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Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia

Biosaintifika: Journal of Biology & Biology Education · Vol. 17 No. 3 (2025) · hlm. 382–394

DOI
10.15294/biosaintifika.v17i3.26663
ISSN
2338-7610, 2085-191X
Terbit
2025-11-18
Penerbit
Universitas Negeri Semarang
Bahasa
eng

Abstrak

Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.

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  • Universitas Negeri Surabaya
  • National Research and Innovation Agency
  • Universitas Nahdlatul Ulama Pasuruan
  • University of Malaya
  • Universitas Bhayangkara Surabaya
  • Poltekkes Kemenkes Banjarmasin
  • Universitas Jember
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Rekan penulisOrganisasiArtikel bersama
Haryono HaryonoUniversitas Bhayangkara Surabaya4
Nia KurniawanAfiliasi tidak diketahui4
Reni AmbarwatiAfiliasi tidak diketahui2
Abdul BasithAfiliasi tidak diketahui2
Sunu KuntjoroAfiliasi tidak diketahui2
Widowati BudijastutiAfiliasi tidak diketahui2
Winarsih WinarsihAfiliasi tidak diketahui2
Rodliyati AzrianingzihAfiliasi tidak diketahui2
Isnawati -Poltekkes Kemenkes Banjarmasin1
Guntur TrimulyonoAfiliasi tidak diketahui1
Mahanani Tri AsriAfiliasi tidak diketahui1
Pramudya Dwi Aristya PutraUniversitas Jember1
Ifatur RohmawatiAfiliasi tidak diketahui1
Gema WahyudewantoroNational Research and Innovation Agency1
Kunto WibowoIndonesian Institute of Sciences1
Ibrohim IbrohimState University of Malang1
Dwi ListyoriniAfiliasi tidak diketahui1

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Sumber metadata: OAI-PMH (metadata jurnal) https://journal.unnes.ac.id/journals/biosaintifika/oai, pertama di-harvest 5 Nov 2026, terakhir terlihat 8 Nov 2026.
Metadata asli (oai_dc) 
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	<dc:title xml:lang="en">Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia</dc:title>
	<dc:creator xml:lang="en">Panjaitan, Viona Angelina Erlan </dc:creator>
	<dc:creator xml:lang="en">Rangga, Adam Dwi </dc:creator>
	<dc:creator xml:lang="en">Sulung, Cahya Ajeng Valenta Tresna </dc:creator>
	<dc:creator xml:lang="en">Rahayu, Dwi Anggorowati</dc:creator>
	<dc:creator xml:lang="en">Khaleyla, Firas </dc:creator>
	<dc:creator xml:lang="en">Nugroho, Endik Deni </dc:creator>
	<dc:creator xml:lang="en">Rusdianto, Rusdianto </dc:creator>
	<dc:creator xml:lang="en">Mamat, Noorhidayah Binti </dc:creator>
	<dc:subject xml:lang="en">diagnostic characters</dc:subject>
	<dc:subject xml:lang="en">DNA barcodes</dc:subject>
	<dc:subject xml:lang="en">Genetic composition</dc:subject>
	<dc:subject xml:lang="en">Phenetic</dc:subject>
	<dc:description xml:lang="en">Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.</dc:description>
	<dc:publisher xml:lang="en">Universitas Negeri Semarang</dc:publisher>
	<dc:date>2025-11-18</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.unnes.ac.id/journals/biosaintifika/article/view/26663</dc:identifier>
	<dc:identifier>10.15294/biosaintifika.v17i3.26663</dc:identifier>
	<dc:source xml:lang="en">Biosaintifika: Journal of Biology &amp; Biology Education; Vol. 17 No. 3 (2025): November 2025; 382-394</dc:source>
	<dc:source>2338-7610</dc:source>
	<dc:source>2085-191X</dc:source>
	<dc:source>10.15294/biosaintifika.v17i3</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.unnes.ac.id/journals/biosaintifika/article/view/26663/7112</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2025 Biosaintifika: Journal of Biology &amp; Biology Education</dc:rights>
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