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Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

Indonesian Journal of Biotechnology · Vol. 10 No. 1 (2005)

DOI
10.22146/ijbiotech.7411
ISSN
2089-2241, 0853-8654
Published
2005-06-13
Publisher
Universitas Gadjah Mada
Language
eng

Abstract

Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the research was to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinant technology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and it was used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was then digesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1- encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein. Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed 100% homologous.

Collaboration network of the authors

40 authors · 103 co-authorship links · showing the 40 most connected

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  • Mulawarman University
  • Universitas Gadjah Mada
  • University of Brawijaya
  • Universitas Trisakti
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Endang SemiartiAffiliation unknown6
Aziz PurwantoroAffiliation unknown5
Rosita DewiMulawarman University3
Irawan Fajar KusumaAffiliation unknown3
Widya AsmaraUniversitas Gadjah Mada3
Didik Tulus SubektiAffiliation unknown3
Sheilla RachmaniaAffiliation unknown2
Sahrir SillehuAffiliation unknown2
Nintya SetiariAffiliation unknown2
Sri DarmawatiAffiliation unknown2
LANGKAH SEMBIRINGAffiliation unknown2
Exsyupransia MursyantiAffiliation unknown2
Yulia SariAffiliation unknown2
Soenarwan Hery PoerwantoAffiliation unknown2
Tsabita NafisaAffiliation unknown1
Ni Nyoman Ayu DewiAffiliation unknown1
S. SumartonoAffiliation unknown1
Dyah Ayu Oktavianie A PratamaAffiliation unknown1
Syaiful AnwarAffiliation unknown1
Masashi KawaichiAffiliation unknown1
Yoga PratamaUniversity of Brawijaya1
Subagus WahyuonoAffiliation unknown1
Tri R. NuringtyasAffiliation unknown1
G. GalihAffiliation unknown1
Ixora Sartika MercurianiAffiliation unknown1
Seonghoe JangAffiliation unknown1
Pudji AstutiUniversitas Trisakti1
Laurentius Hartanto NugrohoAffiliation unknown1
Retno AryaniAffiliation unknown1
Sri WahyuningsihAffiliation unknown1
Budi Setiadi DaryonoAffiliation unknown1
Muhammad Dylan LawrieAffiliation unknown1
Soenghoe JangAffiliation unknown1
Resy Metri BelizaniAffiliation unknown1
Loeki Enggar FitriAffiliation unknown1
Samsul ArifinAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate</dc:title>
	<dc:creator>Sulistyaningsih, Erma</dc:creator>
	<dc:creator>Moeljopawiro, Sukarti</dc:creator>
	<dc:creator>Subandono, Jarot</dc:creator>
	<dc:creator>Artama, Wayan T.</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the research was to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinant technology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and it was used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was then digesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1- encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein. Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed 100% homologous.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2005-06-13</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7411</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7411</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 10, No 1 (2005)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7411/5765</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>