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Effect of FSH and PMSG Additions on Maturation and Fertilization Rates of Bali Cattle Oocytes

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 2 No. 2 (2021) · pp. 83–90

DOI
10.20956/hajas.v2i2.13735
ISSN
2621-9190, 2621-9182
Published
2021-06-07
Publisher
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

The process of in vitro oocyte maturation requires stimulation of gonadotropin hormones, as well as in vivo. There are two gonadotropin hormones that are available in the market and can be used in the process, Follicle Stimulating Hormone (FSH) and Pregnant Mare Serum Gonadotropin (PMSG). This study aimed to determine the effect of the addition of FSH and PMSG hormones in the maturation medium on the in vitro maturation and fertilization rates of Bali cattle oocytes. This study had two treatments, the addition of FSH and PMSG, each of which was carried out 4 times. Oocytes matured in basic medium, M-199 plus Bovine Serum Albumin (BSA) 10 IU/ml, and 50 µg/ml Gentamycin. Furthermore, the matured oocytes fertilized in 80 μl drop of sperms. The data were analyzed by Chi Square test to determine the differences in the level of maturation and fertilization of Bali cattle oocytes in vitro. The results of this study indicated that there was no significant difference (P>0.05) between the addition of FSH and PMSG hormones in the maturation medium to the nuclear maturation rate (81.4 ± 2.2 vs 81.2 ± 2.3), and the fertilization rate (42.2 ± 4.3 vs 24.6 ± 2.7). It can be concluded that in vitro maturation and fertilization rate of Bali cattle oocytes with the addition of FSH and PMSG hormones to the maturation medium are similar, but showed a tendency for higher fertilization rate in the medium supplemented with FSH.

Collaboration network of the authors

31 authors · 78 co-authorship links

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  • Universitas Halu Oleo
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Sudirman BacoAffiliation unknown5
Mutmainna MutmainnaAffiliation unknown4
Abdul Latief TolengAffiliation unknown3
Sitti FaridaAffiliation unknown3
Musrayani UsmanAffiliation unknown2
Kirana Dara Dinanti AdiputraAffiliation unknown2
Sukandi SukandiAffiliation unknown2
Siti FaridaAffiliation unknown2
Sulfikar SalluAffiliation unknown1
Ayyub AyyubAffiliation unknown1
Andi Nurul MukhlisahAffiliation unknown1
Tamrin TamrinUniversitas Halu Oleo1
LA KARIMUNAUniversitas Halu Oleo1
Akmal Achmad SulawantaAffiliation unknown1
Tahir KasnawiAffiliation unknown1
Hariashari RahimAffiliation unknown1
Sakaria SakariaAffiliation unknown1
Atma RasAffiliation unknown1
Muhammad Iqbal LatiefAffiliation unknown1
Andi Amirah Humairah YakubAffiliation unknown1
Nurul AminAffiliation unknown1
Wempie PakidingAffiliation unknown1
Ambo AkoAffiliation unknown1
Renny Fatmyah UtamyAffiliation unknown1
Jamila MustabiAffiliation unknown1
Utlul Ilma NaviaAffiliation unknown1
Andi Arif RahmanAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, first harvested 2 Nov 2026, last seen 2 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Effect of FSH and PMSG Additions on Maturation and Fertilization Rates of Bali Cattle Oocytes</dc:title>
	<dc:creator xml:lang="en">Hasbi, Hasbi</dc:creator>
	<dc:creator xml:lang="en">Sonjaya, Herry </dc:creator>
	<dc:creator xml:lang="en">Pawarekki, Andi Arya </dc:creator>
	<dc:creator xml:lang="en">Gustina, Sri</dc:creator>
	<dc:description xml:lang="en">The process of in vitro oocyte maturation requires stimulation of gonadotropin hormones, as well as in vivo. There are two gonadotropin hormones that are available in the market and can be used in the process, Follicle Stimulating Hormone (FSH) and Pregnant Mare Serum Gonadotropin (PMSG). This study aimed to determine the effect of the addition of FSH and PMSG hormones in the maturation medium on the in vitro maturation and fertilization rates of Bali cattle oocytes. This study had two treatments, the addition of FSH and PMSG, each of which was carried out 4 times. Oocytes matured in basic medium, M-199 plus Bovine Serum Albumin (BSA) 10 IU/ml, and 50 µg/ml Gentamycin. Furthermore, the matured oocytes fertilized in 80 μl drop of sperms. The data were analyzed by Chi Square test to determine the differences in the level of maturation and fertilization of Bali cattle oocytes in vitro. The results of this study indicated that there was no significant difference (P&amp;gt;0.05) between the addition of FSH and PMSG hormones in the maturation medium to the nuclear maturation rate (81.4 ± 2.2 vs 81.2 ± 2.3), and the fertilization rate (42.2 ± 4.3 vs 24.6 ± 2.7). It can be concluded that in vitro maturation and fertilization rate of Bali cattle oocytes with the addition of FSH and PMSG hormones to the maturation medium are similar, but showed a tendency for higher fertilization rate in the medium supplemented with FSH.</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2021-06-07</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/13735</dc:identifier>
	<dc:identifier>10.20956/hajas.v2i2.13735</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 2 No. 2 (2020); 83-90</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/13735/6974</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>