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EFEKTIVITAS LIMBAH CAIR PERTANIAN SEBAGAI MEDIA PERBANYAKAN DAN FORMULASI Bacillus subtilis SEBAGAI AGENS HAYATI PATOGEN TANAMAN

Jurnal Agroteknos · Vol. 3 No. 3 (2023)

DOI
10.56189/ja.v3i3.2323
ISSN
2527-4074
Published
2023-06-09
Publisher
Fakultas Pertanian Universitas Halu Oleo
Language
eng

Abstract

This study aimed to find the best medium for formulation and storage of B. subtilis. The study consisted of two phases: (1) Selection of agricultural wastes as a propagation medium for Bacillus subtilis, (2) test for the stability of Bacillus subtilis in material formulation and its inhibition activity against Rhizoctonia solani. The second phase was conducted based on completely randomized design, consisting of five treatments, namely: 100 % medium synthetic, 100% coconut water, 75% coconut water + 25 % synthetic medium, 50% coconut water + 50% synthetic medium and 25% coconut water + 75% synthetic medium. Each treatment was repeated three times, so that there were 15 experimental units. B. subtilis ST21e isolate was formulated in liquid medium according to treatment and kept in plastic container at room temperature for 8 weeks to count the number of colonies and inhibition activity every 2 weeks. The results showed that the agricultural wastes (coconut water, tofu water and molasses) can be used as a media for B. subtilis ST21e propogation in different cell growth pattern. B. subtilis propogation in medium coconut water + 10% TSB had the best growth pattern compared to the other media. On the other hand, medium containing 25% coconut water + 75% synthetic medium was the best combination for storage medium of B. subtilis ST21e. Key words: biological agents, Bacillus subtilis, agricultural waste

Collaboration network of the authors

40 authors · 209 co-authorship links · showing the 40 most connected

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  • Universitas Halu Oleo
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
TERRY PAKKIUniversitas Halu Oleo9
SRI WAHYUNIAffiliation unknown7
Asmar HasanUniversitas Halu Oleo7
Muhammad BotekUniversitas Halu Oleo6
MUHAMMAD TAUFIKAffiliation unknown6
Vit Neru SatrahUniversitas Halu Oleo6
Asniah AsniahUniversitas Halu Oleo6
Gusti Ayu Kade SutariatiUniversitas Halu Oleo5
MARIADI MARIADIUniversitas Halu Oleo5
AGUNG YUSWANAUniversitas Halu Oleo5
Abdul M. BalubiAffiliation unknown4
Irwan J. EffendyAffiliation unknown4
La Ode Santiaji BandeUniversitas Halu Oleo4
HS GusnawatyAffiliation unknown4
SYAIR SYAIRUniversitas Halu Oleo4
Waode Siti Anima HiseinUniversitas Halu Oleo3
NUR ISNAINI ULFAUniversitas Halu Oleo3
Teguh WijayantoAffiliation unknown3
Agus KurniaUniversitas Halu Oleo3
Ruslaini RuslainiAffiliation unknown3
Rahayu MallarangengUniversitas Halu Oleo3
La Ode Santiaji BandeAffiliation unknown3
Eka Lestari AriyantiAffiliation unknown3
Ma’ruf KasimUniversitas Halu Oleo2
Gusnawaty HSUniversitas Halu Oleo2
Aryuni Salpiana JabarUniversitas Halu Oleo1
Sarmadan SarmadanAffiliation unknown1
Agustono SlametUniversitas Halu Oleo1
LA ODE WALIUniversitas Halu Oleo1
Halim HalimUniversitas Halu Oleo1
Asdianti AsdiantiUniversitas Halu Oleo1
Hasriani HasrianiAffiliation unknown1
NURIADI NURIADIAffiliation unknown1
WAODE SITI ANIMA HISEINAffiliation unknown1
MUHAMMAD TAUFIKUniversitas Halu Oleo1
NUR INDAH SARIUniversitas Halu Oleo1
Gusnawaty H.SUniversitas Halu Oleo1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://ojs.uho.ac.id/index.php/agroteknos/oai, first harvested 31 Okt 2026, last seen 1 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">EFEKTIVITAS LIMBAH CAIR PERTANIAN SEBAGAI MEDIA PERBANYAKAN DAN FORMULASI  Bacillus subtilis SEBAGAI AGENS HAYATI PATOGEN TANAMAN</dc:title>
	<dc:creator>Khaeruni, Andi</dc:creator>
	<dc:creator>Asrianti, Asrianti</dc:creator>
	<dc:creator>Rahman, Abdul</dc:creator>
	<dc:description xml:lang="en-US">This study aimed to find the best medium for formulation and storage of B. subtilis. The study consisted of two phases: (1) Selection of agricultural wastes as a propagation medium for Bacillus subtilis, (2) test for the stability of Bacillus subtilis in material formulation and  its  inhibition  activity  against Rhizoctonia solani.  The  second  phase  was conducted based on completely randomized design, consisting of five treatments, namely: 100  %  medium  synthetic,  100%  coconut  water,  75%  coconut  water  +  25  %  synthetic medium, 50% coconut water + 50% synthetic medium and 25% coconut water + 75% synthetic medium. Each treatment was repeated three times, so that there were 15 experimental units. B. subtilis ST21e isolate was formulated in liquid medium according to treatment and kept in plastic container at room temperature for 8 weeks to count the number of colonies and inhibition activity every 2 weeks. The results showed that the agricultural wastes (coconut water, tofu water and molasses) can be used as a media for B. subtilis  ST21e  propogation  in  different  cell  growth  pattern.  B.  subtilis  propogation  in medium  coconut  water  +  10% TSB  had the  best growth pattern compared to the  other media. On the other hand, medium containing 25% coconut water + 75% synthetic medium was the best combination for storage medium of  B. subtilis ST21e. Key words: biological agents, Bacillus subtilis, agricultural waste</dc:description>
	<dc:publisher xml:lang="en-US">Fakultas Pertanian Universitas Halu Oleo</dc:publisher>
	<dc:contributor xml:lang="en-US">Fakultas Pertanian Universitas Halu Oleo</dc:contributor>
	<dc:date>2023-06-09</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://ojs.uho.ac.id/index.php/agroteknos/article/view/2323</dc:identifier>
	<dc:identifier>10.56189/ja.v3i3.2323</dc:identifier>
	<dc:source xml:lang="en-US">Jurnal Agroteknos; Vol 3, No 3 (2013)</dc:source>
	<dc:source>2527-4074</dc:source>
	<dc:source>10.56189/ja.v3i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://ojs.uho.ac.id/index.php/agroteknos/article/view/2323/1687</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 Jurnal Agroteknos</dc:rights>
</oai_dc:dc>