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Production, Characterization, and Molecular Phylogenetic Analysis of Phytase from Aspergillus niger Isolates of an Indonesia Origin

HAYATI Journal of Biosciences · Vol. 29 No. 4 (2022) · pp. 468–478

DOI
10.4308/hjb.29.4.468-478
ISSN
2086-4094, 1978-3019
Published
2022-04-06
Publisher
Bogor Agricultural University, Indonesia
Language
eng

Abstract

This research aimed at analyzing the phytase from fungal isolates SB1, SB2, BS, and WF produced in cornstarch with glucose medium (CS+Glu) as carbon sources and Potatoes dextrose broth (PDB). The activity of phytase from isolates SB1, SB2, BS, and WF produced in CS+Glu medium was 2.97 UmL-1, 2.87 UmL-1, 3.18 UmL-1, and 4.37 UmL-1, respectively, while the activity of phytases was 2.07 UmL-1, 2.17 UmL-1, 2.22 UmL-1, and 2.78 UmL-1 respectively in PDB medium. The optimal temperature of SB1 and WF phytase was 40°C, while SB2 and BS were 50°C and 60°C, respectively. The optimal pH of SB1 and WF phytase was 5.0, while SB2 and BS phytase were 6.0, and 4.0 respectively. 18S rRNA gene analysis revealed that SB1 was 99% identical to Aspergillus niger ANTS (KY825168.1), SB2, BS, and WF were 99% identical to A. niger Moriga leaf (MG889596.1). Multiple sequences and phylogenetic analysis of phytase gene showed that phyA_SB1 and phyA_SB2 were 98% homology with A. ficuum (AAB26466), 97% with A. niger (ADP05107) while phyA_WF was 99% with A. ochraceoroseus (PLB29348), 98% with A. niger (ADP05105). The deduced proteins contain conserved motifs RHGARYPTD at N-terminal while lacking HD motif at C-terminal. These phytases were in the same cluster with Aspergillus sp. phytase A indicating that they belong to Histidine Acid Phosphatases (HAP) family.

Collaboration network of the authors

40 authors · 107 co-authorship links · 15 across different organisations · showing the 40 most connected

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  • Sebelas Maret University
  • State University of Semarang
  • Universiti Brunei Darussalam
  • National University of Malaysia
  • Indonesian Institute of Sciences
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
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Co-authorOrganisationJoint articles
Suranto SurantoSebelas Maret University4
Muhammad AkhyarSebelas Maret University3
Ratna SetyaningsihAffiliation unknown3
Idam Ragil Widianto AtmojoSebelas Maret University2
Riezky Maya ProbosariSebelas Maret University2
Ashadi AshadiSebelas Maret University2
S. SutiknoState University of Semarang2
Sri WidoretnoSebelas Maret University2
Sri DwiastutiSebelas Maret University2
Siti Lusi Arum SariAffiliation unknown2
Prabang SetyonoSebelas Maret University2
Basori BasoriAffiliation unknown2
Wiranto WirantoAffiliation unknown2
Mohammad MasykuriSebelas Maret University2
Roy ArdiansyahAffiliation unknown1
Dwi Yuniasih SaputriAffiliation unknown1
Roslinawati Mohd RoslanUniversiti Brunei Darussalam1
Lilia HalimNational University of Malaysia1
Dini Yuli SaputriSebelas Maret University1
R. EtvianaSebelas Maret University1
Bertha WikaraSebelas Maret University1
S. SutarnoSebelas Maret University1
Baskoro Adi PrayitnoSebelas Maret University1
Afandi AfandiSebelas Maret University1
Nunuk SuryaniSebelas Maret University1
P. ParminState University of Semarang1
Fidia FibrianaState University of Semarang1
Annisa Nur KhasanahSebelas Maret University1
Ade Cyntia PritasariSebelas Maret University1
Parmin ParminState University of Semarang1
Sutarno SutarnoIndonesian Institute of Sciences1
Sigit PrastowoAffiliation unknown1
Shavya Sarah SavieraAffiliation unknown1
Rebecca VanessaAffiliation unknown1
Galih PambukoAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) http://journal.ipb.ac.id/index.php/hayati/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en">Production, Characterization, and Molecular Phylogenetic Analysis of Phytase from Aspergillus niger Isolates of an Indonesia Origin</dc:title>
	<dc:creator xml:lang="en">Mkumbe, Baraka Stewart</dc:creator>
	<dc:creator xml:lang="en">Sajidan, Sajidan</dc:creator>
	<dc:creator xml:lang="en">Pangastuti, Artini</dc:creator>
	<dc:creator xml:lang="en">Susilowati, Ari</dc:creator>
	<dc:creator xml:lang="en">Greiner, Ralf</dc:creator>
	<dc:description xml:lang="en">This research aimed at analyzing the phytase from fungal isolates SB1, SB2, BS, and WF produced in cornstarch with glucose medium (CS+Glu) as carbon sources and Potatoes dextrose broth (PDB). The activity of phytase from isolates SB1, SB2, BS, and WF produced in CS+Glu medium was 2.97 UmL-1, 2.87 UmL-1, 3.18 UmL-1, and 4.37 UmL-1, respectively, while the activity of phytases was 2.07 UmL-1, 2.17 UmL-1, 2.22 UmL-1, and 2.78 UmL-1 respectively in PDB medium. The optimal temperature of SB1 and WF phytase was 40°C, while SB2 and BS were 50°C and 60°C, respectively. The optimal pH of SB1 and WF phytase was 5.0, while SB2 and BS phytase were 6.0, and 4.0 respectively. 18S rRNA gene analysis revealed that SB1 was 99% identical to Aspergillus niger ANTS (KY825168.1), SB2, BS, and WF were 99% identical to A. niger Moriga leaf (MG889596.1). Multiple sequences and phylogenetic analysis of phytase gene showed that phyA_SB1 and phyA_SB2 were 98% homology with A. ficuum (AAB26466), 97% with A. niger (ADP05107) while phyA_WF was 99% with A. ochraceoroseus (PLB29348), 98% with A. niger (ADP05105). The deduced proteins contain conserved motifs RHGARYPTD at N-terminal while lacking HD motif at C-terminal. These phytases were in the same cluster with Aspergillus sp. phytase A indicating that they belong to Histidine Acid Phosphatases (HAP) family.</dc:description>
	<dc:publisher xml:lang="en">Bogor Agricultural University, Indonesia</dc:publisher>
	<dc:date>2022-04-06</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en">Peer-reviewed Article</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>http://journal.ipb.ac.id/hayati/article/view/40622</dc:identifier>
	<dc:identifier>10.4308/hjb.29.4.468-478</dc:identifier>
	<dc:source xml:lang="en">HAYATI Journal of Biosciences; Vol. 29 No. 4 (2022): July 2022; 468-478</dc:source>
	<dc:source>2086-4094</dc:source>
	<dc:source>1978-3019</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>http://journal.ipb.ac.id/hayati/article/view/40622/23267</dc:relation>
	<dc:rights xml:lang="en">Copyright (c) 2022 Baraka Stewart Mkumbe, Sajidan Sajidan, Artini Pangastuti, Ari Susilowati, Ralf Greiner</dc:rights>
</oai_dc:dc>