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Progressive Motility, DNA Fragmentation, Intact Plasma Membrane, and Acrosome Status of Frozen Semen Bali and Simmental Bulls

Hasanuddin Journal of Animal Science (HAJAS) · Vol. 4 No. 2 (2023) · pp. 109–118

DOI
10.20956/hajas.v4i2.23351
ISSN
2621-9190, 2621-9182
Published
2023-01-22
Publisher
Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia
Language
en
License
creativecommons.org/licenses/by-nc/4.0

Abstract

In increasing the population of bulls in Indonesia, it is necessary to utilize Artificial Insemination (AI) technology. AI reproductive technology aims to increase reproductive efficiency and use superior semen in livestock and can prevent the spread of reproductive diseases. Frozen semen is one of the factors that influence the success of AI. Thus, the quality of frozen semen must be maintained so fertility remains good. This study aimed to determine the quality of frozen semen of Bali and Simmental bulls at the Artificial Insemination Center, South Sulawesi. This study used frozen semen from two Bali and Simmental bulls. Parameters observed were progressive motility, deoxyribonucleic acid fragmentation (DNA), acrosome status, and intact plasma membrane. The progressive motility was tested using Computer-assisted sperm analysis (CASA Sperm Vision Minitube), the DNA fragmentation using acridine orange dye, the acrosome status using peanut agglutinin, and the intact plasma membrane staining using HOS solution. This study gives a result that the progressive motility, DNA fragmentation, and acrosome status of spermatozoa in frozen semen of Bali and Simmental bulls were not significantly different (P>0.05). In contrast, the whole plasma membrane of spermatozoa in Bali bulls' frozen semen was significantly higher (P<0.05) than in Simmental bulls. The results indicate that based on the parameters of progressive motility, DNA fragmentation, and acrosome status of spermatozoa, both bull breeds had the same quality of frozen semen. Meanwhile, according to the spermatozoa intact plasma membrane, the frozen semen of Bali bulls had better quality than that of Simmental bulls. Keywords: Artificial Insemination, frozen semen quality, Bali and Simmental bulls

Collaboration network of the authors

29 authors · 75 co-authorship links

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  • Universitas Halu Oleo
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Sri GustinaAffiliation unknown4
Sudirman BacoAffiliation unknown4
Mutmainna MutmainnaAffiliation unknown3
Musrayani UsmanAffiliation unknown2
Abdul Latief TolengAffiliation unknown2
Sitti FaridaAffiliation unknown2
Andi Arya PawarekkiAffiliation unknown2
Sulfikar SalluAffiliation unknown1
Tamrin TamrinUniversitas Halu Oleo1
LA KARIMUNAUniversitas Halu Oleo1
Akmal Achmad SulawantaAffiliation unknown1
Tahir KasnawiAffiliation unknown1
Hariashari RahimAffiliation unknown1
Sakaria SakariaAffiliation unknown1
Atma RasAffiliation unknown1
Muhammad Iqbal LatiefAffiliation unknown1
Andi Amirah Humairah YakubAffiliation unknown1
Nurul AminAffiliation unknown1
Wempie PakidingAffiliation unknown1
Ambo AkoAffiliation unknown1
Renny Fatmyah UtamyAffiliation unknown1
Jamila MustabiAffiliation unknown1
Utlul Ilma NaviaAffiliation unknown1
Andi Arif RahmanAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://journal.unhas.ac.id/index.php/hajas/oai, first harvested 2 Nov 2026, last seen 2 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Progressive Motility, DNA Fragmentation, Intact Plasma Membrane, and Acrosome Status of Frozen Semen Bali and Simmental Bulls</dc:title>
	<dc:creator xml:lang="en">Adiputra, Kirana Dara Dinanti</dc:creator>
	<dc:creator xml:lang="en">Sukandi, Sukandi </dc:creator>
	<dc:creator xml:lang="en">Farida, Siti</dc:creator>
	<dc:creator xml:lang="en">Sonjaya, Herry</dc:creator>
	<dc:creator xml:lang="en">Hasbi, Hasbi</dc:creator>
	<dc:description xml:lang="en">In increasing the population of bulls in Indonesia, it is necessary to utilize Artificial Insemination (AI) technology. AI reproductive technology aims to increase reproductive efficiency and use superior semen in livestock and can prevent the spread of reproductive diseases. Frozen semen is one of the factors that influence the success of AI. Thus, the quality of frozen semen must be maintained so fertility remains good. This study aimed to determine the quality of frozen semen of Bali and Simmental bulls at the Artificial Insemination Center, South Sulawesi. This study used frozen semen from two Bali and Simmental bulls. Parameters observed were progressive motility, deoxyribonucleic acid fragmentation (DNA), acrosome status, and intact plasma membrane. The progressive motility was tested using Computer-assisted sperm analysis (CASA Sperm Vision Minitube), the DNA fragmentation using acridine orange dye, the acrosome status using peanut agglutinin, and the intact plasma membrane staining using HOS solution. This study gives a result that the progressive motility, DNA fragmentation, and acrosome status of spermatozoa in frozen semen of Bali and Simmental bulls were not significantly different (P&amp;gt;0.05). In contrast, the whole plasma membrane of spermatozoa in Bali bulls' frozen semen was significantly higher (P&amp;lt;0.05) than in Simmental bulls. The results indicate that based on the parameters of progressive motility, DNA fragmentation, and acrosome status of spermatozoa, both bull breeds had the same quality of frozen semen. Meanwhile, according to the spermatozoa intact plasma membrane, the frozen semen of Bali bulls had better quality than that of Simmental bulls.
Keywords: Artificial Insemination, frozen semen quality, Bali and Simmental bulls</dc:description>
	<dc:publisher xml:lang="en">Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia</dc:publisher>
	<dc:date>2023-01-22</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unhas.ac.id/index.php/hajas/article/view/23351</dc:identifier>
	<dc:identifier>10.20956/hajas.v4i2.23351</dc:identifier>
	<dc:source xml:lang="en">Hasanuddin Journal of Animal Science (HAJAS); Vol. 4 No. 2 (2022); 109-118</dc:source>
	<dc:source>2621-9190</dc:source>
	<dc:source>2621-9182</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://journal.unhas.ac.id/index.php/hajas/article/view/23351/9237</dc:relation>
	<dc:rights xml:lang="en">http://creativecommons.org/licenses/by-nc/4.0</dc:rights>
</oai_dc:dc>