Melola Discovery

← Melola Discovery

Journal SINTA 1

Cloning and Expression of ORF124 Koi Herpesvirus as a Vaccine

Indonesian Journal of Biotechnology · Vol. 17 No. 1 (2012)

DOI
10.22146/ijbiotech.7850
ISSN
2089-2241, 0853-8654
Published
2012-06-03
Publisher
Universitas Gadjah Mada
Language
eng

Abstract

Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3 (CyHV-3), Koi herpes-like virus,and carp interstitial nephritis gill necrosis virus (CNGV), caused signifi cant morbidity and mortality in koiand common carp (Cyprinus carpio). The case fatality rate of this disease is 80–100%. Glycoprotein has beenused for vaccine development as sub unit vaccine against viruses. The aim of this research was to clone andexpress membrane glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV gene wassuccessfully cloned into pBSKS and sequenced. Result showed that ORF124 KHV (isolate from Indonesia) had100 % similarity with Cyprinid herpesvirus 3 strain TUMST1 (from Japan), 99% similarity with Koi herpesvirus strain KHV-U (from USA) and Koi herpesvirus strain KHV-I (from Israel). Prediction analysis of T and B cellepitopes showed that ORF124 KHV protein had 14 and 11 T cell epitopes (IAd, Rothbard/Taylor pattern),and had 10 B cell epitopes, suggested that the protein can be used as a vaccine candidate. ORF124 gene hasbeen expressed in Escherichia coli under pET32-a(+)vector.

Collaboration network of the authors

40 authors · 101 co-authorship links · showing the 40 most connected

Hover or tap a name to highlight its co-authors. Double-click to open the author's page.

  • Universitas Gadjah Mada
  • Universitas Trisakti
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Rumiyati RumiyatiAffiliation unknown3
Firman AlamsyahAffiliation unknown3
Yekti Asih PurwestriAffiliation unknown3
Edy MeiyantoAffiliation unknown2
Muhammad Da’iAffiliation unknown2
Pudji AstutiUniversitas Trisakti2
LANGKAH SEMBIRINGAffiliation unknown2
Woro Anindito Sri TunjungAffiliation unknown2
Alfi Rizqi AmaliaAffiliation unknown2
Chio OkaAffiliation unknown2
Christina Retna HandayaniAffiliation unknown2
Norman Arie PrayogoAffiliation unknown1
Asrul Sahri SiregarAffiliation unknown1
Purnama SukardiAffiliation unknown1
Taufik Budi PramonoAffiliation unknown1
Rita MalizaAffiliation unknown1
Putra SantosoAffiliation unknown1
Bramadi AryaAffiliation unknown1
Alimuddin TofrizalAffiliation unknown1
Suwarno HadisusantoUniversitas Gadjah Mada1
HaryantiAffiliation unknown1
Sari Budi Moria SembiringAffiliation unknown1
Jhon Harianto HutapeaAffiliation unknown1
I Nyoman Adiasmara GiriAffiliation unknown1
Ketut MahardikaAffiliation unknown1
Umar Anggara JenieAffiliation unknown1
Supardjan AMAffiliation unknown1
Jaka WidadaUniversitas Gadjah Mada1
Yani Lestari NurainiAffiliation unknown1
Barinta WidaryantiAffiliation unknown1
Asmarani KusumawatiAffiliation unknown1
Penny Humaidah HamidAffiliation unknown1
Widya AsmaraUniversitas Gadjah Mada1
Sri DarmawatiAffiliation unknown1
Wayan T. ArtamaAffiliation unknown1
R. RumiyatiAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
<oai_dc:dc xmlns="http://www.openarchives.org/OAI/2.0/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/  http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
	<dc:title xml:lang="en-US">Cloning and Expression of ORF124 Koi Herpesvirus as a Vaccine</dc:title>
	<dc:creator>Murwantoko, M.</dc:creator>
	<dc:creator>Setyowati, Dewi Nur'aeni</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Kawaichi, Masashi</dc:creator>
	<dc:subject xml:lang="en-US"></dc:subject>
	<dc:description xml:lang="en-US">  Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3 (CyHV-3), Koi herpes-like virus,and carp interstitial nephritis gill necrosis virus (CNGV), caused signifi cant morbidity and mortality in koiand common carp (Cyprinus carpio). The case fatality rate of this disease is 80–100%. Glycoprotein has beenused for vaccine development as sub unit vaccine against viruses. The aim of this research was to clone andexpress membrane glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV gene wassuccessfully cloned into pBSKS and sequenced. Result showed that ORF124 KHV (isolate from Indonesia) had100 % similarity with Cyprinid herpesvirus 3 strain TUMST1 (from Japan), 99% similarity with Koi herpesvirus strain KHV-U (from USA) and Koi herpesvirus strain KHV-I (from Israel). Prediction analysis of T and B cellepitopes showed that ORF124 KHV protein had 14 and 11 T cell epitopes (IAd, Rothbard/Taylor pattern),and had 10 B cell epitopes, suggested that the protein can be used as a vaccine candidate. ORF124 gene hasbeen expressed in Escherichia coli under pET32-a(+)vector.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2012-06-03</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/force-download</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/7850</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.7850</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 17, No 1 (2012)</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/7850/6091</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2015 Indonesian Journal of Biotechnology</dc:rights>
</oai_dc:dc>