversitas Halu Oleo8
HS GusnawatyAffiliation unknown7
Asniah AsniahUniversitas Halu Oleo7
Hamirul HadiniUniversitas Halu Oleo5
Asmar HasanUniversitas Halu Oleo4
Gusnawaty HSUniversitas Halu Oleo3
MUHIDIN MUHIDINUniversitas Halu Oleo3
SYAIR SYAIRUniversitas Halu Oleo3
La Ode Arfan DeduAffiliation unknown3
Vit Neru SatrahUniversitas Halu Oleo3
Waode Siti Anima HiseinUniversitas Halu Oleo3
Gusti Ayu Kade SutariatiUniversitas Halu Oleo3
WAODE SITI ANIMA HISEINAffiliation unknown3
MIRZA ARSIATY ARSYADUniversitas Halu Oleo3
RAHAYU MAffiliation unknown2
I GUSTI RAY SADIMANTARAUniversitas Halu Oleo2
Muhammad BotekUniversitas Halu Oleo2
Rahayu M Rahayu MAffiliation unknown2
Gusnawaty HS Gusnawaty HSAffiliation unknown2
Isalman IsalmanAffiliation unknown2
La HataniAffiliation unknown2
Nini Mila RahniUniversitas Halu Oleo2
Anima HiseinAffiliation unknown2
Yusuf YusufUniversitas Muhammadiyah Kendari2
Muhammad MasriAffiliation unknown2
Sarawa MammaUniversitas Halu Oleo2
La Ode SafuanUniversitas Halu Oleo2
Nini Mila RahniAffiliation unknown1
Gusti Ayu Kadek SutariatiAffiliation unknown1
Zulfikar ZulfikarAffiliation unknown1
Rachmi Hariaty HasanAffiliation unknown1
AGUNG YUSWANAUniversitas Halu Oleo1
SUBHAN FITRATUL HAQAffiliation unknown1
NUR ISNAINI ULFAAffiliation unknown1
Muhammad SafarrudinUniversitas Halu Oleo1
Ahmad SainulAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/agroteknos/oai, first harvested 31 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">PERBANYAKAN BIBIT JERUK SECARA IN-VITRO DAN BEBAS PENYAKIT CVPD</dc:title>
	<dc:creator>Wijayanto, Teguh</dc:creator>
	<dc:creator>Taufik, Muhammad</dc:creator>
	<dc:creator>Boer, Dirvamena</dc:creator>
	<dc:creator>Suliartini, Ni Wayan Sri</dc:creator>
	<dc:description xml:lang="en-US">Abstract Citrus Vein  Phloem Degeneration (CVPD) is  one  of  the  most important diseases of citrus. Worldwide and national yield losses of citrus production due to this disease infection have been very significant.   This research ultimately aimed at producing citrus stocks through in-vitro culture, and free of CVPD.  Citrus seeds were used as explant and cultured on MS and WPM in-vitro media, supplemented with malt extract. Citrus plantlets were tested for the presence of  CVPD DNA using CVPD specific primers in PCR reactions.   Research results showed that MS basal medium supplemented with malt extract was quite good for in- vitro production of citrus plantlets. Plantlets were negative for CVPD infection based on PCR tests.  CVPD-free seedlings (Citrus reticulata) have been grafted with citrus rootstock (Citrus sinensis).  Grafted citrus seedlings were also proven to be negative for CVPD infection based on similar PCR tests.  Citrus seedlings/stocks produced by in-vitro culture, and free of CVPD, are now available for further growth. Keywords: Citrus Vein Phloem Degeneration (CVPD), in-vitro culture, PCR</dc:description>
	<dc:publisher xml:lang="en-US">Fakultas Pertanian Universitas Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US">Fakultas Pertanian Universitas Halu Oleo</dc:contributor>
	<dc:date>2023-06-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/agroteknos/article/view/2249</dc:identifier>
	<dc:identifier>10.56189/ja.v1i3.2249</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Agroteknos; Vol 1, No 3 (2011)</dc:source>
	<dc:source>2527-4074</dc:source>
	<dc:source>10.56189/ja.v1i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/agroteknos/article/view/2249/1615</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Jurnal Agroteknos</dc:rights>
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