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Numeric–Phenetic Study of Lablab purpureus from East Java Based on Morphology Character and DNA Fingerprinting

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 1 (2025) · hlm. 9–18

DOI
10.15294/biosaintifika.v17i1.4604
ISSN
2338-7610, 2085-191X
Terbit
2025-04-20
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

This research aims to conduct a numeric–phenetic study of Lablab purpureus (L) Sweet based on morphology character and DNA fingerprinting with cophenetic–correlation algorithm confirmation. DNA genome extraction is based on the Blood Animal Plant DNA Preparation Kit. Ten RAPD primers were chosen for the amplification DNA genome, including OPA-6, OPA-8, OPA-10, OPA-20, OPC-19, OPD-8, OPD-12, OPE-8, OPE-15, and OPE-16. Cophenetic correlation is used to analyze three clustering algorithms (Nearest Neighbor, UPGMA, Farthest Neighbor) and five similarity indexes (Simple Matching, Jaccard, Nei & Li, Sorensen, Yule). Lablab purpureus (L) Sweet samples have shown morphology variation, mostly on flowers, pods, and seeds. DNA fingerprinting reveals a variety of band numbers ranging from 300BP to 1000BP. RAPD analysis revealed a total of 87 bands, including 18 polymorphic bands, with an average percentage polymorphism of 31.15%. The UPGMA technique was developed as a result of cophenetic-correlation analysis, and the Jaccard similarity index is the optimal combination for dendrogram generation. The topology of a morphology character dendrogram differs slightly from that of a DNA fingerprinting dendrogram. It concluded that the UPGMA algorithm and Jaccard similarity index is the optimum combination to analyze the diversity of L. purpureus (L) Sweet accession from East Java, resulting in the accession of this species having high diversity. This research can give a novel approaching method in the taxonomy field, which is combining morphological and molecular data.

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  • Universitas Muhammadiyah Malang
  • University of Brawijaya
  • University of Mataram
  • Universitas PGRI Madiun
  • Universitas Bhakti Kencana
  • University of Veterinary Medicine Vienna
  • State University of Malang
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Rekan penulisOrganisasiArtikel bersama
Muhammad Arief RizkaAfiliasi tidak diketahui3
Hadi Gunawan SaktiUniversity of Mataram3
Tutut Indria PermanaUniversitas Muhammadiyah Malang3
Marheny LukitasariUniversitas PGRI Madiun3
Feri Eko HermantoUniversity of Brawijaya2
I Gusti Ngurah Agung WiwekanandaUniversity of Brawijaya2
K.l U. MaududyAfiliasi tidak diketahui1
Gito HadiprayitnoUniversity of Mataram1
Frendi GunawanAfiliasi tidak diketahui1
I Gusti Agung Ayu Hari TriandiniUniversitas Bhakti Kencana1
Moh. Mirza NuryadyUniversity of Veterinary Medicine Vienna1
Kiky Martha AriesakaState University of Malang1
Ieke Wulan AyuUniversitas Samawa1
SoemarnoUniversity of Brawijaya1
Jeffry HandhikaUniversitas PGRI Madiun1
Rusdi HasanUniversitas Muhammadiyah Bengkulu1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en">Numeric–Phenetic Study of Lablab purpureus from East Java Based on Morphology Character and DNA Fingerprinting</dc:title>
	<dc:creator xml:lang="en">Purwanti, Elly</dc:creator>
	<dc:creator xml:lang="en">Wangiyana, I Gde Adi Suryawan</dc:creator>
	<dc:creator xml:lang="en">Prihanta, Wahyu</dc:creator>
	<dc:creator xml:lang="en">Sukri, Akhmad</dc:creator>
	<dc:subject xml:lang="en">DNA Fingerprinting</dc:subject>
	<dc:subject xml:lang="en">Morphology Character</dc:subject>
	<dc:subject xml:lang="en">Numeric-Phenetic</dc:subject>
	<dc:subject xml:lang="en">Lablab purpureus</dc:subject>
	<dc:description xml:lang="en">This research aims to conduct a numeric–phenetic study of Lablab purpureus (L) Sweet based on morphology character and DNA fingerprinting with cophenetic–correlation algorithm confirmation. DNA genome extraction is based on the Blood Animal Plant DNA Preparation Kit. Ten RAPD primers were chosen for the amplification DNA genome, including OPA-6, OPA-8, OPA-10, OPA-20, OPC-19, OPD-8, OPD-12, OPE-8, OPE-15, and OPE-16. Cophenetic correlation is used to analyze three clustering algorithms (Nearest Neighbor, UPGMA, Farthest Neighbor) and five similarity indexes (Simple Matching, Jaccard, Nei &amp;amp; Li, Sorensen, Yule). Lablab purpureus (L) Sweet samples have shown morphology variation, mostly on flowers, pods, and seeds. DNA fingerprinting reveals a variety of band numbers ranging from 300BP to 1000BP. RAPD analysis revealed a total of 87 bands, including 18 polymorphic bands, with an average percentage polymorphism of 31.15%. The UPGMA technique was developed as a result of cophenetic-correlation analysis, and the Jaccard similarity index is the optimal combination for dendrogram generation. The topology of a morphology character dendrogram differs slightly from that of a DNA fingerprinting dendrogram. It concluded that the UPGMA algorithm and Jaccard similarity index is the optimum combination to analyze the diversity of L. purpureus (L) Sweet accession from East Java, resulting in the accession of this species having high diversity. This research can give a novel approaching method in the taxonomy field, which is combining morphological and molecular data.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-04-20</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/4604</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i1.4604</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 1 (2025): April 2025; 9-18</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i1</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/4604/3755</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
</oai_dc:dc>