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ISOLATION OF LIMONOIDS FROM CLAUSENA EXCAVATA BURM.F AND THEIR ANTIBACTERIAL ACTIVITY AGAINST Enterococcus faecalis

Jurnal Ilmiah Ilmu Terapan Universitas Jambi · Vol. 9 No. 1 (2025) · pp. 216–226

DOI
10.22437/jiituj.v9i1.38597
ISSN
2580-2259, 2580-2240
Published
2025-02-17
Publisher
LPPM Universitas Jambi
Language
en
License
creativecommons.org/licenses/by/4.0

Abstract

This study aims to isolate and characterize limonoid compounds from Clausena excavata Burm.F and evaluate their antibacterial potential against Enterococcus faecalis, a pathogen associated with antibiotic resistance in healthcare settings. Plant samples were collected from natural habitats in Southeast Asia and identified by botanists for authenticity. The leaves, stems, and fruits were dried, ground into fine powder, and subjected to ethanol/methanol maceration, followed by liquid-liquid fractionation. Column chromatography and Thin Layer Chromatography (TLC) were used for initial compound separation, while High-Performance Liquid Chromatography (HPLC) was employed for further purification. Structural characterization was conducted using Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS), and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial activity of the isolated limonoids was tested against E. faecalis using the disk diffusion method, with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) determined through broth microdilution assays. The results indicate that limonoid compounds from Clausena excavata exhibit moderate antibacterial activity, producing inhibition zones smaller than standard antibiotics but still demonstrating potential for bacterial growth suppression. This study highlights Clausena excavata as a promising natural source of antibacterial agents, contributing to alternative solutions against antibiotic-resistant bacteria. The findings support further exploration of limonoids as lead compounds for novel antimicrobial drugs, promoting the use of natural products in combating antibiotic resistance and advancing phytopharmaceutical applications in healthcare.

Keywords

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8 authors · 24 co-authorship links

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  • Universitas Jambi
  • Affiliation unknown
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Co-authorOrganisationJoint articles
Husni SabilUniversitas Jambi3
Novferma NovfermaAffiliation unknown3
Syaiful SyaifulAffiliation unknown2
Mujahidawati MujahidawatiAffiliation unknown2
M Afifuddin Khotibul UmamAffiliation unknown2
Nizlel HudaUniversitas Jambi1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://online-journal.unja.ac.id/index.php/JIITUJ/oai, first harvested 5 Nov 2026, last seen 5 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">ISOLATION OF LIMONOIDS FROM CLAUSENA EXCAVATA BURM.F AND THEIR ANTIBACTERIAL ACTIVITY AGAINST Enterococcus faecalis</dc:title>
	<dc:creator xml:lang="en">Harizon, Harizon</dc:creator>
	<dc:creator xml:lang="en">Romundza, Febbry</dc:creator>
	<dc:subject xml:lang="en">Antibacterial Activity</dc:subject>
	<dc:subject xml:lang="en">Clausena Excavata</dc:subject>
	<dc:subject xml:lang="en">Enterococcus Faecalis</dc:subject>
	<dc:subject xml:lang="en">Extraction Limonoids</dc:subject>
	<dc:subject xml:lang="en">Spectroscopy</dc:subject>
	<dc:description xml:lang="en">This study aims to isolate and characterize limonoid compounds from Clausena excavata Burm.F and evaluate their antibacterial potential against Enterococcus faecalis, a pathogen associated with antibiotic resistance in healthcare settings. Plant samples were collected from natural habitats in Southeast Asia and identified by botanists for authenticity. The leaves, stems, and fruits were dried, ground into fine powder, and subjected to ethanol/methanol maceration, followed by liquid-liquid fractionation. Column chromatography and Thin Layer Chromatography (TLC) were used for initial compound separation, while High-Performance Liquid Chromatography (HPLC) was employed for further purification. Structural characterization was conducted using Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS), and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial activity of the isolated limonoids was tested against E. faecalis using the disk diffusion method, with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) determined through broth microdilution assays. The results indicate that limonoid compounds from Clausena excavata exhibit moderate antibacterial activity, producing inhibition zones smaller than standard antibiotics but still demonstrating potential for bacterial growth suppression. This study highlights Clausena excavata as a promising natural source of antibacterial agents, contributing to alternative solutions against antibiotic-resistant bacteria. The findings support further exploration of limonoids as lead compounds for novel antimicrobial drugs, promoting the use of natural products in combating antibiotic resistance and advancing phytopharmaceutical applications in healthcare.</dc:description>
	<dc:publisher xml:lang="en">LPPM Universitas Jambi</dc:publisher>
	<dc:date>2025-02-17</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://online-journal.unja.ac.id/JIITUJ/article/view/38597</dc:identifier>
	<dc:identifier>10.22437/jiituj.v9i1.38597</dc:identifier>
	<dc:source xml:lang="en">Jurnal Ilmiah Ilmu Terapan Universitas Jambi; Vol. 9 No. 1 (2025): Volume 9, Nomor 1, March 2025; 216-226</dc:source>
	<dc:source>2580-2259</dc:source>
	<dc:source>2580-2240</dc:source>
	<dc:language>en</dc:language>
	<dc:relation>https://online-journal.unja.ac.id/JIITUJ/article/view/38597/20150</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Harizon Harizon, Febbry Romundza</dc:rights>
	<dc:rights xml:lang="en">https://creativecommons.org/licenses/by/4.0</dc:rights>
</oai_dc:dc>