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Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia

Indonesian Journal of Biotechnology · Vol. 22 No. 1 (2017) · pp. 49–54

DOI
10.22146/ijbiotech.26401
ISSN
2089-2241, 0853-8654
Published
2017-06-01
Publisher
Universitas Gadjah Mada
Language
eng
License
creativecommons.org/licenses/by-sa/4.0

Abstract

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

Keywords

Collaboration network of the authors

40 authors · 101 co-authorship links · showing the 40 most connected

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  • Universitas Trisakti
  • IPB University
  • Universitas Gadjah Mada
  • Affiliation unknown
  • Different organisations
  • Same organisation
  • Affiliation unknown
  • Thicker line = more joint articles · bigger circle = more articles
Show as table
Co-authorOrganisationJoint articles
Ardaning NurilianiAffiliation unknown4
Woro Anindito Sri TunjungAffiliation unknown4
M. MurwantokoAffiliation unknown3
LANGKAH SEMBIRINGAffiliation unknown3
Didik IndradewaAffiliation unknown3
Firman AlamsyahAffiliation unknown3
Alfi Rizqi AmaliaAffiliation unknown3
Pudji AstutiUniversitas Trisakti2
Charlie Ester de FretesAffiliation unknown2
Rifqi Zahroh JanatunaimAffiliation unknown2
Radhiyah Mardhiyah HamidAffiliation unknown2
Tri Rini NuringtyasAffiliation unknown2
Donny WidiantoAffiliation unknown2
Alfino SebastianAffiliation unknown2
Hanum Mukti RahayuAffiliation unknown2
Wahyu Aristyaning PutriAffiliation unknown2
Anis Uswatun KhasanahAffiliation unknown2
Yuni SineAffiliation unknown2
Riris Istighfari JenieAffiliation unknown2
Taryono TaryonoIPB University2
Ratna Dewi EskundariAffiliation unknown2
Masashi KawaichiAffiliation unknown2
Endang SemiartiAffiliation unknown2
Khamidun KhamidunAffiliation unknown1
Rita MalizaAffiliation unknown1
Putra SantosoAffiliation unknown1
Bramadi AryaAffiliation unknown1
Alimuddin TofrizalAffiliation unknown1
Suwarno HadisusantoUniversitas Gadjah Mada1
HaryantiAffiliation unknown1
Sari Budi Moria SembiringAffiliation unknown1
Jhon Harianto HutapeaAffiliation unknown1
I Nyoman Adiasmara GiriAffiliation unknown1
Ketut MahardikaAffiliation unknown1
Bima MahendraAffiliation unknown1

Affiliations come from OpenAlex; journal OAI-PMH metadata carries none. Authors without an OpenAlex ID are matched by name, which can merge or split people.

Metadata source: OAI-PMH (metadata jurnal) https://jurnal.ugm.ac.id/ijbiotech/oai, first harvested 8 Nov 2026, last seen 8 Nov 2026.
Original metadata (oai_dc) 
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	<dc:title xml:lang="en-US">Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia</dc:title>
	<dc:creator>Apridamayanti, Pratiwi</dc:creator>
	<dc:creator>Pratiwi, Rarastoeti</dc:creator>
	<dc:creator>Purwestri, Yekti Asih</dc:creator>
	<dc:creator>Sri Tunjung, Woro Anindito</dc:creator>
	<dc:creator>Rumiyati, Rumiyati</dc:creator>
	<dc:subject xml:lang="en-US">nthocyanin; antioxidant; black rice bran; nutrient contents</dc:subject>
	<dc:description xml:lang="en-US">The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.</dc:description>
	<dc:publisher xml:lang="en-US">Universitas Gadjah Mada</dc:publisher>
	<dc:contributor xml:lang="en-US"></dc:contributor>
	<dc:date>2017-06-01</dc:date>
	<dc:type>info:eu-repo/semantics/article</dc:type>
	<dc:type>info:eu-repo/semantics/publishedVersion</dc:type>
	<dc:type xml:lang="en-US">Original Research Articles</dc:type>
	<dc:format>application/pdf</dc:format>
	<dc:identifier>https://journal.ugm.ac.id/ijbiotech/article/view/26401</dc:identifier>
	<dc:identifier>10.22146/ijbiotech.26401</dc:identifier>
	<dc:source xml:lang="en-US">Indonesian Journal of Biotechnology; Vol 22, No 1 (2017); 49-54</dc:source>
	<dc:source>2089-2241</dc:source>
	<dc:source>0853-8654</dc:source>
	<dc:language>eng</dc:language>
	<dc:relation>https://journal.ugm.ac.id/ijbiotech/article/view/26401/19527</dc:relation>
	<dc:rights xml:lang="en-US">Copyright (c) 2017 The Author(s)</dc:rights>
	<dc:rights xml:lang="en-US">http://creativecommons.org/licenses/by-sa/4.0</dc:rights>
</oai_dc:dc>