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Pengaruh Variasi Metode Ekstraksi Metabolit Sekunder Bakteri Endofit Daun Rambutan (Nephelium lappaceum L.) terhadap Pertumbuhan Escherichia coli dan Bacillus subtilis

Life Science · Vol. 13 No. 2 (2024) · hlm. 144–160

DOI
10.15294/unnesjlifesci.v13.i2.2327
ISSN
2528-5009, 2252-6277
Terbit
2024-11-07
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Rambutan (Nephelium lappaceum L.) is one of the many tropical plants found in Indonesia. The leaves of the rambutan plant are known to be used as medicine, so they have the potential to be a source of endophytic bacteria that can produce beneficial compound. This study aims to isolate, identify of endophytic bacteria from rambutan leaves, and test their activity as an antibacterial with two different secondary metabolite extraction methods, namely maceration with 70% methanol and without maceration. This study used experimental research with a Completely Ramdomized Design (CRD). Isolation of endophytic bacteria from rambutan leaves produced two isolates (D1 and D2). The results of identification through macroscopic, microscopic, and biochemical test showed that two pure isolates had similarities to genus Bacillus sp. Antibacterial activity testing was carried out using well diffusion method. Based on the results of antibacterial test showed that extraction method with 70% methanol produced an extract that was more effective as an antibacterial with average diameter of clear zone formed for Escherichia coli bacteria were 13.55 mm (Isolate D1) and 12.13 mm (Isolate D2) and that of Bacillus subtilis bacteria were 16.27 mm (Isolate D1) and 19.69 mm (Isolate D2). This finding contributes to the fact that the type of secondary metabolite extraction method of rambutan leaf endophytic bacteria influences the sensivity as an antibacterial.

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  • State University of Semarang
  • Diponegoro University
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Rekan penulisOrganisasiArtikel bersama
Siti Harnina BintariAfiliasi tidak diketahui26
Talitha WidiatningrumAfiliasi tidak diketahui7
Dyah Rini IndriyantiState University of Semarang4
Ari YuniastutiState University of Semarang4
Priyantini WidiyaningrumAfiliasi tidak diketahui2
Maria Ayu PuspitaAfiliasi tidak diketahui2
Nunung Eni ElawatiAfiliasi tidak diketahui2
Andhina Putri HeriyantiDiponegoro University2
Arif WidiyatmokoState University of Semarang2
Fidia FibrianaState University of Semarang2
Mutiara Bintang RamadaniAfiliasi tidak diketahui2
Astrid FarmayatiAfiliasi tidak diketahui2
Ghani WalandipaAfiliasi tidak diketahui2
Lusi Maswahyu AndriyaniAfiliasi tidak diketahui2
SunyotoAfiliasi tidak diketahui2
Nur Rahayu UtamiAfiliasi tidak diketahui2
Sigit SaptonoState University of Semarang2
Sri SukaesihState University of Semarang2
Ernallah YatiAfiliasi tidak diketahui1
Aura Tirsa MelindaAfiliasi tidak diketahui1
Rahimma Artantia AnandaAfiliasi tidak diketahui1
Orchida Puspa JelitaAfiliasi tidak diketahui1
Delia Alifia FarrezaAfiliasi tidak diketahui1
Supriyadi SupriyadiAfiliasi tidak diketahui1
Kusmuriyanto KusmuriyantoAfiliasi tidak diketahui1
Mochamad Nur SholikhinAfiliasi tidak diketahui1
Nishfa MufatihahAfiliasi tidak diketahui1
Niken SubektiState University of Semarang1
Ning SetiatiAfiliasi tidak diketahui1
Ely RudyatmiAfiliasi tidak diketahui1
Sriyadi SriyadiAfiliasi tidak diketahui1
Rusyida AuliyaAfiliasi tidak diketahui1
Naeli Tamin QonitaAfiliasi tidak diketahui1
Previanda ArditamaAfiliasi tidak diketahui1
Nana Kariada Tri MartutiAfiliasi tidak diketahui1
Elfrida Theresia SiburianAfiliasi tidak diketahui1

Afiliasi berasal dari OpenAlex; metadata OAI-PMH jurnal tidak memuatnya. Penulis tanpa ID OpenAlex dicocokkan berdasarkan nama, sehingga dua orang bisa tergabung atau satu orang bisa terpisah.

Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/UnnesJLifeSci/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 5 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Pengaruh Variasi Metode Ekstraksi Metabolit Sekunder Bakteri Endofit Daun Rambutan (Nephelium lappaceum L.) terhadap Pertumbuhan Escherichia coli dan Bacillus subtilis</dc:title>
	<dc:creator xml:lang="en">Hasyim Abdurrasyid</dc:creator>
	<dc:creator xml:lang="en">Ibnul Mubarok</dc:creator>
	<dc:creator xml:lang="en">Dewi Mustikaningtyas</dc:creator>
	<dc:creator xml:lang="en">Pramesti Dewi</dc:creator>
	<dc:subject xml:lang="en">Antibacterial</dc:subject>
	<dc:subject xml:lang="en">Endophytic bacteria</dc:subject>
	<dc:subject xml:lang="en">Maceration</dc:subject>
	<dc:subject xml:lang="en">Methanol</dc:subject>
	<dc:subject xml:lang="en">Rambutan leaf</dc:subject>
	<dc:description xml:lang="en">Rambutan (Nephelium lappaceum L.) is one of the many tropical plants found in Indonesia. The leaves of the rambutan plant are known to be used as medicine, so they have the potential to be a source of endophytic bacteria that can produce beneficial compound. This study aims to isolate, identify of endophytic bacteria from rambutan leaves, and test their activity as an antibacterial with two different secondary metabolite extraction methods, namely maceration with 70% methanol and without maceration. This study used experimental research with a Completely Ramdomized Design (CRD). Isolation of endophytic bacteria from rambutan leaves produced two isolates (D1 and D2). The results of identification through macroscopic, microscopic, and biochemical test showed that two pure isolates had similarities to genus Bacillus sp. Antibacterial activity testing was carried out using well diffusion method. Based on the results of antibacterial test showed that extraction method with 70% methanol produced an extract that was more effective as an antibacterial with average diameter of clear zone formed for Escherichia coli bacteria were 13.55 mm (Isolate D1) and 12.13 mm (Isolate D2) and that of Bacillus subtilis bacteria were 16.27 mm (Isolate D1) and 19.69 mm (Isolate D2). This finding contributes to the fact that the type of secondary metabolite extraction method of rambutan leaf endophytic bacteria influences the sensivity as an antibacterial.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2024-11-07</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/UnnesJLifeSci/article/view/2327</dc:identifier>
	<dc:identifier>10.15294/unnesjlifesci.v13.i2.2327</dc:identifier>
	<dc:source xml:lang="en">Life Science; Vol. 13 No. 2 (2024): November 2024; 144-160</dc:source>
	<dc:source>2528-5009</dc:source>
	<dc:source>2252-6277</dc:source>
	<dc:source>10.15294/lifesci</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/UnnesJLifeSci/article/view/2327/1899</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2024 Life Science</dc:rights>
</oai_dc:dc>